scholarly journals Culture media selection for mass production of Isaria fumosorosea and Isaria farinosa

2010 ◽  
Vol 53 (4) ◽  
pp. 753-761 ◽  
Author(s):  
Gabriel Moura Mascarin ◽  
Sérgio Batista Alves ◽  
Rogério Biaggioni Lopes
Keyword(s):  
Mass Production ◽  
Culture Media ◽  
Fungal Species ◽  
Complete Medium ◽  
Sugarcane Molasses ◽  
Liquid Media ◽  
Isaria Fumosorosea ◽  
Isaria Farinosa ◽  
Selection For ◽  
Biphasic Fermentation

This work investigated the production of the fungi Isaria fumosorosea and Isaria farinosa in biphasic fermentation using agro-industrial products and residues. Combinations of natural liquid substrates, alternative to the complete medium and potato dextrose medium, were evaluated. The best liquid media were sugarcane molasses + rice broth, rice broth + yeast and sugarcane molasses + yeast + rice broth, which resulted in the highest viable propagule concentration. The molasses + rice broth medium was selected for the next phase of the study in which the production of both fungal isolates was evaluated in solid grain substrates. In solid-state fermentation, the best conidia production was achieved with the soybean meal and broken corn for I. farinosa, and whole rice and broken rice for I. fumosorosea. Results demonstrated that the two fungal species could be rapidly produced with higher yield of conidia on agro-industrial resources by using biphasic fermentation techniques.

Optimal mass production technology for sporulation of Verticillium lecanii and Trichoderma harzianum

2015 ◽  
Vol 4 (1) ◽  
pp. 296-302
Author(s):  
Saba Hasan
Keyword(s):  
Production Technology ◽  
Trichoderma Harzianum ◽  
Mass Production ◽  
Fungal Species ◽  
Biocontrol Agents ◽  
Verticillium Lecanii ◽  
Complete Medium ◽  
Liquid Media ◽  
Solid Substrates ◽  
Viability Percentage

The present study aims at optimization of a suitable mass production technology for growth and sporulation of the biocontrol agents Verticillium lecanii and Trichoderma harzianum. Combinations of various natural solid substrates (Rice, Maize, Arhar, Defatted Soybean, Gram) and laboratory media (SMYA, Complete media, SDA, Czapek’s Dox media ) were evaluated for growth and sporulation of V. lecanii and T. harzianum. Data on CFU, concentration of propagules, dry mycelial weight, conidia production and conidia yield were analyzed. It was observed that among the substrate types tested, the fungal strains grew better and produced high quantity of spores in Rice as growth medium after 21 days of incubation followed by Maize and Arhar. The best liquid media which supported maximum conidial production was SMYA and Complete medium. Also, rate of germination of spores as well as their viability at different aeration rates was tested. It was observed that maximum length of germ tube in minimum duration was observed in Rice as substrate. The viability percentage of the biocontrol agents produced at various aeration rates and agitation speeds showed that change in aeration and agitation did not significantly affect spore viability. Results demonstrated that the fungal species could be rapidly produced with a high conidial yield on natural solid substrates as compared to liquid media by fermentation technology.

scholarly journals Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia

2021 ◽  
Vol 22 (7) ◽  
Author(s):  
Achmad Arifiyanto ◽  
Endah Setyaningrum ◽  
Nismah Nukmal ◽  
Titik Nur Aeny
Keyword(s):  
Crude Extract ◽  
Short Communication ◽  
Biochemical Characterization ◽  
Culture Media ◽  
Antimalarial Activity ◽  
Mud Volcano ◽  
Broth Culture ◽  
Complete Medium ◽  
Streptomyces Sp

Abstract. Arifiyanto A, Setyaningrum E, Nukmal N, Aeny TN. 2021. Short Communication: In vitro antimicrobial and antimalarial screening of a crude extract of Streptomyces sp. AB8 isolated from Lapindo Mud Volcano Area, Sidoarjo, Indonesia. Biodiversitas 22: 2817-2823. Streptomyces is a potential bacterial genus that has been investigated extensively as a source of natural microbial compounds. Its potential metabolites have been widely developed for pharmaceutical, pathogen control, and other applications in agriculture. This study aimed to determine the ability of the Streptomyces sp AB8 crude extract in inhibiting Plasmodium and pathogenic microbes.  Streptomyces was cultured on Gause synthetic media for 10 days. The fermented broth culture media has dissolved in a 1:1 mixture of ethyl acetate and methanol. Biochemical characterization of this isolate has carried out using the standard methods. In-vitro antimalarial activity assay was performed using a chloroquine-sensitive Plasmodium falciparum strain 3D7. Fresh type O-positive human erythrocytes were suspended at 4 percent hematocrit in a complete medium to maintain culture. The inhibitory concentration (IC50) was determined using probit analysis. The results showed the extract of Streptomyces sp. AB8 contains phenolic and alkaloids. Streptomyces sp. AB8 extract can inhibit Dickeya zeae N-Unila 5, Dickeya zeae N-Unila 10, Aspergillus sp IK3, and Escherichia coli growth. The results also showed that the ICs0 value of extract against P. falciparum 3D7 was 17.56 ug/mL. Further research was needed to determine the types of purified bioactive compounds and their bioactivity.

Culture media for mass production of microalgae

2019 ◽  
pp. 33-50 ◽  
Author(s):  
Júlio Cesar de Carvalho ◽  
Eduardo Bittencourt Sydney ◽  
Lorenzo Ferrari Assú Tessari ◽  
Carlos Ricardo Soccol
Keyword(s):  
Mass Production ◽  
Culture Media

scholarly journals Evaluation of spore inoculum and confirmation of pathway genetic blueprint of T13αH and DBAT from a Taxol-producing endophytic fungus

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Balabhadrapatruni V. S. K. Chakravarthi ◽  
Satpal Singh ◽  
Subban Kamalraj ◽  
Vijai Kumar Gupta ◽  
Chelliah Jayabaskaran
Keyword(s):  
Fusarium Solani ◽  
Endophytic Fungus ◽  
Competitive Inhibition ◽  
Liquid Media ◽  
Alternative Source ◽  
Natural Origin ◽  
Baccatin Iii ◽  
Enhanced Production ◽  
Significant Research ◽  
Selection For

AbstractTaxol (paclitaxel), a plant-derived anticancer drug, has been among the most successful anticancer drugs of natural origin. Endophytic fungi have been proposed as a prominent alternative source for Taxol and its intermediate Baccatin III, however the very low yields remain a hinderance to their commercial utilization. Significant research efforts towards this end are underway globally. Here, we report the results on our earlier reported Taxol-producing endophytic fungus, Fusarium solani from the standpoint of spores as seed inoculum and media selection for enhanced Taxol and baccatin III yields. Spores produced on M1D medium with 94.76% viability were used for further media optimization for Taxol and Baccatin III production in five different liquid media under static and shaker condition at different cultivation days. Taxol and Baccatin III when quantified through competitive inhibition enzyme immunoassay (CIEIA), showed maximum production at 136.3 µg L−1 and 128.3 µg L−1, respectively in the modified flask basal broth (MFBB) under shaking condition. Further, two important genes of this pathway, namely taxane 13α-hydroxylase (T13αH) and 10-deacetylbaccatin III-10-β-O-acetyltransferase (DBAT) have been identified in this fungus. These findings are hoped to assist in further manipulation and metabolic engineering of the parent F. solani strain towards the enhanced production of Taxol and baccatin III.

scholarly journals Culture Medium, Light Regime and Temperature Affect the Development of Sirosporium diffusum

2018 ◽  
Vol 10 (6) ◽  
pp. 310
Author(s):  
Tales Poletto ◽  
Marlove F. B. Muniz ◽  
Vinícius S. Fantinel ◽  
Renata F. Favaretto ◽  
Igor Poletto ◽  
...  
Keyword(s):  
Culture Medium ◽  
Culture Media ◽  
Fungal Species ◽  
Light Regime ◽  
Leaf Spot Disease ◽  
Light Regimes ◽  
Physical Conditions ◽  
Randomized Design ◽  
Brown Leaf Spot ◽  
Brown Leaf

Sirosporium diffusum is the causal agent of the brown leaf spot disease on pecan trees that seriously damages the foliage of adult plants and seedlings. This fungal species is difficult to grow satisfactorily in a culture medium. Therefore, the aim of this study was to evaluate the effects of different physical conditions on the development of S. diffusum. In the first assay, eight culture media and five light regimes were combined, while in the second, the three treatments that promoted highest sporulation were combined with three temperatures. The trials were conducted in a two-factorial arrangement in a fully randomized design with six replicates. V8, V8CaCO3, and CA media under a 24-h photoperiod produced the highest respective sporulations: 29 × 104, 35 × 104, and 41 × 104 conidia ml-1. The best temperature for sporulation was 20±1 °C for all culture media, especially V8CaCO3 and CA. The best artificial conditions for obtaining good mycelial growth and sporulation consisted of a photoperiod of 24 h, temperature of 20±1 °C and V8CaCO3 or CA culture medium.

Culture of Helicobacter pylori from domestic water samples – the impact of strain variation on growth on solid and in liquid media

2006 ◽  
Vol 54 (3) ◽  
pp. 147-152 ◽  
Author(s):  
R.J. Owen ◽  
S.A. Chisholm ◽  
G. Brick ◽  
J.V. Lee ◽  
S. Surman-Lee ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Culture Media ◽  
Tap Water ◽  
Clinical Isolates ◽  
Strain Variation ◽  
Liquid Media ◽  
Domestic Water ◽  
Source Of Infection ◽  
Solid Media ◽  
The Impact

Helicobacter pylori is an important global human pathogen and there is growing evidence from PCR assays that contaminated drinking water might be a possible source of infection in some circumstances. There are no validated protocols for direct isolation but various culture media have been developed for possible environmental sampling. Our aim here was to investigate how inter-strain variation might affect the interpretation of results with such media. Two laboratory adapted reference strains and four recent clinical isolates were tested on four solid media and in ten liquid media. Considerable variation was found between strains in their ability to recover on the different media after stress exposure (suspension in sterile tap water). Generally, clinical isolates were less robust than the laboratory-adapted strains and, overall, the former required longer recovery times. Our findings highlighted the importance of using a range of isolates for evaluations, as examination of laboratory-adapted strains alone did not provide an accurate representation of the utility of media that may be used to recover H. pylori from water.

Mass multiplication of Metarhizium anisopliae on different media

2020 ◽  
Vol 5 (01) ◽  
pp. 138-144
Author(s):  
Ankush Taliyan ◽  
Ajay Kumar ◽  
Rajendra Singh ◽  
Rohit Rana ◽  
Shekhar Rana
Keyword(s):  
Pest Management ◽  
Entomopathogenic Fungi ◽  
Mass Production ◽  
Metarhizium Anisopliae ◽  
Fungal Species ◽  
Mass Multiplication

Entomopathogenic fungi (EPF) Metarhizium anisopliae are fungal species that is the frequently occurring and destructive pest management to the pathogenic soil and insects. The effect of different substrates for the mass production of Metarhizium anisopliae spore/ml was significantly higher recorded The results revealed that all the treatments were significantly producing spore per ml and thus increasing the yield significantly as compared to other substrates. The results revealed that all the treatments were significantly higher effective in producing spore/ml as compared to other substrates overall finding showed that substrate tested, for Metarhizium anisopliae spore/ml production was significantly higher recorded 240.53 and 195.26 spore/ml were recorded on substrate irrespective of the temperature.

scholarly journals Mutualism breakdown in breadfruit domestication

2011 ◽  
Vol 279 (1731) ◽  
pp. 1122-1130 ◽  
Author(s):  
Xiaoke Xing ◽  
Alexander M. Koch ◽  
A. Maxwell P. Jones ◽  
Diane Ragone ◽  
Susan Murch ◽  
...  
Keyword(s):  
Arbuscular Mycorrhizas ◽  
Fungal Species ◽  
Plant Domestication ◽  
Unintended Effects ◽  
Below Ground ◽  
Selection For ◽  
Root Symbionts ◽  
Mycorrhizal Response ◽  
Mutualism Breakdown

During the process of plant domestication, below-ground communities are rarely considered. Some studies have attempted to understand the changes in root symbionts owing to domestication, but little is known about how it influences mycorrhizal response in domesticated crops. We hypothesized that selection for above-ground traits may also result in decreased mycorrhizal abundance in roots. Breadfruit ( Artocarpus sp.) has a long domestication history, with a strong geographical movement of cultivars from west to east across the Melanesian and Polynesian islands. Our results clearly show a decrease in arbuscular mycorrhizas (AMs) along a domestication gradient from wild to recently derived cultivars. We showed that the vesicular and arbuscular colonization rate decreased significantly in more recently derived breadfruit cultivars. In addition, molecular analyses of breadfruit roots indicated that AM fungal species richness also responded along the domestication gradient. These results suggest that human-driven selection for plant cultivars can have unintended effects on below-ground mutualists, with potential impacts on the stress tolerance of crops and long-term food security.

Incidence and stability of infection by double-stranded RNA genetic elements inAspergillussectionflaviand effects on aflatoxigenicity

1996 ◽  
Vol 74 (5) ◽  
pp. 716-725 ◽  
Author(s):  
Karol S. Elias ◽  
Peter J. Cotty
Keyword(s):  
Nitrogen Metabolism ◽  
Culture Media ◽  
Aflatoxin Production ◽  
Double Stranded Rna ◽  
Agarose Gels ◽  
Genetic Elements ◽  
Electrophoretic Migration ◽  
Fungal Isolates ◽  
Nit Mutants ◽  
Selection For

Ninety-two isolates belonging to Aspergillus sect, flavi were analyzed for double-stranded (ds) RNA via standard cellulose chromatography. Double-stranded RNA infection was detected in fungal isolates that had been in culture for long periods (5 of 26 were infected) and in those recently isolated (5 of 66 were infected). The number of dsRNA genetic elements differed among infected isolates and no two isolates contained identical dsRNAs on the basis of electrophoretic migration in agarose gels. Addition of micronutrients to culture media affected both the amount of dsRNA produced and the number of dsRNA genetic elements detected. Attempts to cure six fungal isolates of dsRNA by serial single conidial transfer, chlorate selection for nitrogen-metabolism mutants, and cycloheximide treatment, met with variable results. The frequency at which serial single conidial transfer and nitrogen-metabolism mutant (nit) selection successfully cured six Aspergillus sect, flavi isolates varied from 11 to 100% and 0 to 100%, respectively. The cycloheximide treatment was effective at curing 40% of the dsRNA-infected isolates. Comparison of aflatoxin production prior to and after dsRNA curing indicated that infection by dsRNA did not influence aflatoxin production. However, aflatoxin production by two isolates (91-031B and 91-184G) was reduced by both single conidial transfer and induction of nit mutants. Keywords: aflatoxin, cycloheximide, mycovirus, nitrogen-metabolism mutant.

scholarly journals Sorption of Zinc by exopolysaccharides produced by liquid media of phytopatogenic fungi

2021 ◽  
Vol 26 (1) ◽  
pp. 2312-2317
Author(s):  
JUAN DIEGO VALENZUELA-COBOS ◽  
ANA GRIJALVA-ENDARA
Keyword(s):  
Yeast Extract ◽  
Liquid Culture ◽  
Culture Medium ◽  
Colletotrichum Gloeosporioides ◽  
Culture Media ◽  
Phytopathogenic Fungi ◽  
Mycelial Biomass ◽  
Rhizopus Stolonifer ◽  
Liquid Media ◽  
Liquid Culture Medium

Phytopathogenic fungi such as: Colletotrichum gloeosporioides and Rhizopus stolonifer were cultivated in three different liquid culture media: LCC (glucose 40 g L-1 , yeast extract 3 g L-1 ), LC2 (glucose 40 g L-1 , yeast extract 3 g L-1 and tryptone peptone 2 g L-1 ) and LC3 (glucose 40 g L-1 , yeast extract 3 g L-1 and tryptone peptone 10 g L-1 ) under pH of 5.5 for the production of mycelial biomass and exopolysaccharides (EPS). The liquid culture medium (LC3) used in cultivation of Colletotrichum gloeosporioides showed the highest production of biomass (15.40 g L-1 ) and exopolysaccharides (3.40 g L-1 ). Exopolysaccharides (EPS) obtained from the liquid culture medium (LC3) of Colletotrichum gloeosporioides presented the highest absorption content of Zinc (56 mg g-1 ). The results presented that the exopolysaccharides (EPS) produced by Colletotrichum gloeosporioides showed the greatest biosorbent capacity of Zinc (Zn) using the culture medium with the highest amount of tryptone peptone.

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