Impacts of carbon source addition on denitrification and phosphorus uptake in enhanced biological phosphorus removal systems

2013 ◽  
Vol 48 (4) ◽  
pp. 464-473 ◽  
Author(s):  
Shamim A. Begum ◽  
Jacimaria R. Batista
Keyword(s):  
Carbon Source ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

Morphology, In-Situ characterization with rRNA targetted probes and respiratory quinone profiles of enhanced biological phosphorus removal sludge

1998 ◽  
Vol 38 (8-9) ◽  
pp. 69-76 ◽  
Author(s):  
I. M. Sudiana ◽  
T. Mino ◽  
H. Satoh ◽  
T. Matsuo
Keyword(s):  
Carbon Source ◽  
Activated Sludge ◽  
Microbial Communities ◽  
Phosphorus Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
In Situ Characterization ◽  
Respiratory Quinone ◽  
Biological Phosphorus

The microbial communities in activated sludge acclimated with either acetate or glucose as the major carbon source under phosphorus limited or rich conditions were investigated morphologically, phylogenetically and chemotaxonomically. The sludge with a minimized polyphosphate content was dominated by tetrad shaped bacteria, which were suspected to be ‘glycogen accumulating bacteria (GAOs) or G bacteria’ The sludge containing high polyphosphate was dominated by cluster forming coccus bacteria. Quinone analyses suggested that all the sludge tested contained various ubiquinones and menaquinones, of which the ubiquinones Q-8 and Q-10 were dominant. Analyses with rRNA targeted probes showed that beta sub class of Proteobacteria was most predominant in all sludges tested. Morphological, phylogenetic and chemotaxonomic investigation all indicated that both high and low P sludges are microbiologically diverse.

The requirement of metal cations for enhanced biological phosphorus removal by activated sludge

1999 ◽  
Vol 40 (2) ◽  
pp. 159-165 ◽  
Author(s):  
Vikram M. Pattarkine ◽  
Clifford W. Randall
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Metal Cations ◽  
Chemical Precipitation ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Batch Experiments ◽  
Biological Phosphorus ◽  
Potassium Magnesium

The objectives of the study described in this paper were to study the requirements of potassium, magnesium, and calcium for enhanced biological phosphorus removal (EBPR) and to determine whether either potassium or magnesium could support EBPR on its own. Batch experiments indicated that phosphorus uptake by the sludge was affected by the availability of potassium, magnesium, and calcium. Both potassium and magnesium were simultaneously required and neither was adequate by itself for EBPR. Calcium did not appear to be required for EBPR, and did not seem to be involved in biologically mediated chemical precipitation.

Microbial community associated with glucose-induced enhanced biological phosphorus removal

2009 ◽  
Vol 60 (8) ◽  
pp. 2105-2113 ◽  
Author(s):  
Guangxue Wu ◽  
Ketil B Sørensen ◽  
Michael Rodgers ◽  
Xinmin Zhan
Keyword(s):  
Microbial Community ◽  
Carbon Source ◽  
Phosphorus Removal ◽  
Lactic Acid Production ◽  
Carbon Substrate ◽  
Molecular Fingerprinting ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Stage 1 ◽  
Biological Phosphorus

The microbial community associated with enhanced biological phosphorus removal with glucose as the main carbon source at 11°C was investigated using microscopy and molecular fingerprinting techniques. The study lasted 77 days and comprised two stages—Stage 1 when the mixture of glucose, yeast and dried milk was the organic carbon source and Stage 2 when glucose was the single carbon source. Rhodocyclus-related polyphosphate accumulating organisms, α-Proteobacteria and Bacteroidetes constituted 42% in Stage 1 and 45% in Stage 2, 21% in Stage 1 and 16% in Stage 2, and 10% in Stage 1 and 7% in Stage 2 of the total Bacteria, respectively. The Trichococcus genus from the low GC Gram-positive bacteria was possibly responsible for lactic acid production from glucose. The microbial community was gradually changing throughout the experiment and appeared to stabilize towards the end of the experiment. Periods of suboptimal phosphorus removal could have been caused by competition among different microbial communities for carbon substrate.

Glutamate as sole carbon source for enhanced biological phosphorus removal

2019 ◽  
Vol 657 ◽  
pp. 1398-1408 ◽  
Author(s):  
Natalia Rey-Martínez ◽  
Marina Badia-Fabregat ◽  
Albert Guisasola ◽  
Juan Antonio Baeza
Keyword(s):  
Carbon Source ◽  
Phosphorus Removal ◽  
Sole Carbon Source ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Biological Phosphorus
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