Evaluation of the compatibility of Isaria fumosorosea (Hypocreales: Cordycipitaceae) IF-1106 with several adjuvants

2021 ◽  
pp. 1-14
Author(s):  
Hongliang Diao ◽  
Jing Tian ◽  
Peixiang Xing ◽  
Conglin Wang ◽  
Zhihui Han ◽  
...  
Keyword(s):  
Isaria Fumosorosea

Effects of wetting on the persistence of quiescent conidia of Isaria fumosorosea

2010 ◽  
Vol 55 (3) ◽  
pp. 203-210 ◽  
Author(s):  
Najah Bouamama ◽  
Claire Vidal ◽  
Jacques Fargues
Keyword(s):  
Isaria Fumosorosea

scholarly journals Pathogenicity of Isaria fumosorosea to European red mite in the laboratory

Biotemas ◽  
2017 ◽  
Vol 30 (1) ◽  
pp. 73
Author(s):  
Cláudia Andréia Gräff ◽  
Liana Johann ◽  
Cláucia Fernanda Volken de Souza ◽  
Noeli Juarez Ferla
Keyword(s):  
Rio Grande ◽  
Panonychus Ulmi ◽  
Rio Grande Do Sul ◽  
Isaria Fumosorosea ◽  
European Red Mite

http://dx.doi.org/10.5007/2175-7925.2017v30n1p73A vitivinicultura no estado do Rio Grande do Sul tem sofrido infestações significativas de ácaros praga, destacando-se recentemente Panonychus ulmi (Koch). Há relatos sobre a capacidade de controle de ácaros fitófagos com Isaria fumosorosea. O objetivo deste trabalho foi avaliar em laboratório a ação patogênica de I. fumosorosea sobre o ácaro P. ulmi. As criações de P. ulmi foram estabelecidas a partir de coleta realizadas em videiras da Serra Gaúcha. Sobre os ovos de P. ulmi, as aplicações de suspensões de esporos foram feitas em diferentes concentrações. As triplicatas das fêmeas de 12 a 15 dias foram tratadas com suspensão a 108 esporos x mL-1. As testemunhas foram tratadas com água destilada. Após sete dias da aplicação, observaram-se 55,6% de ovos não eclodidos tratados com suspensão 106 esporos x mL-1 e com fêmeas tratadas obteve-se uma mortalidade total entre 85-90% e mortalidade confirmada entre 50-55%. A mortalidade máxima dos controles no tratamento dos ovos e das fêmeas foi, em média, de 12,8 e 15,5%, respectivamente. Conclui-se que o isolado I. fumosorosea possui habilidade para infectar ovos e fêmeas adultas de P. ulmi e portanto mostra-se como uma alternativa viável para ser experimentada no campo.

scholarly journals Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

2010 ◽  
Vol 53 (4) ◽  
pp. 753-761 ◽  
Author(s):  
Gabriel Moura Mascarin ◽  
Sérgio Batista Alves ◽  
Rogério Biaggioni Lopes
Keyword(s):  
Mass Production ◽  
Culture Media ◽  
Fungal Species ◽  
Complete Medium ◽  
Sugarcane Molasses ◽  
Liquid Media ◽  
Isaria Fumosorosea ◽  
Isaria Farinosa ◽  
Selection For ◽  
Biphasic Fermentation

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and whole rice and broken rice for I. fumosorosea. Results demonstrated that the two fungal species could be rapidly produced with higher yield of conidia on agro-industrial resources by using biphasic fermentation techniques.

Evaluation of the Entomopathogenic Fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea for Control of Sitophilus oryzae

2014 ◽  
Vol 77 (1) ◽  
pp. 87-93 ◽  
Author(s):  
NICKOLAS G. KAVALLIERATOS ◽  
CHRISTOS G. ATHANASSIOU ◽  
MARIA M. AOUNTALA ◽  
DEMETRIUS C. KONTODIMAS
Keyword(s):  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Metarhizium Anisopliae ◽  
Galleria Mellonella ◽  
Sitophilus Oryzae ◽  
Exposure Period ◽  
Isaria Fumosorosea ◽  
Stored Grain ◽  
Stored Grain Pest ◽  
Petri Dishes

The entomopathogenic fungi Beauveria bassiana, Metarhizium anisopliae, and Isaria fumosorosea were tested against the stored-grain pest Sitophilus oryzae. The fungi were isolated from the soil (from three locations in Attica, Greece: B. bassiana from Tatoion, M. anisopliae from Marathon, and I. fumosorosea from Aghios Stefanos) using larvae of Galleria mellonella as bait. Suspensions of 2.11 × 107 and 2.11 × 108, 1.77 × 107 and 1.77 × 108, and 1.81 × 107 and 1.81 × 108 conidia per ml of B. bassiana, M. anisopliae, and I. fumosorosea, respectively, were applied by three treatments: (i) sprayed on food and set in petri dishes with adults of S. oryzae, (ii) sprayed on adults of S. oryzae and set in petri dishes without food, and (iii) sprayed on adults of S. oryzae and set in petri dishes with food. The observed mortality of S. oryzae adults during the overall exposure period for the lowest, as well as for the highest, concentrations of B. bassiana, M. anisopliae, and I. fumosorosea ranged from 0 to 100%. Concentration was, in most of the cases tested, a critical parameter that determined the “speed of kill” of the exposed insect species for B. bassiana and M. anisopliae. Conversely, concentration was not that critical for I. fumosorosea, and survival was high in some of the combinations tested, even after 14 days of exposure. Both in the highest and the lowest concentrations of fungi, the mortality of S. oryzae adults was higher when the fungi were applied on adults than when they were applied on food. Higher mortality was observed when food was absent than when food was present, in most of the cases tested. The high efficacy levels recorded in the current study indicate that the tested fungi could be effective biocontrol agents against S. oryzae.

scholarly journals Dissemination of Isaria fumosorosea Spores by Steinernema feltiae and Heterorhabditis bacteriophora

2020 ◽  
Vol 6 (4) ◽  
pp. 359
Author(s):  
Jiří Nermuť ◽  
Jana Konopická ◽  
Rostislav Zemek ◽  
Michal Kopačka ◽  
Andrea Bohatá ◽  
...  
Keyword(s):  
Entomopathogenic Nematodes ◽  
Heterorhabditis Bacteriophora ◽  
Potato Dextrose Agar ◽  
Steinernema Feltiae ◽  
Soil Organisms ◽  
Isaria Fumosorosea ◽  
Second Stage ◽  
Glass Tubes ◽  
First Time ◽  
Globally Distributed

Entomopathogenic nematodes and fungi are globally distributed soil organisms that are frequently used as bioagents in biological control and integrated pest management. Many studies have demonstrated that the combination of biocontrol agents can increase their efficacy against target hosts. In our study, we focused on another potential benefit of the synergy of two species of nematodes, Steinernema feltiae and Heterorhabditis bacteriophora, and the fungus Isaria fumosorosea. According to our hypothesis, these nematodes may be able to disseminate this fungus into the environment. To test this hypothesis, we studied fungal dispersal by the nematodes in different arenas, including potato dextrose agar (PDA) plates, sand heaps, sand barriers, and glass tubes filled with soil. The results of our study showed, for the first time, that the spreading of both conidia and blastospores of I. fumosorosea is significantly enhanced by the presence of entomopathogenic nematodes, but the efficacy of dissemination is negatively influenced by the heterogeneity of the testing arena. We also found that H. bacteriophora spread fungi more effectively than S. feltiae. This phenomenon could be explained by the differences in the presence and persistence of second-stage cuticles or by different foraging behavior. Finally, we observed that blastospores are disseminated more effectively than conidia, which might be due to the different adherence of these spores (conidia are hydrophobic, while blastospores are hydrophilic). The obtained results showed that entomopathogenic nematodes (EPNs) can enhance the efficiency of fungal dispersal.

scholarly journals Low Efficacy of Isaria fumosorosea against Box Tree Moth Cydalima perspectalis: Are Host Plant Phytochemicals Involved in Herbivore Defence against Fungal Pathogens?

2020 ◽  
Vol 6 (4) ◽  
pp. 342
Author(s):  
Rostislav Zemek ◽  
Jana Konopická ◽  
Zain Ul Abdin
Keyword(s):  
Host Plant ◽  
Fungal Pathogens ◽  
Invasive Pest ◽  
Isaria Fumosorosea ◽  
Fungus Infection ◽  
Hydroalcoholic Extract ◽  
Ornamental Tree ◽  
Temperature Scanning ◽  
Fungus Growth ◽  
Herbivore Defence

Buxus sp. is an important native and ornamental tree in Europe threatened by a serious invasive pest Cydalima perspectalis. The larvae of this moth are able to defoliate box trees and cause their death. The development of novel biopesticides targeting this pest might help protect Buxus trees grown wildly or in city parks. Laboratory experiments were conducted to assess the efficacy of entomopathogenic fungus Isaria fumosorosea strain CCM 8367 against C. perspectalis. The last-instar larvae of the box tree moth were treated by the suspension of fungus conidia at concentrations ranging from 1 × 104 to 1 × 108 spores per 1 mL. Fungus infection was observed mostly in pupae, but the maximum mortality did not exceed 60%, indicating a very low susceptibility of C. perspectalis to I. fumosorosea. Furthermore, a number of ungerminated fungal conidia were found on larval cuticles using a low-temperature scanning electron microscopy. Our data also reveal that the hydroalcoholic extract from B. sempervirens leaves significantly inhibits both the germination of I. fumosorosea conidia and fungus growth. It can be speculated that the strain CCM 8367 of I. fumosorosea is not a potent biocontrol agent against C. perspectalis and low virulence of the fungus might be due to the accumulation of host plant phytochemicals having antimicrobial activity in larval cuticle of the pest.

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