DEVELOPMENT OF QUANTITATIVE RECEPTOR-LIGAND BINDING ASSAY FOR USE AS A TOOL TO ESTIMATE IMMUNE RESPONSES AGAINSTPlasmodium vivaxDUFFY BINDING PROTEIN REGION II

2012 ◽  
Vol 33 (4) ◽  
pp. 403-413 ◽  
Author(s):  
Ahmad Rushdi Shakri ◽  
M. Moshahid A. Rizvi ◽  
Chetan E. Chitnis
Keyword(s):  
Immune Responses ◽  
Ligand Binding ◽  
Binding Assay ◽  
Binding Protein ◽  
Ligand Binding Assay ◽  
Receptor Ligand ◽  
Region Ii

Cell-based β2-adrenergic receptor–ligand binding assay using synthesized europium-labeled ligands and time-resolved fluorescence

2009 ◽  
Vol 392 (2) ◽  
pp. 103-109 ◽  
Author(s):  
Eija Martikkala ◽  
Mirva Lehmusto ◽  
Minna Lilja ◽  
Anita Rozwandowicz-Jansen ◽  
Jenni Lunden ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Adrenergic Receptor ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Time Resolved Fluorescence ◽  
Receptor Ligand ◽  
Time Resolved ◽  
Β2 Adrenergic Receptor

A Chemiluminescence Based Receptor-ligand Binding Assay Using Peptide Ligands with an Acridinium Ester Label

BIO-PROTOCOL ◽  
2015 ◽  
Vol 5 (6) ◽  
Author(s):  
Mari Wildhagen ◽  
Melinka Butenko ◽  
Reidunn Aalen ◽  
Georg Felix ◽  
Markus Albert
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Peptide Ligands ◽  
Ligand Binding Assay ◽  
Receptor Ligand ◽  
Acridinium Ester

scholarly journals HUMAN SERUM FOLATE CAN BE MEASURED USING FOLATE BINDING PROTEIN LINKED TO ENZYME-LABELED PROTEIN LIGAND BINDING ASSAY (ELPLBA) AS WELL AS ELISA

2019 ◽  
Vol 1 (2) ◽  
pp. 59-67
Author(s):  
Muhamad Arif Budiman ◽  
Mohamad Sadikin ◽  
Ani Retno Prijanti
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Binding Protein ◽  
Serum Folate ◽  
Assay Method ◽  
Enzyme Linked Immunosorbent Assay ◽  
Ligand Binding Assay ◽  
Elisa Method ◽  
Folate Binding Protein ◽  
Significant Difference

Background: Folate is an important substance used for purine and pyrimidine nucleotide synthesis. One measurement of folate that already establishes is using ELISA (Enzyme-linked immunosorbent assay) method. Folate binding protein is a protein that can bind folate, therefore it considered can be used as a tool that can replace antibody dependent ELISA method.Objectives: The aim of this research was to create a method for folate measurement in serum called Enzyme-labeled protein ligand binding assay (ELPLBA) by replacing antibody as used in ELISA method with folate binding protein (FBP) that purified from the whey of milk.Methods: The method is tested using 20 serum samples and compared to ELISA. Folate binding protein was purified from bovine’s milk using ammonium sulfate up to 90% saturated, DEAE-cellulose anion exchange chromatography and affinity chromatography. SDS-PAGE and western blot were used to establish the protein band of FBP that has molecular weight of ~25-35 kDa. ELPLBA was arranged with stationary phase using aminohexyl-agarose, and folic acid linked on it using carbodiimide.Results: The result show there was no significant difference of folate concentration between ELPLBA (14.804 ± 2.795) and ELISA method (13.859 ± 3.638), p = 0.363.Conclusion:  ELPLBA method show similarity for determination of folate in serum which was the same as standard folate measurement (ELISA).

Identification of a fibronectin-binding protein of Treponema lecithinolyticum by two-dimensional gel electrophoresis and ligand binding assay

2007 ◽  
Vol 53 (10) ◽  
pp. 1185-1190 ◽  
Author(s):  
Hae-Ri Lee ◽  
Bong-Kyu Choi
Keyword(s):  
Ligand Binding ◽  
Gel Electrophoresis ◽  
Binding Assay ◽  
Binding Protein ◽  
Bacterial Attachment ◽  
Host Cells ◽  
Ligand Binding Assay ◽  
Two Dimensional Gel Electrophoresis ◽  
Fibronectin Binding Protein ◽  
Fibronectin Binding

Treponema lecithinolyticum is associated with periodontitis and endodontic infections. As a critical early step in the infection process, fibronectin-binding protein (Fbp) is known to be involved in the adhesion of bacteria to cell surfaces for colonization and, hence, is considered to be a virulence factor. In this study, we identified an Fbp from the T. lecithinolyticum cell surface with a molecular mass of about 52 kDa by using 2-dimensional gel electrophoresis followed by a ligand binding assay. As T. lecithinolyticum is capable of binding to soluble and immobilized fibronectin, this Fbp may contribute to bacterial attachment to host cells.

scholarly journals Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay

2005 ◽  
Vol 10 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Marko E. Tirri ◽  
Roope J. Huttunen ◽  
Juha Toivonen ◽  
Pirkko L. Härkönen ◽  
Juhani T. Soini ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Fluorescence Polarization ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Receptor Ligand ◽  
Lead Compounds ◽  
Two Photon ◽  
Photon Excitation ◽  
Homogeneous Assay ◽  
Estrogen Receptor Ligand

Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

TBG MEASUREMENTS BY COMPETITIVE LIGAND BINDING ASSAY (CLBA)

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S15
Author(s):  
K. H. Rudorff ◽  
H. J. Kröll ◽  
J. Herrmann
Keyword(s):  
Ligand Binding ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Competitive Ligand
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