Soil washing of total petroleum and polycyclic aromatic hydrocarbons from crude oil contaminated ultisol using aqueous extracts of waterleaf

Background: Stenotrophomonas are ubiquitous gram-negative bacteria which survive in a wide range of environments. They can use many substances for their growth and are known to be intrinsically resistant to many antimicrobial agents. They have been tested for biotechnological applications, bioremediation and antimicrobial agents because of their recalcitrant nature to many toxic compounds. Method. Stenotrophomonas sp. Pemsol was isolated from a crude oil contaminated soil. The capability of this isolate to tolerate and degrade polycyclic aromatic hydrocarbons (PAHs) (anthracene, anthraquinone, biphenyl, naphthalene, phenanthrene, phenanthridine and xylene) was evaluated on Bush Nell Hass medium containing PAHs as the unique carbon sources. The metabolites formed after 30-day degradation of naphthalene by Pemsol were analyzed using Fourier Transform Infra-red Spectroscopic (FTIR), Ultra-Performance Liquid Chromatography-Mass Spectrometry (UPLC-MS) and Gas Chromatography-Mass Spectrometry (GC-MS). Results. Complete degradation of naphthalene at a concentration of 1 mg/mL was obtained and a newly formed catechol peak obtained from the UPLC-MS and GC-MS confirmed the degradation. The strain Pemsol lacked the ability to produce biosurfactant so that it cannot bio-emulsify PAHs. The whole genome analysis of Stenotrophomonas sp. Pemsol revealed a wealth of genes for hydrocarbon utilization and interaction with the environment and the presence of 147 genes associated with the degradation of PAHs, some of which are strain-specific on the genomic islands. Few genes are associated with bio-emulsification indicated that Pemsol without biosurfactant production has a genetic basis. This is the first report of the complete genome analysis sequence of a PAH-degrading Stenotrophomonas. Stenotrophomonas sp. Pemsol possesses features that makes it a good bacterium for genetic engineering and will therefore be a good tool for the remediation of crude oil or PAH-contaminated soil.

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Assessment of the biodegradation capacity of Azolla on polycyclic aromatic hydrocarbons in crude oil

Global NEST Journal ◽

10.30955/gnj.002544 ◽

2018 ◽

Vol 20 (3) ◽

pp. 465-470

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Crude Oil ◽

Aromatic Hydrocarbons ◽

Oil Spills ◽

Dependent Manner ◽

Measured Intensity ◽

Azolla Filiculoides ◽

Concentration Dependent Manner ◽

Plant Samples ◽

Polycyclic Aromatic

<p>In this study, the potential use of Azolla filiculoides Lam. for the bioremediative solution to polycyclic aromatic hydrocarbon (PAH) pollution due to crude oil spills in freshwater was investigated. The plants were grown in nitrogen-free Hoagland nutrient solution media containing 0.05%, 0.1%, 0.2%, 0.3%, 0.4% and 0.5% crude oil by volume for 15 days under greenhouse conditions. Relative growth rates of A. filiculoides decreased in the presence of crude oil in a concentration-dependent manner. The probable influence of A. filiculoides on the biodegradation of polycyclic aromatic hydrocarbons was measured by using synchronous UV fluorescence spectroscopy. GC-MS analysis were also carried out to elucidate the behavior of the oil in experimental and control samples. Although 1-2 rings PAHs have not been encountered in control or plant samples, the measured intensity for 3-4 ring PAHs in plant samples was remarkably lower in comparison to the control. Furthermore, these results demonstrated that the predominant efficacy of the A. filiculoides was for 3-4 ring PAHs at the range 0.05 to 0.2% crude oil concentrations. It could be concluded that the bioremediative potential of A. filiculoides for the removal of polycyclic aromatic hydrocarbons strongly depends on the amount of oil in the contaminated water resource. In other words, A. filiculoides could be used more effectively after the removal of excess crude oil in the spilled freshwater areas.</p>

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Application of Soil Washing Treatment Method for the Remediation of Petroleum Polluted Soil

Handbook of Research on Resource Management for Pollution and Waste Treatment - Advances in Environmental Engineering and Green Technologies ◽

10.4018/978-1-7998-0369-0.ch020 ◽

2020 ◽

pp. 476-501

Author(s):

Abdullahi Evuti Mohammed ◽

Kamoru Adio Salam ◽

Silas Shamaye Samuel

Keyword(s):

Polycyclic Aromatic Hydrocarbons ◽

Aromatic Hydrocarbons ◽

Contaminated Soils ◽

Soil Washing ◽

Treatment Method ◽

Petroleum Products ◽

Gas Chromatography Mass Spectrometry ◽

Detergent Concentration ◽

Polycyclic Aromatic ◽

Percentage Removal

The increasing contamination of soil by petroleum products has been a great source of concern to our society because of its negative consequences on the environment. Thus, several remediation technologies and trials have been propounded for a crude oil-polluted environment. This chapter reviews the dynamics of pollutants in the soil and the various treatment technologies for petroleum-polluted soils viz physico-chemical, thermal, and biological treatment methods. Authors experimented on soil washing using detergent for the remediation of petroleum contaminated soils considering different concentrations. The percentage removal of aliphatic and Polycyclic Aromatic Hydrocarbons (PAHs) was determined using Gas Chromatography Mass Spectrometry (GC-MS). The highest percentage removal efficiencies of 97.55% and 61.41% for aliphatic and Polycyclic Aromatic Hydrocarbons were obtained at detergent concentration of 20w/v% respectively.

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