Development and application of an optimized drop-slide technique for metaphase chromosome spreads in maize

2019 ◽  
Vol 95 (4) ◽  
pp. 276-284
Author(s):  
Qilun Yao ◽  
Jian Gao ◽  
Fabo Chen ◽  
Wenbo Li
Keyword(s):  
Metaphase Chromosome ◽  
Slide Technique

Fibrinolysis in Decidual Spiral Arteries in Late Pregnancy

1978 ◽  
Vol 39 (03) ◽  
pp. 751-758 ◽  
Author(s):  
B L Sheppard ◽  
J Bonnar
Keyword(s):  
Electron Microscopy ◽  
Endothelial Cells ◽  
Fibrinolytic Activity ◽  
Physiological Mechanism ◽  
Late Pregnancy ◽  
Full Term ◽  
Fibrin Deposition ◽  
Placental Villi ◽  
The Media ◽  
Slide Technique

SummaryThe fibrinolytic activity of the intimal cells of decidual spiral arteries and the syncytium of placental villi was studied by electron microscopy in ten normal full-term human pregnancies using a modification of the fibrin slide technique. Endothelial cells lining the intima of the decidual spiral arteries showed a considerably greater fibrinolytic activity than intimal cytotrophoblast and the syncytiotrophoblast showed no activity.The replacement of endothelial cells by an intimal lining of cytotrophoblast, and the presence of cytotrophoblast in the media, appears to play an important role in the reduction of the fibrinolytic activity of the vessel. This inhibition of fibrinolytic activity in the utero-placental arteries may be the physiological mechanism which controls fibrin deposition in these vessels and on the placental villi.

Persistence of Fibrinolytic Activity in Fragments of Human Veins Cultured in Vitro

1970 ◽  
Vol 24 (01/02) ◽  
pp. 043-047 ◽  
Author(s):  
M Pandolfi
Keyword(s):  
Blood Vessels ◽  
Plasminogen Activator ◽  
Fibrinolytic Activity ◽  
Vasa Vasorum ◽  
Adult Human ◽  
Slide Technique

SummaryExplants from 5 adult human veins were cultured in a fibrinolytically inactive medium for 3 weeks and assayed for the presence of plasminogen activator by the fibrin slide technique. The explants from 3 veins showed fibrinolytic activity confined to their vasa vasorum for the whole duration of the culture; no decrease of activity was seen. The finding suggests that small blood vessels are able to synthesize plasminogen activator.

scholarly journals GAMMA RAY - INDUCED MITOTIC ABNORMALITIES IN Helianthus annuus L. VARIETY EKIZ 1 / LOS RAYOS DE GAMMA CAUSA ANORMALIDADES MITOTICAS EN Helianthus annuus L. VARIEDAD DE EKIZ 1 / LES ANORMALITÉS MITOTIQUES CHARGÉÉS DES RAYONS GAMMA

Helia ◽  
2001 ◽  
Vol 24 (35) ◽  
pp. 39-46
Author(s):  
Orhan Arslan ◽  
Şenol Bal ◽  
Nilgün Venice ◽  
Semra Mirici
Keyword(s):  
Helianthus Annuus ◽  
Mitotic Index ◽  
Gamma Rays ◽  
Metaphase Chromosome ◽  
Gamma Ray ◽  
Dose Increase ◽  
Helianthus Annuus L ◽  
Mitotic Abnormalities ◽  
Chromosome Stickiness

SUMMARYIn this study, mitotic effects of gamma rays on Ekiz 1 variety belonging to Helianthus annuus L. (2n= 34) in the M0 (first irradiated seeds), M1 and M2 generations have been investigated. Seeds (M0) were irradiated with gamma rays at 10, 20, 30, 40 and 50 kR doses. Percentage of total abnormalities in the M0, M1 and M2 generations increased parallel to the increasing dose of radiation. These abnormalites have been observed as C-metaphase, chromosome stickiness, laggards and bridges with or without fragment. Mitotic index (M.I.) in the M0, M1 and M2 generations has decreased parallel to the dose increase. When the generations are compared, both the amounts of decrease in mitotic index and in the percentage of mitotic abnormalities were mostly observed in M0.

Complementarity of interphase and metaphase chromosome analysis in human renal tumors

1993 ◽  
Vol 6 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Sandra R. Wolman ◽  
Frederic M. Waldman ◽  
Margit Balazs
Keyword(s):  
Metaphase Chromosome ◽  
Chromosome Analysis ◽  
Renal Tumors

Shaping the metaphase chromosome: coordination of cohesion and condensation

BioEssays ◽  
2001 ◽  
Vol 23 (10) ◽  
pp. 924-935 ◽  
Author(s):  
Ana Losada ◽  
Tatsuya Hirano
Keyword(s):  
Metaphase Chromosome

A Novel Technique for Observing the Internal Ultrastructure of Human Chromosomes with Known Karyotype

2008 ◽  
Vol 14 (4) ◽  
pp. 357-361 ◽  
Author(s):  
Mohammad Ghazizadeh ◽  
Yoshihiro Sasaki ◽  
Tatsuo Oguro ◽  
Shigeru Sato ◽  
Seiko Egawa ◽  
...  
Keyword(s):  
Metaphase Chromosome ◽  
Osmium Tetroxide ◽  
Human Lymphocytes ◽  
Fragile Sites ◽  
Human Chromosomes ◽  
Chromosome Research ◽  
Novel Technique ◽  
Transmission Electron ◽  
Comprehensive Information ◽  
Normal Human

Observation of the internal ultrastructure of human chromosomes by transmission electron microscopy (TEM) has frequently been attempted in spite of the difficulties in detaching metaphase chromosome spreads from the glass slide for further processing. In this study we have used a method in which metaphase chromosome spreads were prepared on a flexible thermoplastic membrane (ACLAR) film. To assess chromosome identity, a diamidino-phenylindole staining and karyotying was first done using a conventional cytogenetic system. The chromosome spreads were then fixed with 1% osmium tetroxide, stained with freshly prepared 2% tannic acid, dehydrated, and flat-embedded in epoxy resin. The resin sheet was easily detachable and carried whole chromosome spreads. By this method, TEM observation of chromosomes from normal human lymphocytes allowed a thorough examination of the ultrastructure of centromeres, telomeres, fragile sites, and other chromosomal regions. Various ultrastructural patterns including thick electron dense boundaries, less dense internal regions, and extended chromatin loops at the periphery of the chromosomes were discernible. Application of the present method to chromosome research is expected to provide comprehensive information on the internal ultrastructure of different chromosomal regions in relation to function.

Screening of Ten Plant Species for Metaphase Chromosome Preparation in Adult Mosquitoes (Diptera: Culicidae) Using an Inoculation Technique

1999 ◽  
Vol 36 (6) ◽  
pp. 892-895 ◽  
Author(s):  
A. Jitpakdi ◽  
W. Choochote ◽  
D. Insun ◽  
P. Tippawangkosol ◽  
P. Keha ◽  
...  
Keyword(s):  
Plant Species ◽  
Metaphase Chromosome ◽  
Chromosome Preparation ◽  
Inoculation Technique

C-Microtubules in Isolated Mitotic Spindles

1971 ◽  
Vol 9 (3) ◽  
pp. 603-619
Author(s):  
W. D. COHEN ◽  
T. GOTTLIEB
Keyword(s):  
Cross Section ◽  
Sea Urchin ◽  
Cross Sections ◽  
Metaphase Chromosome ◽  
Inverse Relationship ◽  
Mitotic Spindles ◽  
Cylindrical Structure ◽  
Arbacia Punctulata ◽  
Alternate Possibility

Microtubules with incomplete cylindrical structure are present in isolated mitotic spindles of the sea urchin, Arbacia punctulata. In cross-section they appear C-shaped, and are thus similar to the ‘C-microtubules’ or ‘C-filaments’ observed previously in other systems. The C-microtubules are not uniformly distributed within isolated spindles, but are typically numerous in the interzonal region of anaphase spindles and in the metaphase chromosome ‘plate’. In chromosome-to-pole regions they are seen much less frequently, and microtubules with the usual O-configuration predominate. Counts of C- and O-microtubules in anaphase spindle cross-sections of known location show an inverse relationship between the number of C-microtubules present and the total number of microtubules present. The observations suggest that the C-microtubules are not simple artifacts of fixation or isolation, but rather may represent a stage of microtubule disassembly which occurs in the interzone during isolation or during anaphase in vivo. The alternate possibility of assembly is not excluded, however. The significance of C-microtubules is further discussed with respect to their occurrence in other systems, and to potential differences between mitotic microtubules.

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