Contribution of material properties of cellular components on the viscoelastic, stress-relaxation response of a cell during AFM indentation

Author(s):  
Ginu U. Unnikrishnan ◽  
Vinu U. Unnikrishnan ◽  
J. N. Reddy
Author(s):  
M.Z. Butt ◽  
Samee Ullah ◽  
M. Rashid Khan ◽  
Sajjad Ahmad ◽  
Syed Zafar Ilyas

2017 ◽  
Author(s):  
Henry Heberle ◽  
Marcelo Falsarella Carazzolle ◽  
Guilherme P. Telles ◽  
Gabriela Vaz Meirelles ◽  
Rosane Minghim

AbstractBackgroundThe advent of “omics” science has brought new perspectives in contemporary biology through the high-throughput analyses of molecular interactions, providing new clues in protein/gene function and in the organization of biological pathways. Biomolecular interaction networks, or graphs, are simple abstract representations where the components of a cell (e.g. proteins, metabolites etc.) are represented by nodes and their interactions are represented by edges. An appropriate visualization of data is crucial for understanding such networks, since pathways are related to functions that occur in specific regions of the cell. The force-directed layout is an important and widely used technique to draw networks according to their topologies. Placing the networks into cellular compartments helps to quickly identify where network elements are located and, more specifically, concentrated. Currently, only a few tools provide the capability of visually organizing networks by cellular compartments. Most of them cannot handle large and dense networks. Even for small networks with hundreds of nodes the available tools are not able to reposition the network while the user is interacting, limiting the visual exploration capability.ResultsHere we propose CellNetVis, a web tool to easily display biological networks in a cell diagram employing a constrained force-directed layout algorithm. The tool is freely available and open-source. It was originally designed for networks generated by the Integrated Interactome System and can be used with networks from others databases, like InnateDB.ConclusionsCellNetVis has demonstrated to be applicable for dynamic investigation of complex networks over a consistent representation of a cell on the Web, with capabilities not matched elsewhere.


Author(s):  
Raghu V. Prakash ◽  
Monalisha Maharana

Polymer composites have a characteristic, composition specific visco-elastic property which influences the damage progression during fatigue cycling. While some researchers have studied the time dependent constitutive response of polymer composites during the first cycle of fatigue loading, very few have experimentally investigated the dependence of visco-elastic response of built-up polymer composite materials at various stages of fatigue cycling [1]. Our earlier studies on fatigue response of polymer composites focused primarily on the stiffness degradation as a function of applied cycles of loading, which represents the gross response of the material [2]. While doing such an experiment, complimentary experimental techniques to measure the temperature evolution was attempted through the use of infrared thermal imaging technique, which gave some insight into the change in temperature response as a function of fatigue cycling. However, there was no systematic measurement of creep and stress relaxation response of the composite material as a function of induced fatigue damage. The present paper describes the results of creep and stress-relaxation obtained during uni-axial fatigue loading of a hybrid polymer composite material. For this purpose, a woven carbon fiber mat was chosen as the synthetic fiber and Flax fiber in the unidirectional form was chosen as the natural fiber that is laid between the two layers of woven carbon fiber mat. Epoxy LY 556 and hardener Araldite® was used for building up of composite laminate by hand-lay-up technique. Dog-bone shaped tensile specimens with a gage width of 13 mm and gage length of 57 mm were extracted from the 250 × 250 mm sq. plate laminate of 2.1 mm thickness using a numerical controlled milling machine. The specimens were tested at 35% of their median tensile strengths under fatigue at a positive stress ratio (Pmin/Pmax) of 0.1 in tension-tension loading. Prior to start of fatigue loading, the specimens were held in load control and the strain in the gage length was measured for understanding the creep response over 2500 seconds. For stress-relaxation characterization, the specimens were held in extensometer control over a period of 2500 sec. The creep and stress relaxation tests were carried out after periodic intervals of fatigue cycling. It was observed that in the case of un-impacted specimens, the creep rate is consistent with the stiffness variation, which in turn, is dependent on the number of fatigue cycles - till it showed signs of de-lamination. Thereafter it was governed by the woven synthetic fiber response. Similarly, the stress relaxation response was found to decrease with increasing fatigue cycles. In case of impacted specimens, the local deformation had a prominent role in terms of creep and stress relaxation response.


2021 ◽  
Vol 7 (2) ◽  
pp. 363-366
Author(s):  
Thomas Reuter ◽  
Christof Hurschler

Abstract Mechanical parameters of hard and soft tissues are explicit markers for quantitative tissue characterization. In this study, we present a comparison of biphasic material properties of equine articular cartilage estimated from stress relaxation (ε = 6 %, t = 1000 s) and creep indentation tests (F = 0.1 N, t = 1000 s). A biphasic 3D-FE-based method is used to determine the biomechanical properties of equine articular cartilage. The FE-model computation was optimized by exploiting the axial symmetry and mesh resolution. Parameter identification was executed with the Levenberg- Marquardt-algorithm. Additionally, sensitivity analyses of the calculated biomechanical parameters were performed. Results show that the Young’s modulus E has the largest influence and the Poisson’s ratio of ν ≤ 0.1 is rather insensitive. The R² of the fit results varies between 0.882 and 0.974 (creep model) and between 0.695 and 0.930 (relaxation model). The averaged parameters E and k determined from the creep model yield higher values in comparison to the relaxation model. The differences can be traced back to the experimental settings and to the biphasic material model.


Geophysics ◽  
2022 ◽  
pp. 1-56
Author(s):  
Ankush Singh ◽  
Mark D. Zoback

Knowledge of layer-to-layer variations of the least principal stress, S hmin, with depth is essential for optimization of multi-stage hydraulic fracturing in unconventional reservoirs. Utilizing a geomechanical model based on viscoelastic stress relaxation in relatively clay rich rocks, we present a new method for predicting continuous S hmin variations with depth. The method utilizes geophysical log data and S hmin measurements from routine diagnostic fracture injection tests (DFITs) at several depths for calibration. We consider a case study in the Wolfcamp formation in the Midland Basin, where both geophysical logs and values of S hmin from DFITs are available. We compute a continuous stress profile as a function of the well logs that fits all of the DFITs well. We utilized several machine learning technologies, such as bootstrap aggregation (or bagging), to improve the generalization of the model and demonstrate that the excellent fit between predicted and observed stress values is not the result of over-fitting the calibration points. The model is then validated by accurately predicting hold-out stress measurements from four wells within the study area and, without recalibration, accurately predicting stress as a function of depth in an offset pad about 6 miles away.


2018 ◽  
Vol 2018 (HiTEC) ◽  
pp. 000148-000153
Author(s):  
Kenneth P. Dowhower

Abstract The electrical interconnect is an essential component of most electrical system configurations. The ability of the interconnect interface to reliably transmit power and / or data throughout the system is critical to its overall performance. Degradation of the mechanical or electrical properties of the interface can reduce the system performance or in severe cases, make it inoperable. There are several factors which can inhibit the performance of the interconnect, one of most severe is long term exposure to elevated temperatures. This effect can also be accelerated when combined with other severe environmental conditions such as high vibration and physical shock, which are often found in down hole oil and gas well drilling applications. This type of exposure can significantly degrade the essential properties of a reliable electrical interface such as contact resistance, mechanical stability, and electrical isolation. This paper will present options for design features and material properties that can be incorporated into an interconnect design that will mitigate these adverse effects. Specifically, this paper addresses the material properties of the contact interface and its surface treatment, the mechanical and electrical properties of the insulating material, the robustness of the mating features and the contact retention system. Two key features of the contact interface that are discussed are the stability of its electrical resistance and the robustness of its mechanical retention. Long term exposure to high temperatures typically induces stress relaxation in the compliant members of the contact interface that are required to produce a stable, low resistance interface, while allowing for a high level of mate / unmate durability. Stress relaxation can also reduce the mechanical stability of the contact interface where metal or plastic retention features are utilized. In the case of retention through epoxy bonding, imparting thermal stress at the bonding surface can result in loss of adhesion and / or retention. The surface treatment of the contact interface has also been shown to be a contributing factor in its electrical stability in high temperature applications. Typically, the interface is plated with a hard gold over nickel finish, which provides a noble interface that is corrosion resistant, but with the hardness required to withstand many mate / unmate cycles. A small percentage of nickel or cobalt are typically alloyed with the gold to produce the required hardness. In most applications, it has minimal impact on the overall resistance of the contact interface. In high temperature applications, however, it can tend to diffuse through the gold to the contact interface. Since these materials have a higher resistivity, they can negatively affect the resistance of the interface. The impact of this effect is reviewed in this paper. Finally, results of the evaluations on high temperature insulating materials and bonding epoxies are presented in this paper. The mechanical and dielectric stability of the insulating materials and the adhesion properties of the epoxy used for contact retention were the primary concerns for their evaluation. The verification tests that included at temperature exposure were conducted at +260°C to simulate extreme use cases for most down hole applications.


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