Phytochemical investigation and evaluation of in vitro free radical scavenging activity of Tabernaemontana divaricata Linn.

2010 ◽  
Vol 24 (3) ◽  
pp. 300-304 ◽  
Author(s):  
Sachin Jain ◽  
Avijeet Jain ◽  
Neetesh Jain ◽  
D.K. Jain ◽  
Neelam Balekar
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Tabernaemontana Divaricata ◽  
Phytochemical Investigation

scholarly journals Antioxidant & analgesic activities of leaves of Panlata (Derris trifoliate): In vitro investigation

2012 ◽  
Vol 1 (2) ◽  
Author(s):  
Md Raihan Sarkar ◽  
Moynul Hasan ◽  
Md Sariful Islam Howlader ◽  
Mohammad Saydur Rahman ◽  
Shubhra Kanti Dey
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Free Radical Scavenging ◽  
Free Radical Scavenging Activity ◽  
Scavenging Activity ◽  
Immersion Method ◽  
Standard Drug ◽  
In Vitro Investigation

In the present study, the antioxidant and analgesic potential of the ethanolic extract of the leaves of Derris trifoliata was evaluated. The free radical scavenging activity of the crude extract on the stable radical 1, 1-diphenyl-2-picrylhydrazyl (DPPH) was determined by comparing the DPPH inhibitory capacity of the extract. In the quantitative assay, Derris trifoliata extract displayed a free radical scavenging activity in the DPPH assay (IC50 = 19 ?g/ml) which is comparable to that of ascorbic acid (IC50 = 7.80 ?g/ml), a well-known standard antioxidant. The analgesic responses of the given samples of extracts were evaluated using the Tail immersion method. In the analgesic activity test, extract at dose of 200 mg/kg and 400 mg/kg exhibited significant (P<0.05 and P<0.001 respectively) inhibition of pain by 166.82 and 184.95 after 120 and 180 minutes respectively while the standard drug Diclofenac Na inhibition was found to be 217.67 after 180 minutes at a dose of 25 mg/kg body weight DOI: http://dx.doi.org/10.3329/ijpls.v1i2.12951 International Journal of Pharmaceutical and Life Sciences Vol.1(2) 2012

scholarly journals Preliminary Phytochemical Screening and In vitro Antioxidant Activity of Ethiopian Indigenous Medicinal Plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L.

2016 ◽  
Vol 8 (01) ◽  
Author(s):  
Suresh Kumar P. Nair ◽  
Kumar Ganesan ◽  
Henok Azalewor ◽  
Neethu Letha ◽  
Sharmila Gani
Keyword(s):  
Free Radical ◽  
Radical Scavenging Activity ◽  
Radical Scavenging ◽  
Natural Antioxidants ◽  
Free Radical Scavenging ◽  
Ocimum Basilicum ◽  
Scavenging Activity ◽  
Phytochemical Screening ◽  
Phytochemical Investigation

Antioxidants are the chemical substances which prevent the free radicals damage in the body. Numerous researches are going globally focussed on investigating natural antioxidants of plants origins. The aims of the present study were to evaluate preliminary phytochemical investigation and in vitro antioxidant activities of Ethiopian indigenous medicinal plants, Ocimum lamiifolium Hochst. ex Benth and Ocimum basilicum L. Aqueous, benzene and hexane crude leaves extracts of O. lamiifolium and O. basilicum were subjected to qualitative phytochemical screening using standard procedures. In addition, we investigated the antioxidant potential of crude aqueous leaves extract of O. lamiifolium and O. basilicum using tests involving inhibition of superoxide anions, DPPH, H2O2, NO and ABTS. Preliminary phytochemical investigation for benzene, hexane and aqueous extracts found alkaloids, sterols, carbohydrate and glycosides, tannins and flavonoids. The fraction inhibition of lipid peroxide at the first stage of oxidation illustrated antioxidant activity of O. lamiifolium and O. basilicum as 90% and 88% compared to those of gallic acid (97%) and BHT (84%) respectively. Also, the aqueous leaves extract of O. lamiifolium and O. basilicum exhibited significant DPPH free radical scavenging activity, nitric acid free radical scavenging activity assay, superoxide anion scavenging activity, ABTS scavenging activity and hydrogen peroxide free radical scavenging assay. Our findings provide confirmation that the aqueous leaves extract of O. lamiifolium and O. basilicum are potential source of natural antioxidants, and this warranted its uses in traditional medicine systems.

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