Isolation and characterisation of the microspore-related gene BcMF4 in Chinese cabbage-pak-choi and its functional identification in Arabidopsis

2007 ◽  
Vol 82 (1) ◽  
pp. 133-139 ◽  
Author(s):  
Lecheng Liu ◽  
Yongqin Wang ◽  
Tao Zhang ◽  
li Huang ◽  
Xun Xiang ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Related Gene ◽  
Functional Identification ◽  
Pak Choi

Chinese Cabbage-pak-choi Transcriptome Map Construction with cDNAAFLP Techniques

2008 ◽  
Vol 7 (10) ◽  
pp. 1181-1188 ◽  
Author(s):  
Shu-ying FAN ◽  
Jian-gang LE ◽  
Guang-jie CHENG ◽  
Cai-jun WU
Keyword(s):  
Chinese Cabbage ◽  
Pak Choi ◽  
Map Construction

A polygalacturonase inhibitory protein gene (BcMF19) expressed during pollen development in Chinese cabbage-pak-choi

2010 ◽  
Vol 38 (1) ◽  
pp. 545-552 ◽  
Author(s):  
Li Huang ◽  
Ying Liu ◽  
Xiaolin Yu ◽  
Xun Xiang ◽  
Jiashu Cao
Keyword(s):  
Chinese Cabbage ◽  
Pollen Development ◽  
Protein Gene ◽  
Inhibitory Protein ◽  
Pak Choi

Cercospora brassicicola. [Descriptions of Fungi and Bacteria].

1982 ◽  
Author(s):  
P. M. Kirk
Keyword(s):  
Sierra Leone ◽  
Geographical Distribution ◽  
Chinese Cabbage ◽  
Solomon Islands ◽  
Indian Mustard ◽  
Crambe Abyssinica ◽  
Pak Choi ◽  
East Indies ◽  
Leaf Spots ◽  
Black Mustard

Abstract A description is provided for Cercospora brassicicola. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: Brassica alba (white mustard), B. alboglabra, B. campestris (bird rape), B. chinensis (chinese cabbage, pak-choi), B. juncea (indian mustard), B. nigra (black mustard), B. oleracea (broccoli, cabbage, cauliflower), B. oleracea var. rapa (turnip), B. parachinensis (mock pak-choi), B. pekinensis (chinese cabbage, pe-tsai), Crambe abyssinica (syn. B. abyssinica: Abyssinian kale). DISEASE: Found associated with pale brown, often dark bordered leaf spots. GEOGRAPHICAL DISTRIBUTION: Africa (Kenya, Malawi, Mauritius, Nigeria, Sierra Leone, Sudan, Tanzania, Togo, Uganda), Asia (Burma, India, Malaya, Sri Lanka), East Indies (Papua New Guinea, Philippines, Sabah, Sarawak, Solomon Islands), West Indies (Antigua, Trinidad). TRANSMISSION: By air-borne and water-borne conidia and via insects (56, 1791).

Establishment of Agrobacterium-mediated genetic transformation and application of CRISPR/Cas9 gene-editing system to Chinese cabbage (Brassica rapa L. ssp. pekinensis)

2020 ◽  
Author(s):  
Xiaonan Li ◽  
Haiyan Li ◽  
Yuzhu Zhao ◽  
Peixuan Zong ◽  
Zongxiang Zhan ◽  
...  
Keyword(s):  
Genetic Transformation ◽  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Transformation Method ◽  
Sequencing Analysis ◽  
Related Gene ◽  
Transformation System ◽  
Regeneration System ◽  
Regeneration Rate ◽  
Functional Studies

Abstract Background: Chinese cabbage, belonging to Brassica rapa species, is an important vegetable in Eastern Asia. It is well known that Chinese cabbage is quite recalcitrant to genetic transformation and the transgenic frequency is generally low. The lack of an efficient and stable genetic transformation system for Chinese cabbage has largely limited related gene functional studies.Results: In this study, we firstly developed a regeneration system for Chinese cabbage by optimizing numerous factors, with 93.50% regeneration rate. Based on this, a simple and efficient Agrobacterium-mediated genetic transformation method was established, without a pre-culture procedure and concentration adjustment of hormone and AgNO3 in co-cultivation and selection media. Using this system, transformants could be obtained within 3.5 to 4.0 months. Average transformation frequency is up to 10.83%. Furthermore, using this transformation system, the CRISPR/Cas9 technology was successfully applied in Chinese cabbage by knocking out a self-incompatibility-related gene SRK. Gene sequencing analysis in the positive transgenic lines revealed various mutations, including deletions, insertions, and substitutions. Conclusion: A simple, stable and efficient genetic transformation method was established for Chinese cabbage and successfully applied to the CRISPR/Cas9 system. The results of this study pave the way for further gene functional studies and genome editing in Chinese cabbage.

Copper toxicity in Chinese cabbage is not influenced by plant sulphur status, but affects sulphur metabolism-related gene expression and the suggested regulatory metabolites

Plant Biology ◽  
2013 ◽  
Vol 16 (1) ◽  
pp. 68-78 ◽  
Author(s):  
M. Shahbaz ◽  
C. E. E. Stuiver ◽  
F. S. Posthumus ◽  
S. Parmar ◽  
M. J. Hawkesford ◽  
...  
Keyword(s):  
Gene Expression ◽  
Chinese Cabbage ◽  
Copper Toxicity ◽  
Sulphur Metabolism ◽  
Related Gene

scholarly journals Resistance to Plasmodiophora brassicae in Brassica rapa and Brassica juncea genotypes From China

Plant Disease ◽  
2015 ◽  
Vol 99 (6) ◽  
pp. 776-779 ◽  
Author(s):  
Hui Zhang ◽  
Jie Feng ◽  
Shujiang Zhang ◽  
Shifan Zhang ◽  
Fei Li ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Significant Interaction ◽  
Plasmodiophora Brassicae ◽  
Inbred Lines ◽  
Vegetable Crops ◽  
Highly Pathogenic ◽  
Clubroot Disease ◽  
Pak Choi ◽  
China Development

Clubroot disease, caused by Plasmodiophora brassicae Woronin, has become a major problem in cruciferous crops worldwide. Chinese cabbage (Brassica rapa), pak choi (B. rapa), and mustard (B. juncea) are important vegetable crops in China. Development of clubroot-resistant cultivars of these crops is urgently needed. In this study, 71 B. rapa and B. juncea genotypes from China, including cultivars and inbred lines, were evaluated for resistance to three P. brassicae pathotypes. A significant interaction was observed between the P. brassicae pathotypes and the Brassica genotypes. Pathotype 3, as defined on the differentials of Williams, exhibited the weakest virulence on all plant material. By contrast, pathotypes 5 and 6 were both highly pathogenic on most of the tested genotypes. In all, 10 of the 14 Chinese cabbage cultivars were resistant to all three pathotypes, while 4 were resistant only to a specific pathotype. Seven of eight progenies obtained from the selfing of Chinese cabbage cultivars were resistant to pathotype 3 but most were susceptible to pathotypes 5 and 6. Most inbred lines of Chinese cabbage and all inbred lines of pak choi and mustard were susceptible to all three pathotypes but their susceptibility was lower to pathotype 3 than to pathotypes 5 and 6.

scholarly journals Genomic inferences of domestication events are corroborated by written records in Brassica rapa

2017 ◽  
Author(s):  
Xinshuai Qi ◽  
Hong An ◽  
Aaron Ragsdale ◽  
Tara E. Hall ◽  
Ryan N. Gutenkunst ◽  
...  
Keyword(s):  
Brassica Rapa ◽  
Chinese Cabbage ◽  
Morphological Diversity ◽  
Pak Choi ◽  
Demographic Models ◽  
Demographic Modeling ◽  
European Central ◽  
Genetic Groups ◽  
Central Asian ◽  
Genome Wide

AbstractDemographic modeling is often used with population genomic data to infer the relationships and ages among populations. However, relatively few analyses are able to validate these inferences with independent data. Here, we leverage written records that describe distinct Brassica rapa crops to corroborate demographic models of domestication. Brassica rapa crops are renowned for their outstanding morphological diversity, but the relationships and order of domestication remains unclear. We generated genome-wide SNPs from 126 accessions collected globally using high-throughput transcriptome data. Analyses of more than 31,000 SNPs across the B. rapa genome revealed evidence for five distinct genetic groups and supported a European-Central Asian origin of B. rapa crops. Our results supported the traditionally recognized South Asian and East Asian B. rapa groups with evidence that pak choi, Chinese cabbage, and yellow sarson are likely monophyletic groups. In contrast, the oil-type B. rapa subsp. oleifera and brown sarson were polyphyletic. We also found no evidence to support the contention that rapini is the wild type or the earliest domesticated subspecies of B. rapa. Demographic analyses suggested that B. rapa was introduced to Asia 2400-4100 years ago, and that Chinese cabbage originated 1200-2100 years ago via admixture of pak choi and European-Central Asian B. rapa. We also inferred significantly different levels of founder effect among the B. rapa subspecies. Written records from antiquity that document these crops are consistent with these inferences. The concordance between our age estimates of domestication events with historical records provides unique support for our demographic inferences.

Sign in / Sign up

Export Citation Format

Share Document