scholarly journals A new monoclonal antibody against rhamnogalacturonan II and its application to immunocytochemical detection of rhamnogalacturonan II in Arabidopsis roots

2018 ◽  
Vol 82 (10) ◽  
pp. 1780-1789 ◽  
Author(s):  
Ye Zhou ◽  
Masaru Kobayashi ◽  
Tatsuya Awano ◽  
Toru Matoh ◽  
Keiji Takabe
Keyword(s):  
Monoclonal Antibody ◽  
Immunocytochemical Detection ◽  
Rhamnogalacturonan Ii

Immunocytochemical detection of bone marrow micrometastases in colorectal carcinoma patients, using a monoclonal antibody to villin

Cytometry ◽  
2001 ◽  
Vol 46 (5) ◽  
pp. 281-289 ◽  
Author(s):  
Roman Rouzier ◽  
Edwige Bourstyn ◽  
Fr�d�ric Grozier ◽  
Anne Berger ◽  
Daniel Louvard ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Colorectal Carcinoma ◽  
Immunocytochemical Detection ◽  
Bone Marrow Micrometastases

scholarly journals Chromogranin in bronchopulmonary neuroendocrine cells. Immunocytochemical detection in human, monkey, and pig respiratory mucosa.

1987 ◽  
Vol 35 (1) ◽  
pp. 113-118 ◽  
Author(s):  
J M Lauweryns ◽  
L van Ranst ◽  
R V Lloyd ◽  
D T O'Connor
Keyword(s):  
Monoclonal Antibody ◽  
Silver Staining ◽  
Chromogranin A ◽  
Neuroendocrine Cells ◽  
Neuroepithelial Bodies ◽  
Apud Cells ◽  
Respiratory Mucosa ◽  
Immunocytochemical Detection

Immunoreactive chromogranin A was demonstrated by immunocytochemistry in the cytoplasm of neuroendocrine cells (NEC) and neuroepithelial bodies (NEB) in human, monkey, and pig respiratory mucosa. Three different antisera (one monoclonal and one polyclonal to human chromogranin A, and one polyclonal to bovine chromogranin A) were applied in this study. Chromogranin immunopositivity varied in extent and intensity according to the antiserum applied and the tissue investigated. The monoclonal antibody revealed the strongest immunoreaction. Good correlation between chromogranin immunoreactivity and Grimelius silver staining was observed by comparing adjacent sections, although more cells seemed to reveal chromogranin immunoreactivity than argyrophylia. Chromogranin appears to be a useful histological marker for APUD cells in the respiratory mucosa of several species.

scholarly journals Immunocytochemical detection of rhamnogalacturonan II on forming cell plates in cultured tobacco cells

2017 ◽  
Vol 81 (5) ◽  
pp. 899-905 ◽  
Author(s):  
Ye Zhou ◽  
Tatsuya Awano ◽  
Masaru Kobayashi ◽  
Toru Matoh ◽  
Keiji Takabe
Keyword(s):  
Tobacco Cells ◽  
Immunocytochemical Detection ◽  
Rhamnogalacturonan Ii

scholarly journals Immunocytochemical detection of serotonin with monoclonal antibodies.

1981 ◽  
Vol 29 (12) ◽  
pp. 1425-1430 ◽  
Author(s):  
A Consolazione ◽  
C Milstein ◽  
B Wright ◽  
A C Cuello
Keyword(s):  
Monoclonal Antibody ◽  
Monoclonal Antibodies ◽  
Brain Tissue ◽  
Heavy Chain ◽  
Light Chains ◽  
Immunocytochemical Detection ◽  
Binding Antibody ◽  
High Concentrations

The development of the monoclonal antibody YC5/45 HLK (YC5/HLK) against a 5HT-bovine seroalbumin immunogen and its application for immunocytochemistry is described. The YC5/HLK antibody is the product of a rat x rat hybrid myeloma, producing a heavy chain and two light chains. In hemagglutination tests, the antibody cross-reacts to entirety with dopamine, serotonin, and tryptamine at high concentrations. The serotonin-albumin conjugate is 20,000 times more effective in displacing the binding antibody, while albumin itself goes unrecognized by the antibody. In fixed preparations of brain tissue, immunofluorescence is observed only in neurons known to contain serotonin, while no reaction is observed in dopamine-rich neurons. All immunofluorescence is extinguished by the use of agents that inhibit the biosynthesis of 5HT, but not of the catecholamines.

A web-like network of type vi collagen in human skin is revealed by immuno-electron microscopy

1988 ◽  
Vol 46 ◽  
pp. 382-383
Author(s):  
Douglas R. Keene ◽  
Robert W. Glanville ◽  
Eva Engvall
Keyword(s):  
Electron Microscopy ◽  
Monoclonal Antibody ◽  
Human Skin ◽  
Basement Membranes ◽  
Primary Antibody ◽  
Fat Cells ◽  
Secondary Antibody ◽  
Type Vi Collagen ◽  
Dermal Matrix ◽  
Immuno Electron Microscopy

A mouse monoclonal antibody (5C6) prepared against human type VI collagen (1) has been used in this study to immunolocalize type VI collagen in human skin. The enbloc method used involves exposing whole tissue pieces to primary antibody and 5 nm gold conjugated secondary antibody before fixation, and has been described in detail elsewhere (2).Biopsies were taken from individuals ranging in age from neonate to 65 years old. By immuno-electron microscopy, type VI collagen is found to be distributed as a fine branching network closely associated with (but not attached to) banded collagen fibrils containing types I and III collagen (Fig. 1). It appears to enwrap fibers, to weave between individual fibrils within a fiber, and to span the distance separating fibers, creating a “web-like network” which entraps fibers within deep papillary and reticular dermal layers (Fig. 2). Relative to that in the dermal matrix, the concentration of type VI collagen is higher around endothelial basement membranes limiting the outer boundaries of nerves, capillaries, and fat cells (Fig. 3).

Immunocytochemical detection of hepatic carbohydrate metabolic enzymes: Improved resolution with polyethylene glycol embedding and visio-bond semithin sections

1992 ◽  
Vol 50 (1) ◽  
pp. 792-793
Author(s):  
Kuixiong Gao ◽  
Randal E. Morris ◽  
Bruce F. Giffin ◽  
Robert R. Cardell
Keyword(s):  
Polyethylene Glycol ◽  
Glycogen Phosphorylase ◽  
Phosphoenolpyruvate Carboxykinase ◽  
Glycogen Synthase ◽  
Metabolic Enzymes ◽  
Silver Stain ◽  
Accurate Localization ◽  
Immunocytochemical Detection ◽  
Semithin Sections ◽  
Parenchymal Cells

Several enzymes are involved in the regulation of anabolic and catabolic pathways of carbohydrate metabolism in liver parenchymal cells. The lobular distribution of glycogen synthase (GS), phosphoenolpyruvate carboxykinase (PEPCK) and glycogen phosphorylase (GP) was studied by immunocytochemistry using cryosections of normal fed and fasted rat liver. Since sections of tissue embedded in polyethylene glycol (PEG) show good morphological preservation and increased detectability for immunocytochemical localization of antigenic sites, and semithin sections of Visio-Bond (VB) embedded tissue provide higher resolution of cellular structure, we applied these techniques and immunogold-silver stain (IGSS) for a more accurate localization of hepatic carbohydrate metabolic enzymes.

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