Multiple swallow behaviour during high resolution pharyngeal manometry: prevalence and sub-typing in healthy adults

Abstract We report the incidence of electrophoretically homogeneous protein bands occurring in the gamma-globulin region in serum samples from 600 healthy adults, as determined with a high-resolution electrophoretic system. One or more electrophoretically homogeneous protein bands of various intensities were detected in the gamma-globulin region of 30 specimens when we used an improved agarose electrophoretic system, and in two specimens when we used a cellulose acetate electrophoretic system. Improvement of the agarose gel electrophoretic system thus enhanced its sensitivity in defining homogeneous protein bands in the gamma-globulin region. The incidence of these bands in a healthy population is approximately 5%.

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Normative reference values for esophageal high‐resolution manometry in healthy adults: A systematic review

Neurogastroenterology & Motility ◽

10.1111/nmo.13954 ◽

2020 ◽

Vol 33 (1) ◽

Cited By ~ 1

Author(s):

Luis G. Alcala Gonzalez ◽

Renske A. B. Oude Nijhuis ◽

Andreas J. P. M. Smout ◽

Albert J. Bredenoord

Keyword(s):

Systematic Review ◽

High Resolution ◽

Reference Values ◽

Healthy Adults ◽

High Resolution Manometry

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Effect of topical nasal anesthetic on swallowing in healthy adults: A double‐blind, high‐resolution manometry study

The Laryngoscope ◽

10.1002/lary.26996 ◽

2017 ◽

Vol 128 (6) ◽

pp. 1335-1339 ◽

Cited By ~ 8

Author(s):

Esther Guiu Hernandez ◽

Kristin Gozdzikowska ◽

Olivia Apperley ◽

Maggie‐Lee Huckabee

Keyword(s):

High Resolution ◽

Healthy Adults ◽

Double Blind ◽

High Resolution Manometry

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Evaluation of a commercial liquid-chromatography high-resolution mass-spectrometry method for the determination of hepcidin-25

Biochemia Medica ◽

10.11613/bm.2019.020701 ◽

2019 ◽

Vol 29 (2) ◽

pp. 292-301

Author(s):

Dietmar Enko ◽

Sieglinde Zelzer ◽

Günter Fauler ◽

Markus Herrmann

Keyword(s):

Mass Spectrometry ◽

Liquid Chromatography ◽

High Resolution ◽

High Resolution Mass Spectrometry ◽

Healthy Adults ◽

Serum Samples ◽

Acceptance Criteria ◽

Reliable Determination ◽

Freeze And Thaw

Introduction: Reliable determination of hepcidin-25, a key regulator of iron metabolism, is important. This study aimed at evaluating the performance of the Hepcidin-25 Liquid Chromatography-Tandem Mass-Spectrometry (LC-MS/MS) Kit (Immundiagnostik AG, Bensheim, Germany) for quantification of the hepcidin-25 protein. Materials and methods: Precision, accuracy, linearity, and preanalytical requirements of the liquid-chromatography high-resolution massspectrometry (LC-HR-MS) method were evaluated. The imprecision and bias acceptance criteria were defined ≤ 15%. We investigated sample stability at room temperature (RT) and after repeated freeze and thaw cycles. Additionally, we assessed serum hepcidin-25 concentrations of 165 healthy adults referred for a medical check-up. Results: The hepcidin-25 LC-MS/MS assay was linear over the concentration range of 3 – 200 ng/mL. Within- and between-run precision ranged between 1.9 – 8.6% and 5.1 – 12.4%, respectively. The mean bias of the low and high control material was - 2.7% and 2.1%, respectively. At RT, serum samples were stable for 3 h (mean bias + 0.3%). After two and three freeze and thaw cycles, hepcidin-25 concentrations showed a bias of +8.0 and + 20%, respectively. Of 165 healthy adults, 109 females had a significantly lower median of 8.42 (range: 1.00 – 60.10) ng/mL compared to 56 males with 15.76 (range: 1.50 – 60.50) ng/mL (P = 0.002). Conclusions: The hepcidin-25 LC-MS/MS kit shows a broad analytical range and meets the imprecision and bias acceptance criteria of ≤ 15%. Serum samples can be stored at RT for 3 h and resist up to two freeze and thaw cycles.

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Characterizing Swallows From People With Neurodegenerative Diseases Using High-Resolution Cervical Auscultation Signals and Temporal and Spatial Swallow Kinematic Measurements

Journal of Speech Language and Hearing Research ◽

10.1044/2021_jslhr-21-00134 ◽

2021 ◽

pp. 1-16

Author(s):

Cara Donohue ◽

Yassin Khalifa ◽

Shitong Mao ◽

Subashan Perera ◽

Ervin Sejdić ◽

...

Keyword(s):

Machine Learning ◽

High Resolution ◽

Neurodegenerative Diseases ◽

Learning Algorithms ◽

Healthy Adults ◽

Machine Learning Algorithms ◽

Cervical Auscultation ◽

Laryngeal Vestibule ◽

Temporal And Spatial ◽

Kinematic Measures

Purpose The prevalence of dysphagia in patients with neurodegenerative diseases (ND) is alarmingly high and frequently results in morbidity and accelerated mortality due to subsequent adverse events (e.g., aspiration pneumonia). Swallowing in patients with ND should be continuously monitored due to the progressive disease nature. Access to instrumental swallow evaluations can be challenging, and limited studies have quantified changes in temporal/spatial swallow kinematic measures in patients with ND. High-resolution cervical auscultation (HRCA), a dysphagia screening method, has accurately differentiated between safe and unsafe swallows, identified swallow kinematic events (e.g., laryngeal vestibule closure [LVC]), and classified swallows between healthy adults and patients with ND. This study aimed to (a) compare temporal/spatial swallow kinematic measures between patients with ND and healthy adults and (b) investigate HRCA's ability to annotate swallow kinematic events in patients with ND. We hypothesized there would be significant differences in temporal/spatial swallow measurements between groups and that HRCA would accurately annotate swallow kinematic events in patients with ND. Method Participants underwent videofluoroscopic swallowing studies with concurrent HRCA. We used linear mixed models to compare temporal/spatial swallow measurements ( n = 170 ND patient swallows, n = 171 healthy adult swallows) and deep learning machine-learning algorithms to annotate specific temporal and spatial kinematic events in swallows from patients with ND. Results Differences ( p < .05) were found between groups for several temporal and spatial swallow kinematic measures. HRCA signal features were used as input to machine-learning algorithms and annotated upper esophageal sphincter (UES) opening, UES closure, LVC, laryngeal vestibule reopening, and hyoid bone displacement with 66.25%, 85%, 68.18%, 70.45%, and 44.6% accuracy, respectively, compared to human judges' measurements. Conclusion This study demonstrates HRCA's potential in characterizing swallow function in patients with ND and other patient populations.

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The Utility of High-Resolution Pharyngeal Manometry in Dysphagia Treatment

Perspectives of the ASHA Special Interest Groups ◽

10.1044/2019_pers-sig13-2018-0027 ◽

2019 ◽

Vol 4 (3) ◽

pp. 507-516 ◽

Cited By ~ 1

Author(s):

Kate (Humphries) Davidson ◽

Ashli K. O'Rourke

Keyword(s):

High Resolution ◽

Oropharyngeal Dysphagia ◽

Targeted Interventions ◽

Competency Training ◽

Speech Language Pathologist ◽

Dose Planning ◽

Efficacy Assessment ◽

Strategy Planning ◽

Pharyngeal Manometry ◽

Dysphagia Treatment

Purpose High-resolution pharyngeal manometry (HRPM) is an emerging technology that shows promise as both an adjuvant diagnostic and therapeutic tool in oropharyngeal dysphagia management. Advances in manometric technology, including increased number of sensors and topographical pressure plots, enhance the biofeedback potential for the pharynx. This clinical focus article serves as an overview of the utility of HRPM in dysphagia treatment. Conclusion HRPM-facilitated biofeedback aids the patient in the correct implementation of clinical recommendations and also provides the clinician an assessment of the effectiveness and accuracy of those targeted interventions. Topographic pressure plots provide intuitive feedback, allow easier swallow-to-swallow comparisons, and produce visually color-coded pressure information for the patient and clinician in real time. Paired with existing, evidence-based interventions, HRPM biofeedback may facilitate maneuver and strategy planning, exercise training and monitoring, temporal coordination, upper esophageal segment relaxation and duration, swallow mapping (topographic pattern recognition and approximation), fatigue monitoring, dose planning, adherence tracking, and efficacy assessment of selected interventions. Although competency training is needed to effectively utilize HRPM, there are growing opportunities for the speech-language pathologist to acquire and implement this technology for the benefit of patients.

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