Protective Effect of Chickpea Protein Hydrolysates on Colon Carcinogenesis Associated With a Hypercaloric Diet

The relationship between hydrophobicity and the protective effect of whey protein hydrolysates (WPHs) against oxidative stress was studied. Whey protein was first hydrolysed by pepsin and trypsin to obtain WPHs. After absorbed by macroporous adsorption resin DA201-C, three fractions named as M20, M40, and M60 were eluted by various concentrations of ethanol. The hydrophobicity showed a trend of increase from M20 to M60. Antioxidant ability test in vitro indicated that all the three components of WPHs displayed reasonably good antioxidant ability. Moreover, with the increase of hydrophobicity, antioxidant ability of WPHs improved significantly. Then rat pheochromocytoma line 12 (PC12) cells oxidative model was built to evaluate the suppression of oxidative stress of three components on PC12 cells induced by H2O2. Morphological alterations, cell viability, apoptosis rate, and intracellular antioxidase system tests all indicated that WPHs exert significant protection on PC cells against H2O2-induced damage. Among them, M60 had the highest protective effect by increasing 19·3% cell survival and reducing 28·6% cell apoptosis. These results suggested hydrophobicity of WPHs was contributing to the antioxidant ability and the protective effect against oxidative damage.

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Protective effect of porcine plasma protein hydrolysates on the gelation of porcine myofibrillar protein exposed to a hydroxyl radical-generating system

International Journal of Biological Macromolecules ◽

10.1016/j.ijbiomac.2017.09.036 ◽

2018 ◽

Vol 107 ◽

pp. 654-661 ◽

Cited By ~ 4

Author(s):

Haili Niu ◽

Yichun Chen ◽

Huan Zhang ◽

Baohua Kong ◽

Qian Liu

Keyword(s):

Protective Effect ◽

Hydroxyl Radical ◽

Plasma Protein ◽

Myofibrillar Protein ◽

Protein Hydrolysates ◽

Generating System

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Obesity, insulin resistance and diabetes in the sand rat exposed to a hypercaloric diet; possible protective effect for IL1-β

Comptes Rendus Biologies ◽

10.1016/j.crvi.2012.03.003 ◽

2012 ◽

Vol 335 (4) ◽

pp. 271-278 ◽

Cited By ~ 12

Author(s):

Ali Khalkhal ◽

Aomar Haddar ◽

Nesrine Semiane ◽

Aicha Mallek ◽

Abdelouadoud Abdelmalek ◽

...

Keyword(s):

Insulin Resistance ◽

Protective Effect ◽

Sand Rat ◽

Hypercaloric Diet

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Protective effect of whey protein hydrolysates against oxidative stress in d-galactose-induced ageing rats

International Dairy Journal ◽

10.1016/j.idairyj.2013.08.004 ◽

2014 ◽

Vol 34 (1) ◽

pp. 80-85 ◽

Cited By ~ 13

Author(s):

Xinyan Peng ◽

Baohua Kong ◽

Haiyang Yu ◽

Xinping Diao

Keyword(s):

Oxidative Stress ◽

Protective Effect ◽

Whey Protein ◽

Protein Hydrolysates ◽

Whey Protein Hydrolysates

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Study of the antineoplastic action of Tabebuia avellanedae in carcinogenesis induced by azoxymethane in mice

Acta Cirurgica Brasileira ◽

10.1590/s0102-86502011000200009 ◽

2011 ◽

Vol 26 (2) ◽

pp. 125-128 ◽

Cited By ~ 7

Author(s):

Roberta Alves Higa ◽

Ricardo Dutra Aydos ◽

Iandara Schettert Silva ◽

Rondon Tosta Ramalho ◽

Albert Schiaveto de Souza

Keyword(s):

Protective Effect ◽

Colon Carcinogenesis ◽

Group Iv ◽

Aberrant Crypt Foci ◽

Antitumor Action ◽

Group V ◽

Group Iii ◽

Antineoplastic Action ◽

Group I ◽

Group Ii

PURPOSE: To study the antitumor action of Tabebuia avellanedae in experimentally induced colon carcinogenesis by azoxymethane in mice. METHODS: Fifty (n=50) mice were divided into five groups: in group I azoxymethane (AOM) was administered, in Group II - β-lapachone, in group III - vehicle (diluent) and in group IV - vehicle + AOM and finally in group V - β-lapachone + AOM. RESULTS: It was observed the presence of aberrant crypt foci in all animals of groups I and IV, 50% in group II and 90% in group V. CONCLUSION: The β-lapachone extracted from the Tabebuia avellanedae showed no protective effect of lesions induced by azoxymethane in colon of mice.

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