scholarly journals Daphne mucronata enhances cell proliferation and protects human adipose stem cells against monosodium iodoacetate induced oxidative stress in vitro

Adipocyte ◽  
2020 ◽  
Vol 9 (1) ◽  
pp. 495-508 ◽  
Author(s):  
Numan Fazal ◽  
Hamzah Khawaja ◽  
Nadia Naseer ◽  
Azim Jahangir Khan ◽  
Noreen Latief
Keyword(s):  
Oxidative Stress ◽  
Stem Cells ◽  
Cell Proliferation ◽  
Adipose Stem Cells ◽  
Monosodium Iodoacetate ◽  
Daphne Mucronata ◽  
Human Adipose Stem Cells

scholarly journals Modulation of Human Adipose Stem Cells’ Neurotrophic Capacity Using a Variety of Growth Factors for Neural Tissue Engineering Applications: Axonal Growth, Transcriptional, and Phosphoproteomic Analyses In Vitro

Cells ◽  
2020 ◽  
Vol 9 (9) ◽  
pp. 1939
Author(s):  
Katharina M. Prautsch ◽  
Alexander Schmidt ◽  
Viola Paradiso ◽  
Dirk J. Schaefer ◽  
Raphael Guzman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Growth Factors ◽  
Neurotrophic Factor ◽  
Axonal Growth ◽  
Cellular Level ◽  
Adipose Stem Cells ◽  
Transcriptional Analysis ◽  
Axonal Outgrowth ◽  
Human Adipose Stem Cells

We report on a potential strategy involving the exogenous neurotrophic factors (NTF) for enhancing the neurotrophic capacity of human adipose stem cells (ASC) in vitro. For this, ASC were stimulated for three days using NTF, i.e., nerve growth factor (NGF), brain-derived neurotrophic factor (BDNF), neurotrophin 3 (NT3), NT4, glial cell-derived neurotrophic factor (GDNF), and ciliary neurotrophic factor (CNTF). The resulting conditioned medium (CM) as well as individual NTF exhibited distinct effects on axonal outgrowth from dorsal root ganglion (DRG) explants. In particular, CM derived from NT3-stimulated ASC (CM-NT3-ASC) promoted robust axonal outgrowth. Subsequent transcriptional analysis of DRG cultures in response to CM-NT3-ASC displayed significant upregulation of STAT-3 and GAP-43. In addition, phosphoproteomic analysis of NT3-stimulated ASC revealed significant changes in the phosphorylation state of different proteins that are involved in cytokine release, growth factors signaling, stem cell maintenance, and differentiation. Furthermore, DRG cultures treated with CM-NT3-ASC exhibited significant changes in the phosphorylation levels of proteins involved in tubulin and actin cytoskeletal pathways, which are crucial for axonal growth and elongation. Thus, the results obtained at the transcriptional, proteomic, and cellular level reveal significant changes in the neurotrophic capacity of ASC following NT3 stimulation and provide new options for improving the axonal growth-promoting potential of ASC in vitro.

scholarly journals Effects of different serum conditions on osteogenic differentiation of human adipose stem cells in vitro

2013 ◽  
Vol 4 (1) ◽  
pp. 17 ◽  
Author(s):  
Laura Kyllönen ◽  
Suvi Haimi ◽  
Bettina Mannerström ◽  
Heini Huhtala ◽  
Kristiina M Rajala ◽  
...  
Keyword(s):  
Stem Cells ◽  
Osteogenic Differentiation ◽  
Adipose Stem Cells ◽  
Human Adipose Stem Cells

scholarly journals SIV Infection and the HIV Proteins Tat and Nef Induce Senescence in Adipose Tissue and Human Adipose Stem Cells, Resulting in Adipocyte Dysfunction

Cells ◽  
2020 ◽  
Vol 9 (4) ◽  
pp. 854 ◽  
Author(s):  
Jennifer Gorwood ◽  
Tina Ejlalmanesh ◽  
Christine Bourgeois ◽  
Matthieu Mantecon ◽  
Cindy Rose ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Insulin Resistance ◽  
Stem Cells ◽  
Adipose Tissue ◽  
Viral Proteins ◽  
Adipose Stem Cells ◽  
Siv Infection ◽  
P53 Activation ◽  
Human Adipose Stem Cells ◽  
P16 Expression

Background: Aging is characterized by adipose tissue senescence, inflammation, and fibrosis, with trunk fat accumulation. Aging HIV-infected patients have a higher risk of trunk fat accumulation than uninfected individuals—suggesting that viral infection has a role in adipose tissue aging. We previously demonstrated that HIV/SIV infection and the Tat and Nef viral proteins were responsible for adipose tissue fibrosis and impaired adipogenesis. We hypothesized that SIV/HIV infection and viral proteins could induce adipose tissue senescence and thus lead to adipocyte dysfunctions. Methods: Features of tissue senescence were evaluated in subcutaneous and visceral adipose tissues of SIV-infected macaques and in human adipose stem cells (ASCs) exposed to Tat or Nef for up to 30 days. Results: p16 expression and p53 activation were higher in adipose tissue of SIV-infected macaques than in control macaques, indicating adipose tissue senescence. Tat and Nef induced higher senescence in ASCs, characterized by higher levels of senescence-associated beta-galactosidase activity, p16 expression, and p53 activation vs. control cells. Treatment with Tat and Nef also induced oxidative stress and mitochondrial dysfunction. Prevention of oxidative stress (using N-acetyl-cysteine) reduced senescence in ASCs. Adipocytes having differentiated from Nef-treated ASCs displayed alterations in adipogenesis with lower levels of triglyceride accumulation and adipocyte marker expression and secretion, and insulin resistance. Conclusion: HIV/SIV promotes adipose tissue senescence, which in turn may alter adipocyte function and contribute to insulin resistance.

Buccal Fat Pad, an Oral Access Source of Human Adipose Stem Cells with Potential for Osteochondral Tissue Engineering: An In Vitro Study

2010 ◽  
Vol 16 (5) ◽  
pp. 1083-1094 ◽  
Author(s):  
Elisabet Farré-Guasch ◽  
Carles Martí-Pagès ◽  
Federico Hernández-Alfaro ◽  
Jenneke Klein-Nulend ◽  
Núria Casals
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
In Vitro Study ◽  
Adipose Stem Cells ◽  
Vitro Study ◽  
Fat Pad ◽  
Buccal Fat Pad ◽  
Osteochondral Tissue Engineering ◽  
Human Adipose Stem Cells

scholarly journals Enhanced Osteogenic and Vasculogenic Differentiation Potential of Human Adipose Stem Cells on Biphasic Calcium Phosphate Scaffolds in Fibrin Gels

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Fransisca A. S. van Esterik ◽  
Behrouz Zandieh-Doulabi ◽  
Cornelis J. Kleverlaan ◽  
Jenneke Klein-Nulend
Keyword(s):  
Stem Cells ◽  
Calcium Phosphate ◽  
Biphasic Calcium Phosphate ◽  
Adipose Stem Cells ◽  
Differentiation Potential ◽  
Fibrin Gels ◽  
Enhanced Expression ◽  
Human Adipose Stem Cells

For bone tissue engineering synthetic biphasic calcium phosphate (BCP) with a hydroxyapatite/β-tricalcium phosphate (HA/β-TCP) ratio of 60/40 (BCP60/40) is successfully clinically applied, but the high percentage of HA may hamper efficient scaffold remodelling. Whether BCP with a lower HA/β-TCP ratio (BCP20/80) is more desirable is still unclear. Vascular development is needed before osteogenesis can occur. We aimed to test the osteogenic and/or vasculogenic differentiation potential as well as degradation of composites consisting of human adipose stem cells (ASCs) seeded on BCP60/40 or BCP20/80 incorporated in fibrin gels that trigger neovascularization for bone regeneration. ASC attachment to BCP60/40 and BCP20/80 within 30 min was similar (>93%). After 11 days of culture BCP20/80-based composites showed increased alkaline phosphatase activity andDMP1gene expression, but notRUNX2and osteonectin expression, compared to BCP60/40-based composites. BCP20/80-based composites also showed enhanced expression of the vasculogenic markersCD31andVEGF189, but notVEGF165and endothelin-1. Collagen-1 and collagen-3 expression was similar in both composites. Fibrin degradation was increased in BCP20/80-based composites at day 7. In conclusion, BCP20/80-based composites showed enhanced osteogenic and vasculogenic differentiation potential compared to BCP60/40-based compositesin vitro, suggesting that BCP20/80-based composites might be more promising forin vivobone augmentation than BCP60/40-based composites.

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