Qualitative Estimation of Seed of Butea monosperma Lam. by using Chromatography Technique

The objective of present studies deals with the Qualitative estimation of seed of Butea monosperma Lam. By using Chromatography Technique. The phytochemical study of different extract of seed of Butea monosperma Lam. Were observed various active chemical constituent like phytosterol, flavonoid, saponin and sterol etc. Qualitative estimation of Gallic acid, Rutin and Quarcetin was carried out by HPTLC and HPLC system.

Phytochemical Screening of Bombaxceiba Flowers

Abstract- Traditional knowledge of medicinal plants is showing important and significant values to society. Bombaxceiba is an important medicinal plant of tropical and subtropical India. Its medicinal usage has been reported in the traditional systems of medicine such as Ayurveda, Siddha and Unani. The present study includes the detailed exploration of phytochemical properties of various extracts of Bombaxceiba in an attempt to provide a direction for further research. Flowers of Bombaxceiba were air-dried and its coarsely powdered samples were subjected to Soxhlet extraction using diverse solvents (petroleum ether, chloroform , ethanol, ethyl acetate and distilled water)). Freshly prepared extracts were exposed to standard phytochemical analysis for qualitative estimation of phytoconsituents. Phytochemical analysis r e v e a l e d t h e p r e s e n c e o f s e v e r a l phytochemicals viz., alkaloids, flavonoids, steroids, phenol, tannins, steroid, terpenoids and glycocides. The methanolic extract displ ayed the pr e s enc e of highe st phytochemical compounds. It may be due to the higher solubility of active components in this solvent as compared to other solvents. The studies justify the use of Bombaxceiba in traditional medicines. The investigation further propose that the metabolites present in leaf tissue of Bombaxceiba can be potential source of novel natural antibacterial and antioxidant agents which may be of prospective application in food industry as an antioxidant. Keywords: Phytochemical, Bombaxceiba, Antioxidant

Isolation of Aerobic Bacteria Flora in the Gills and Gastrointestinal Tract of Culturable Freshwater Fish from Ogbia Bayelsa State

Fish is in high demand as food, food additives, and supplements as they are a rich source of carbon, proteins, vitamins, and minerals. Fish has been established to possess bacterial populations on or in their skin, gills, digestive tract, etc. with their microbial diversity often reflecting the bacterial populations of the surrounding water which are either allochthonous or allochthonous. This study isolated and enumerated aerobic bacteria flora in the gastrointestinal tract and gills of four culturable freshwater fish (Silver catfish, Tilapia, Clarias, and Heterobranchus). These species of cultural freshwater fish were obtained and each adult specie held in a separate glass containing unchlorinated water and transferred to the laboratory. The quantitative and qualitative estimation of the bacteria flora present in the gill and Gastro-Intestinal Tract (GIT) of fish were investigated. The Mean total plate count on Nutrient Agar (NA), Blood Agar (BA), MacConkey Agar (MCA), Cysteine-Lactose-Electrolyte-Deficient Agar, (CLED Agar) and Salmonella – Shigella Agar (SSA) was found to be 60 and 40 CFU, 150 and 80 CFU, 100 and 90 CFU and 80 and 70 CFU respectively. Most of the isolates were of public significance. The results showed that fish contains a large number of microbiotas which may play a role in nutrition and health.

Phytochemical and Antimicrobial Activity Screening of StemExtracts of Tinospora cordifolia

Abstract-Tinosporacordifoliaisapopularmedicinal plant which is used in several traditional medicines to cure various diseases. The common names are Amrita and Guduchi, belonging to the family of Menispermaceae. The aim of the study was to study the stem extract of traditional medicinal plant, T. cordifolia for qualitative estimation of phytoconsituents and subsequently to determine its antibacterialactivityagainsttwotestmicroorganisms Escherichia coliand Pseudomonas aeruginosato authenticate its use in traditional medicines. Stems were air-dried and coarsely powdered samples were subjectedto Soxhlet extractionusingdiversesolvents(Hexane,chloroform, ethyl acetate and methanol and water). Freshly prepared extracts were exposed to standard phytochemical analysis for qualitative estimation of phytoconsituents. The antibacterial activity of the stem extract of T. cordifolia was determined by agar well diffusion method. Phytochemical analysis revealed the presence of several phytochemicals viz., alkaloids, flavonoids, steroids, phenol, tannins, steroid, terpenoids, saponins and sugars. The methanolic extract displayed the presence of highest number of phytochemical compounds. It may be due to the higher solubility of active components in this solvent as compared to other solvents. The results revealed that the methanolic extract exhibit the effective antibacterial activity against the tested bacterial species . The studies justify the use of T. cordifolia in traditional medicines. The investigation further proposed that the metabolites present in leaf tissue of T. cordifolia can be potential source of novel natural antibacterial and antioxidant agents and can be prospective applications in food industry as an antioxidant

Evaluating the safety and toxicity of a modified dextran-based biopolymer as a hemostat

Background: As an effective alternative to conventional suture techniques, topical hemostatic agents are widely used to control bleeding and close wounds in surgeries. Objective: This study was conducted to evaluate the efficacy and safety of a novel hydrogel hemostat that is easy to use in applicator under normal conditions according to ISO 10993. Methods: Safety of DexGel, a natural surgical hemostat containing mainly dextran that is an effective hydrogel in bleeding stop, was evaluated and compared to that of AristaTM. APTT test and cytotoxicity tests (i.e., MTT assay, Crystal violet assay, and qualitative estimation) were carried out for considering safety in skin sensitization in a guinea pig model. Results: This study has shown, DexGel does not cause any noticeable sensitization such as edema and erythema or observable toxicity for skin and does not disturb the coagulation process. In addition, cytotoxicity results approve its safety for cell survival. Conclusion: It can be concluded that the safety and efficacy of DexGel (5 g) are comparable or even better to that of AristaTM, which is commercial and frequently used hemostat and the lack of skin irritation and toxicity for DexGel, alleviates initial safety concerns for products based on these polymers and oligomers.