scholarly journals Detection and Identification of Plant Growth Promoting Bacteria from Sorghum (Sorghum bicolor L. Moench) Rhizosphere Soil in Northern Ethiopia

2021 ◽  
Vol 18 (3) ◽  
pp. 497-515
Author(s):  
Birhanu Babiye ◽  
Beira H. Meressa ◽  
Taye Tessema
Keyword(s):  
Plant Growth ◽  
Sorghum Bicolor ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Production Test ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Sorghum Production ◽  
Sorghum Bicolor L

Plant growth promoting rhizobacteria are the bacteria which subsist inside and outside of the plant tissue and promote plant growth through direct or indirect mechanisms. To increase sorghum production and productivity we utilize herbicides and chemical fertilizers to overcome sorghum production constraints, but those chemicals have negative side effects. The current study was conducted with the objective of isolation of PGPR from sorghum rhizosphere and screening for primary growth related trait, evaluation of potential PGPR at greenhouse for sorghum growth performance and identify through biochemical characterization. So that, in this study a total of 117 plant growth promoting rhizobacteria were isolated from the rhizosphere of 12 sorghum (Sorghum bicolor L. Moench) genotype by cultivating using 3 collected soil samples from the northern part of Ethiopia (Amhara and Tigray regional states) in greenhouse. Isolated bacteria were screened for primary growth promoting traits such as phosphate solubilization test, IAA production test at different concentration of L-tryptophan and ammonia production test. From the isolated bacteria 28% solubilized Phosphorous, 78% produced IAA at different concentration of tryptophan. The greatest IAA production was scored at 100 mg/L of tryptophan and the lowest production of IAA was scored at 150 mg/L of tryptophan, 69% of isolated bacteria produced ammonia. Hence, 15% of isolated bacteria fulfilled the above primary screening test and used for further greenhouse evaluation. Accordingly, eighteen bacteria were tested for greenhouse experiment using completely randomized design and all 18 isolates were significantly increased all the agronomic parameter as compared to the control such as plant shoot height, plant shoot fresh and dry weight, root length, root fresh and dry weight at p < 0.01 and P ≤ 0.001. Two isolates G6E29 and G4E19 had significantly increased all the parameter but two isolates (G12E19 and G3E40) were statistically non-significant for root fresh weight compared to the control. These 18 potential isolates were characterized morphologically and biochemically. Eight isolates were grouped at Pseudomonas genera. Six isolates were grouped at Azotobacter and the rest four isolates were grouped at Bacillus genera. Thus, the use of plant growth promoting rhizosphere bacteria could be useful to improve sorghum production and productivity. However, further molecular identification and evaluation of the isolates exhibiting multiple plant growths promoting traits on plant-microbe interaction for economic crop of Ethiopia is needed to uncover their efficacy as effective plant growth promoting rhizosphere bacteria.

scholarly journals Perlakuan Rizobakteri Pemacu Pertumbuhan Tanaman (RPPT) dengan Beberapa Tingkat Kerapatan Inokulum Rizobakteri Terhadap Viabilitas dan Vigor Benih Cabai Merah Kadaluarsa (Capsicum annuum L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 229-238
Author(s):  
Dayang Rahmanita Simanjuntak ◽  
Halimursyadah Halimursyadah ◽  
Syamsuddin Syamsuddin
Keyword(s):  
Plant Growth ◽  
Capsicum Annuum ◽  
Seed Treatment ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Seed Vigor ◽  
Inoculum Density ◽  
Plant Growth Promoting ◽  
Actinobacillus Suis ◽  
Growth Promoting

Abstrak. Biological seed treatment merupakan salah satu perlakuan benih menggunakan mikroorganisme seperti rizobakteri pemacu pertumbuhan tanaman (RPPT). Penelitian ini bertujuan untuk mengetahui jenis rizobakteri dan kerapatan inokulum yang dapat meningkatkan viabilitas dan vigor benih cabai kadaluarsa. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) pola faktorial dengan 2 faktor dan 3 ulangan. Faktor pertama adalah jenis rizobakteri (R) terdiri atas lima taraf yaitu R1: Necercia sp; R2:Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. Faktor kedua adalah kerapatan inokulum rizobakteri terdiri dari tiga taraf yaitu K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. Hasil penelitian ini menunjukkan bahwa perlakuan benih menggunakan rizobakteri jenis Necercia sp dengan kerapatan inokulum 108 cfu/ml nyata meningkatkan vigor benih pada tolok ukur  indeks vigor yaitu 40% dan Pseudomonas capacia dengan kerapatan inokulum 109 cfu/ml juga merupakan kombinasi perlakuan terbaik dalam meningkatkan berat kering kecambah normal yaitu 69,33 mg.Treatment Of Plant Growth Promoting Rhizobacteria (PGPR)With Multiple Levels of Rhizobacteria Inoculum Density On Viability and Vigor Of Expired Red Chilli Seeds (Capsicum annuum L.Abstract. Biological seed treatment is one of the seed treatment using microorganisms such as plant growth-promoting rhizobacteria (PGPR). This study aims to determine the type of rhizobacteria and inoculum density that can increase the viability and vigor of expired chili seeds. This research uses Completely Randomized Design (CRD) factorial pattern with 2 factors and 3 replications. The first factor is the type of rhizobacteria (R) consists of five levels, namely R1: Necercia sp; R2: Bacillus polymixa; R3: Actinobacillus suis; R4: Azotobacter sp; R5: Pseudomonas capacia. The second factor is the density of rhizobacteria inoculum consisting of three levels namely K1: 107 cfu/ml; K2: 108 cfu/ml; K3: 109 cfu/ml. The results of this study showed that the seed treatment using the Necercia sp-type rizobacteria with 108 cfu/ml inoculum density significantly increased the seed vigor on the vigor index benchmark of 40% and Pseudomonas capacia with 109cfu/ml inoculum density was also the best treatment combination in increasing dry weight normal sprout is 69,33 mg. 

Field evaluation of asparagus crowns and germinating seeds inoculated with plant growth-promoting rhizobacteria

2009 ◽  
Vol 89 (6) ◽  
pp. 1133-1138
Author(s):  
S M Liddycoat ◽  
D J Wolyn
Keyword(s):  
Water Stress ◽  
Plant Growth ◽  
Field Evaluation ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Asparagus Officinalis ◽  
Shoot Height ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Germinating Seeds

Plant growth-promoting rhizobacteria (PGPR) have the ability to enhance growth and establishment by reducing stress ethylene, or providing exogenous indol-3-acetic acid (IAA), nitrogen, iron, or phosphorus. In previous greenhouse studies, PGPR inoculation of asparagus seeds or seedlings enhanced growth under optimal and water stress conditions. Experiments were conducted in this study to determine if PGPR could provide benefits to transplanted crowns and germinating seeds in the field. Plant growth-promoting rhizobacteria did not positively affect shoot height, count, or diameter of inoculated crowns, or percent germination and seedling dry weight of inoculated seeds. The limitations of using PGPR in the field and potential for future success are discussed.Key words: Asparagus officinalis, Pseudomonas , water stress, drought, irrigation

scholarly journals Effect of plant growth promoting rhizobacteria (PGPR) and IBA treatments on rooting in cuttings of apple (Malus × domestica Borkh.) clonal rootstock Merton 793

2017 ◽  
Vol 9 (2) ◽  
pp. 1135-1138
Author(s):  
Pramod Verma ◽  
P. S. Chauhan ◽  
J. S. Chandel
Keyword(s):  
Plant Growth ◽  
Bacillus Licheniformis ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Bacillus Sp ◽  
Main Shoot ◽  
Primary Roots ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Different Strains

The preliminary studies on the effect of different strains of plant growth promoting rhizobacteria (PGPR) alone and in combination with IBA at 1000 ppm on rooting in cuttings of apple clonal rootstock Merton 793 were carried out during 2012-13. The PGPR strains (RG (1)3 – Bacillus sp.), B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. alone failed to induce rooting response in cuttings of apple clonal rootstock Merton 793. The results revealed that IBA 2500 ppm recorded the maximum rooting (65 %), number of primary roots (5.00), length (28.43 cm) and diameter (3.25 mm) of primary roots, fresh (3.67 g) and dry weight (2.59 g) of roots, length of main shoot (134.14cm), diameter of main shoot (8.18 mm), fresh (30.40 g) and dry weight (22.60 g) of shoots in cuttings of Merton 793. However, the PGPR strains RG (1)3 – Bacillus sp., B6 – Bacillus licheniformis and R3 (3) – Sirretia sp. in combination with IBA 1000 ppm showed improvement in rooting of cuttings to the extent of 10, 15 and 5 per cent rooting, respectively and growth of the rooted plants. IBA at 2500 ppm resulted better rooting and growth of rooted plants. Hence, this treatment is suggested for commercial propagation of apple clonal rootstock Merton 793 through cuttings.

scholarly journals Uji Penggunaan PGPR (Plant Growth-Promoting Rhizobacteria) terhadap Pertumbuhan Vegetatif Tanaman Cabai Merah (capsicum Annuum L.)

Jurnal MIPA ◽  
2019 ◽  
Vol 8 (3) ◽  
pp. 150
Author(s):  
Lidyanti Ollo ◽  
Parluhutan Siahaan ◽  
Beivy Kolondam
Keyword(s):  
Plant Growth ◽  
Plant Height ◽  
Capsicum Annuum ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Root Volume ◽  
Plant Growth Promoting ◽  
Wet Weight ◽  
Number Of Leaves ◽  
Growth Promoting

Penelitian ini bertujuan yang menguji Perbandingan pertumbuhan vegetatif tanaman cabai merah (capsicum Annuum L.) akibat Pemberian Komposisi PGPR (Plant Growth-Promoting Rhizobacteria) yang perbeda. Pertumbuhan tanaman diukur berdasarkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman. Hasil penelitian menujukkan bahwa PGPR dapat meningkatkan tinggi tanaman, jumlah daun, volume akar, berat basah dan berat kering tanaman cabe.Tinggi tanaman terbaik setelah diperlakuan selama 28 hari adalah dengan pemberian PGPR disertai dengan pemberian pupuk kandang dengan tinggi (24.44 cm), yang diikuti oleh pemberian PGPR + kompos dengan tinggi (22.04 cm), dan pemberian PGPR + NPK dengan tnggi tanaman 19.68 cm . Volume akar akan lebih tinggi bila diberikan PGPR yang ditambah dengan pupuk kandang , atau kompos maupun NPK, tetapi tdak berbeda hasilnya bila hanya diberikan PGPR saja. Jumlah daun dapat ditingkatkan bila diberikan PGPR yang ditambah dengan pupuk kandang, atau kompos maupun NPK. Pemberian PGPR + NPK tidak memberikan jumlah daun yang berbeda dengan pemberian PGPR saja.Berat basah akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basah.Berat kering akan meningkat bila diberikan PGPR saja maupun ditambah dengan pemberian pupuk kandang, atau kompos maupun NPK. PGPR ditambah dengan pupuk kandang maupun kompos memberikan dampak yang sama terhadap berat basahThis study aimed to examine the comparison of vegetative growth of red chili (capsicum Annuum L.) due to the administration of different PGPR (Plant Growth-Promoting Rhizobacteria) Composition. Plant growth was measured based on plant height, number of leaves, root volume, wet weight and dry weight of the plant. The results showed that PGPR can increase plant height, number of leaves, root volume, wet weight and dry weight of chilli plants. The best plant height after being treated for 28 days is by giving PGPR accompanied by administering manure with height (24.44 cm), followed by by giving PGPR + compost with a height (22.04 cm), and giving PGPR + NPK with a plant height of 19.68 cm. Root volume will be higher if given PGPR added with manure, or compost or NPK, but the results are not different if only given PGPR. The number of leaves can be increased if given PGPR added with manure, or compost or NPK. Provision of PGPR + NPK does not provide a number of leaves that are different from the administration of PGPR alone. Wet weight increased when given PGPR alone or added with manure, or compost or NPK. PGPR added with manure or compost gives the same effect on wet weight. Dry weight will increase if given PGPR alone or added with manure, or compost or NPK. PGPR coupled with manure or compost has the same effect on wet weight

A gnotobiotic bioassay for studying interactions between potatoes and plant growth-promoting rhizobacteria

1997 ◽  
Vol 43 (9) ◽  
pp. 801-808 ◽  
Author(s):  
Kenneth L. Conn ◽  
George Lazarovits ◽  
Jerzy Nowak
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Potato Cultivar ◽  
Fold Increase ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Single Node ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Potato Plantlets

A gnotobiotic bioassay, using potato plantlets derived from single-node explants and grown in test tubes containing potato nodal cutting medium (PNCM), was found to be highly useful for investigations of direct growth promotion by a nonfluorescent Pseudomonas sp. strain PsJN. Strain PsJN was used to optimize and evaluate this bioassay for purposes of screening other rhizosphere bacteria and identification of Tn5 mutants of strain PsJN deficient in growth-promoting properties. The selection of potato cultivar used in this bioassay was critical, as growth promotion of potatoes by strain PsJN was cultivar specific. Inoculated plantlets of cultivars Norchip, Kennebec, Shepody, and Chaleur showed, in root dry weight, a five- to eight-fold increase, two- to three-fold increase, no response, and a decrease of 50%, respectively. Haulm dry weight followed similar trends but was not as consistent an indicator of growth promotion. Bioassay results were not altered to any extent by minor changes in PNCM composition or by slight changes in temperature and light conditions. A rapid method for preparation of bacterial suspensions and inoculation of explants was developed. Inoculation of three explants taken from 6-week-old stock plantlets of cv. Kennebec for each Tn5 transconjugate of strain PsJN (total of 1500 transconjugates) enabled the elimination of 93% of those isolates that retained growth-promoting activity. The remaining 7% of isolates were retested and seven were confirmed to have lost growth-promoting ability. Bacteria from different genera were also screened with this bioassay. None of these bacteria increased the growth of potato plantlets, but several inhibited root and haulm growth.Key words: plant growth-promoting rhizobacteria, gnotobiotic, tissue culture, nonfluorescent pseudomonad, bacterium, potato.

scholarly journals Alleviation of Salt Stress on Wheat (Triticum aestivum L.) by Plant Growth Promoting Bacteria strains Bacillus halotolerans MSR-H4 and Lelliottia amnigena MSR-M49

2020 ◽  
pp. 44-58
Author(s):  
Ibrahim El-Akhdar ◽  
Tamer Elsakhawy ◽  
Hanaa A. Abo-Koura
Keyword(s):  
Salt Stress ◽  
Plant Growth ◽  
Crop Production ◽  
Halophilic Bacteria ◽  
Wheat Plant ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Plant Growth Promoting Bacteria ◽  
Plant Growth Promoting ◽  
Growth Promoting

The plant growth-promoting rhizobacteria (PGPR) application could reduce the use of synthetic fertilizers and increase the sustainability of crop production. Halophilic bacteria that have PGPR characteristics can be used in different environmental stresses. Two different strains isolated, purified, characterized as a PGPRs and phylogenetic identification using 16sRNA which was revealed to be closest matched at 99% with Bacillus halotolerans and Lelliottia amnigena. The isolates possessed plant growth promoting properties as exopolysaccharides (EPS) and indole acetic acid (IAA) production, Bacillus halotolerans had the ability to fix elemental nitrogen and the two strains have the ability to P-solubilization. Furthermore, the strains were evaluated in alleviation of different levels of salt stress on wheat plant at two experiments (Pots and a Field). Strains under study conditions significantly increased the plant height, straw dry weight (DW g plant-1), spike number, 1000 grain DW recorded 31.550 g with Lelliottia amnigena MSR-M49 compared to un-inoculated and other strain in field,  grain yield recorded 2.77 (ton fed-1) with Lelliottia amnigena  as well as N% and protein content in grains recorded 1.213% and 6.916 respectively with  inoculation with Lelliottia amnigena,  also, spikes length, inoculated wheat show reduction in both proline accumulation in shoots and roots especially with Lelliottia amnigena recorded 2.79 (mg g-1DW), inoculation significantly increased K+ in root-shoot, K+/Na+ in root-shoot and reduced Na+ in root-shoot compared with control. This confirmed that this consortium could provide growers with a sustainable approach to reduce salt effect on wheat production.

scholarly journals Extraction of Indole-3-acetic Acid from Plant Growth Promoting Rhizobacteria of Bamboo Rhizosphere and Its Effect on Biosynthesis of Chlorophyll in Bamboo Seedlings

2020 ◽  
Author(s):  
Janardan Lamichhane ◽  
Bishnu Maya K.C. ◽  
Dhurva Prasad Gauchan ◽  
Sanjay Nath Khanal ◽  
Sharmila Chimouriya
Keyword(s):  
Acetic Acid ◽  
Plant Growth ◽  
Biochemical Analysis ◽  
Chlorophyll Biosynthesis ◽  
Plant Growth Promoting Rhizobacteria ◽  
Agitation Rate ◽  
Iaa Production ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Indole 3 Acetic Acid

Background: Indole-3-acetic acid (IAA), a principal phytohormone, controls several crucial physiological processes of plants. It ameliorates plant growth by stimulating cell elongation, root initiation, seed germination and seedling growth. Alteration of IAA level by plant growth promoting rhizobacteria leads to varied impacts on plant growth and development. Methods: Soil samples were collected from bamboo (Bambusa tulda, B. nutans subsp. cupulata, B. balcooa and Dendrocalamus strictus) rhizosphere. Altogether five bacterial isolates were screened by serial dilution method and subjected to biochemical analysis. The isolate BUX1 with high IAA production capacity was optimized for IAA production. IAA was partially purified and quantified from the bacterial extract by thin layer chromatography (TLC). The influence of extracted bacterial IAA on chlorophyll biosynthesis in bamboo seedlings of B. tulda was compared with uninoculated control plants. Results: Biochemical analysis revealed that all the isolates belonged to genus Bacillus which were found capable of producing IAA. During optimization, BUX1 isolate produced 99.13 µg ml-1 of IAA at 37°C, pH 7, 3 mg l-1 concentration of L-tryptophan and 150 rpm agitation rate after 192 hour of incubation. The Rf value of the bacterial IAA during TLC was identical to that of standard IAA (0.425) indicating that IAA was present in crude extract of Bacillus (BUX1). The influence of bacterial IAA on chlorophyll biosynthesis in bamboo seedlings was significant in comparison to uninoculated plants. Therefore, this isolate could be a prospective candidate to be employed as biofertilizer.

scholarly journals Identification of Plant Growth Promoting Rhizobacteria That Improve the Performance of Greenhouse-Grown Petunias under Low Fertility Conditions

Plants ◽  
2021 ◽  
Vol 10 (7) ◽  
pp. 1410
Author(s):  
Kaylee A. South ◽  
Nathan P. Nordstedt ◽  
Michelle L. Jones
Keyword(s):  
Plant Growth ◽  
Growth Promotion ◽  
Genomic Analysis ◽  
Dry Weight ◽  
Plant Growth Promoting Rhizobacteria ◽  
Low Fertility ◽  
Plant Performance ◽  
Flower Bud ◽  
Plant Growth Promoting ◽  
Growth Promoting

The production of greenhouse ornamentals relies on high fertilizer inputs to meet scheduling deadlines and quality standards, but overfertilization has negative environmental impacts. The goals of this study were to identify plant-growth-promoting rhizobacteria (PGPR) that can improve greenhouse ornamental crop performance with reduced fertilizer inputs, and to identify the best measurements of plant performance for assessing the beneficial impact of PGPR on ornamentals. A high-throughput greenhouse trial was used to identify 14 PGPR isolates that improved the flower/bud number and shoot dry weight of Petunia × hybrida ‘Picobella Blue’ grown under low fertility conditions in peat-based media. These 14 PGPR were then applied to petunias grown under low fertility conditions (25 mg L−1 N). PGPR-treated plants were compared to negative (untreated at 25 mg L−1 N) and positive (untreated at 50, 75, 100, and 150 mg L−1 N) controls. Multiple parameters were measured in the categories of flowering, vegetative growth, and vegetative quality to determine the best measurements to assess improvements in ornamental plant performance. Caballeronia zhejiangensis C7B12-treated plants performed better in almost all parameters and were comparable to untreated plants fertilized with 50 mg L−1 N. Genomic analysis identified genes that were potentially involved in plant growth promotion. Our study identified potential PGPR that can be used as biostimulants to produce high-quality greenhouse ornamentals with lower fertilizer inputs.

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