Abstract. A 1342 yr-long tree-ring chronology was developed from Qilian junipers in the central Qilian Mountains of the north-eastern Tibetan Plateau, China. The climatic implications of this chronology were investigated using simple correlation, partial correlation and response function analyses. The chronology was significantly positively correlated with temperature variables during the pre- and current growing seasons, especially with minimum temperature. The variability of the mean minimum temperature from January to August since 670 AD was then reconstructed based on the tree-ring chronology. The reconstruction explained 58.5% of the variance in the instrumental temperature records during the calibration period (1960–2011) and captured the variation patterns in minimum temperature at the annual to centennial time scales over the past millennium. The most recent 50 yr were the warmest period, while 1690–1880 was the coldest period since 670 AD. Comparisons with other temperature series from neighbouring regions and for the Northern Hemisphere as a whole supported the validity of our reconstruction and suggested that it provided a good regional representation of temperature change in the north-eastern Tibetan Plateau. The results of multi-taper spectral analysis showed the occurrence of significant quasi-periodic behaviour at a number of periods (2–3, 28.8–66.2, 113.6–169.5, and 500 yr), which were consistent with those associated with El Niño-Southern Oscillation (ENSO), Pacific Decadal Oscillation (PDO) and solar activity. Some reconstructed cold events may have close relationship with the volcanic eruptions.