Isolation and Sequencing of Glycosyltransferase Gene and UDP-glucose Dehydrogenase Gene that are Located on a Gene Cluster Involved in a New Exopolysaccharide Biosynthesis in Streptomyces

DNA Sequence ◽  
2003 ◽  
Vol 14 (2) ◽  
pp. 141-145 ◽  
Author(s):  
Lingyan Wang ◽  
Shitao Li ◽  
Yuan Li
Keyword(s):  
Gene Cluster ◽  
Glucose Dehydrogenase ◽  
Glycosyltransferase Gene ◽  
Dehydrogenase Gene ◽  
Exopolysaccharide Biosynthesis

scholarly journals Identification and Molecular Characterization of the Chromosomal Exopolysaccharide Biosynthesis Gene Cluster from Lactococcus lactis subsp. cremoris SMQ-461

2005 ◽  
Vol 71 (11) ◽  
pp. 7414-7425 ◽  
Author(s):  
N. Dabour ◽  
G. LaPointe
Keyword(s):  
Gene Cluster ◽  
Lactococcus Lactis ◽  
Repeat Unit ◽  
Raw Milk ◽  
Open Reading Frames ◽  
Pcr Analysis ◽  
Biosynthesis Gene Cluster ◽  
Glycosyltransferase Gene ◽  
Reverse Transcription Pcr ◽  
Exopolysaccharide Biosynthesis

ABSTRACT The exopolysaccharide (EPS) capsule-forming strain SMQ-461 of Lactococcus lactis subsp. cremoris, isolated from raw milk, produces EPS with an apparent molecular mass of >1.6 × 106 Da. The EPS biosynthetic genes are located on the chromosome in a 13.2-kb region consisting of 15 open reading frames. This region is flanked by three IS1077-related tnp genes (L. lactis) at the 5′ end and orfY, along with an IS981-related tnp gene, at the 3′ end. The eps genes are organized in specific regions involved in regulation, chain length determination, biosynthesis of the repeat unit, polymerization, and export. Three (epsGIK) of the six predicted glycosyltransferase gene products showed low amino acid similarity with known glycosyltransferases. The structure of the repeat unit could thus be different from those known to date for Lactococcus. Reverse transcription-PCR analysis revealed that the eps locus is transcribed as a single mRNA. The function of the eps gene cluster was confirmed by disrupting the priming glycosyltransferase gene (epsD) in Lactococcus cremoris SMQ-461, generating non-EPS-producing reversible mutants. This is the first report of a chromosomal location for EPS genetic elements in Lactococcus cremoris, with novel glycosyltransferases not encountered before in lactic acid bacteria.

scholarly journals Characterization of the developmentally regulated Bacillus subtilis glucose dehydrogenase gene.

1986 ◽  
Vol 166 (1) ◽  
pp. 238-243 ◽  
Author(s):  
K A Lampel ◽  
B Uratani ◽  
G R Chaudhry ◽  
R F Ramaley ◽  
S Rudikoff
Keyword(s):  
Bacillus Subtilis ◽  
Glucose Dehydrogenase ◽  
Developmentally Regulated ◽  
Dehydrogenase Gene

scholarly journals Function and Regulation of the Formate Dehydrogenase Genes of the Methanogenic Archaeon Methanococcus maripaludis

2003 ◽  
Vol 185 (8) ◽  
pp. 2548-2554 ◽  
Author(s):  
Gwendolyn E. Wood ◽  
Andrew K. Haydock ◽  
John A. Leigh
Keyword(s):  
Gene Expression ◽  
Gene Cluster ◽  
Double Mutant ◽  
Formate Dehydrogenase ◽  
Gene Clusters ◽  
Growth Defect ◽  
Methanococcus Maripaludis ◽  
Gene Sets ◽  
Dehydrogenase Gene ◽  
Duplication Events

ABSTRACT Methanococcus maripaludis is a mesophilic species of Archaea capable of producing methane from two substrates: hydrogen plus carbon dioxide and formate. To study the latter, we identified the formate dehydrogenase genes of M. maripaludis and found that the genome contains two gene clusters important for formate utilization. Phylogenetic analysis suggested that the two formate dehydrogenase gene sets arose from duplication events within the methanococcal lineage. The first gene cluster encodes homologs of formate dehydrogenase α (FdhA) and β (FdhB) subunits and a putative formate transporter (FdhC) as well as a carbonic anhydrase analog. The second gene cluster encodes only FdhA and FdhB homologs. Mutants lacking either fdhA gene exhibited a partial growth defect on formate, whereas a double mutant was completely unable to grow on formate as a sole methanogenic substrate. Investigation of fdh gene expression revealed that transcription of both gene clusters is controlled by the presence of H2 and not by the presence of formate.

scholarly journals A plant binary vector with an antisense soybean UDP-glucose dehydrogenase gene

2003 ◽  
Vol 3 (1) ◽  
pp. 49-52
Author(s):  
A. Borém ◽  
P.M. Olhoft ◽  
L.A. Litterer ◽  
D.W. Plank ◽  
D.A. Somers
Keyword(s):  
Binary Vector ◽  
Glucose Dehydrogenase ◽  
Dehydrogenase Gene

scholarly journals Cloning and characterization of a UDP-glucose dehydrogenase gene from mulberry Broussonetia kazinoki * Broussonetia papyifera

2020 ◽  
Vol 64 ◽  
pp. 667-678
Author(s):  
R.H. JI ◽  
Z. ZHANG ◽  
X. GUO ◽  
Y.L. BAO ◽  
W.B. ZHANG ◽  
...  
Keyword(s):  
Glucose Dehydrogenase ◽  
Dehydrogenase Gene

scholarly journals Cloning and Nucleotide Sequencing of the Membrane-Bound l-Sorbosone Dehydrogenase Gene of Acetobacter liquefaciens IFO 12258 and Its Expression in Gluconobacter oxydans

1995 ◽  
Vol 61 (5) ◽  
pp. 2069-2069
Author(s):  
M Shinjoh ◽  
N Tomiyama ◽  
A Asakura ◽  
T Hoshino
Keyword(s):  
Alcohol Dehydrogenase ◽  
Glucose Dehydrogenase ◽  
Gluconobacter Oxydans ◽  
Pyrroloquinoline Quinone ◽  
Nucleotide Sequencing ◽  
Common Region ◽  
Quinone Binding ◽  
Dehydrogenase Gene ◽  
Membrane Bound

Volume 61, no. 2, p. 419, column 1, lines 15-19: this sentence should read as follows. "The alcohol dehydrogenase and glucose dehydrogenase have a common region reported to be related to pyrroloquinoline quinone binding (2, 10), but SNDH does not contain such a region, indicating that SNDH is not a quinoprotein." Page 419, column 2, line 12: "(Table 4)" should read "(Table 3)." [This corrects the article on p. 413 in vol. 61.].

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