Effects of Whole Cigarette Smoke on Human Gingival Fibroblast Adhesion, Growth, and Migration

2011 ◽  
Vol 74 (13) ◽  
pp. 848-862 ◽  
Author(s):  
Abdelhabib Semlali ◽  
Jamila Chakir ◽  
Mahmoud Rouabhia
Keyword(s):  
Cigarette Smoke ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Fibroblast Adhesion ◽  
And Migration

scholarly journals Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts

2016 ◽  
Vol 5 (2) ◽  
pp. 83-88
Author(s):  
Thuy Anh Vu Pham ◽  
Hao TT Nguyen ◽  
My TN Nguyen ◽  
Van NL Trinh ◽  
Nga Y Tran ◽  
...  
Keyword(s):  
Wound Healing ◽  
Human Gingival Fibroblasts ◽  
Human Gingival Fibroblast ◽  
Complete Medium ◽  
Gingival Fibroblast ◽  
Gingival Fibroblasts ◽  
Platelet Rich Fibrin ◽  
Liquid Extract ◽  
And Migration ◽  
Proliferation And Migration

ABSTRACT Aims Our study focused on the fabrication of platelet-rich fibrin (PRF) and evaluated its influences on cell behaviors, including proliferation and migration. Materials and methods Platelet-rich fibrin was prepared from human peripheral blood according to Choukroun's method without using nonanticoagulant and foreign factors for platelet activation. Platelet-rich fibrin architecture was studied by hematoxylin and eosin staining. The investigation of PRF effects on human gingival fibroblasts (hGFs) was conducted via PRF liquid extract. Cell proliferation was determined via the number of cells after a period of time incubated in PRF liquid extract. Influence of PRF liquid extract on the migration of hGFs was conducted via scratch wound healing assay. Results Histological staining reviewed the natural fibrin fiber matrix of PRF. Platelet-rich fibrin liquid extract promoted hGF proliferation after 7 days of cultivation. Human gingival fibroblast proliferation in PRF liquid extract was more superior than those cultured in complete medium. Platelet-rich fibrin was also found to be able to promote the migration of hGFs for up to 48 hours. Conclusion These results indicated that PRF is suitable to be used as autologous natural biomaterial in supporting wound healing and in further application in periodontitis treatments. How to cite this article Nguyen HTT, Nguyen MTN, Trinh VNL, Tran NY, Ngo LTQ, Pham TAV, Tran HLB. Platelet-rich Fibrin Influences on Proliferation and Migration of Human Gingival Fibroblasts. Int J Experiment Dent Sci 2016;5(2):83-88.

scholarly journals Evaluation of the In Vitro Oral Wound Healing Effects of Pomegranate (Punica granatum) Rind Extract and Punicalagin, in Combination with Zn (II)

Biomolecules ◽  
2020 ◽  
Vol 10 (9) ◽  
pp. 1234 ◽  
Author(s):  
Vildan Celiksoy ◽  
Rachael L. Moses ◽  
Alastair J. Sloan ◽  
Ryan Moseley ◽  
Charles M. Heard
Keyword(s):  
Wound Healing ◽  
Antioxidant Activities ◽  
Punica Granatum ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Anti Inflammatory ◽  
High Concentrations ◽  
And Migration ◽  
Oral Wound Healing

Pomegranate (Punica granatum) is a well-established folklore medicine, demonstrating benefits in treating numerous conditions partly due to its antimicrobial and anti-inflammatory properties. Such desirable medicinal capabilities are attributed to a high hydrolysable tannin content, especially punicalagin. However, few studies have evaluated the abilities of pomegranate to promote oral healing, during situations such as periodontal disease or trauma. Therefore, this study evaluated the antioxidant and in vitro gingival wound healing effects of pomegranate rind extract (PRE) and punicalagin, alone and in combination with Zn (II). In vitro antioxidant activities were studied using DPPH and ABTS assays, with total PRE phenolic content measured by Folin–Ciocalteu assay. PRE, punicalagin and Zn (II) combination effects on human gingival fibroblast viability/proliferation and migration were investigated by MTT assay and scratch wounds, respectively. Punicalagin demonstrated superior antioxidant capacities to PRE, although Zn (II) exerted no additional influences. PRE, punicalagin and Zn (II) reduced gingival fibroblast viability and migration at high concentrations, but retained viability at lower concentrations without Zn (II). Fibroblast speed and distance travelled during migration were also enhanced by punicalagin with Zn (II) at low concentrations. Therefore, punicalagin in combination with Zn (II) may promote certain anti-inflammatory and fibroblast responses to aid oral healing.

scholarly journals Effect of Er:YAG Laser on Surface Properties, Bacterial and Lipopolysaccharide Clearance, and Human Gingival Fibroblast Adhesion: An In-Vitro Peri-Implantitis Model

2021 ◽  
Author(s):  
Cheng-Ying Wang ◽  
Bor-Shiunn Lee ◽  
Ya-Ting Jhang ◽  
Kevin Sheng-Kai Ma ◽  
Chen-Pang Huang ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Fluorescent Microscopy ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Significant Difference ◽  
Group 6 ◽  
Fibroblast Adhesion

Abstract Purpose: To investigate the effect of Er:YAG laser treatment on lipopolysaccharide (LPS) clearance and fibroblast adhesion in an ex vivo peri-implantitis model. Methods: Grade IV titanium discs (n = 216) were used and allocated to 6 groups. Group 1 was the negative control without Porphyromonas gingivalis inoculation. Discs in Groups 2 to 6 were incubated with P. gingivalis to form a biofilm. Group 3 received 0.12% chlorhexidine irrigation and Group 4 received titanium curettage to remove the biofilm. Group 5 was treated with Er:YAG laser irradiation and Group 6 was treated with titanium curettage plus Er:YAG laser irradiation. The contact angle and surface roughness were measured after the various treatments. The surface microstructure and residual bacteria were examined using scanning electron microscopy and confocal laser scanning microscopy, respectively. Residual LPS was examined using a limulus amoebocyte lysate assay and human gingival fibroblast adhesion was quantified using fluorescent microscopy. Results: Curettage plus Er:YAG laser irradiation was the most effective method for removing bacteria and LPS. No significant difference in the amount of fibroblast adhesion was found between the control and Group 6. Conclusion: Combined use of Er:YAG laser irradiation and curettage is a promising therapy for managing peri-implantitis .

scholarly journals Er:YAG laser irradiation enhances bacterial and lipopolysaccharide clearance and human gingival fibroblast adhesion on titanium discs

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Chen-Ying Wang ◽  
Bor-Shiunn Lee ◽  
Ya-Ting Jhang ◽  
Kevin Sheng-Kai Ma ◽  
Chen-Pang Huang ◽  
...  
Keyword(s):  
Laser Irradiation ◽  
Er:Yag Laser ◽  
Fluorescent Microscopy ◽  
Laser Scanning Microscopy ◽  
Confocal Laser ◽  
Human Gingival Fibroblast ◽  
Gingival Fibroblast ◽  
Significant Difference ◽  
Group 6 ◽  
Fibroblast Adhesion

AbstractTo investigate the effect of Er:YAG laser treatment on lipopolysaccharide (LPS) clearance and fibroblast adhesion on titanium disks. Grade IV titanium discs (n = 216) were used and allocated to 6 groups. Group 1 was the negative control without Porphyromonas gingivalis inoculation. Discs in Groups 2–6 were incubated with P. gingivalis to form a biofilm. Group 3 received 0.12% chlorhexidine irrigation and Group 4 received titanium curettage to remove the biofilm. Group 5 was treated with Er:YAG laser irradiation and Group 6 was treated with titanium curettage plus Er:YAG laser irradiation. The contact angle and surface roughness were measured after the various treatments. The surface microstructure and residual bacteria were examined using scanning electron microscopy and confocal laser scanning microscopy, respectively. Residual LPS was examined using a limulus amoebocyte lysate assay and human gingival fibroblast adhesion was quantified using fluorescent microscopy. Curettage plus Er:YAG laser irradiation was the most effective method for removing bacteria and LPS. No significant difference in the amount of fibroblast adhesion was found between the control and Group 6. Combined use of Er:YAG laser irradiation and curettage optimizes LPS clearance and fibroblast adhesion on titanium discs.

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