Sunlight mediated rapid synthesis of small size range silver nanoparticles using Zingiber officinale rhizome extract and its antibacterial activity analysis

2018 ◽  
Vol 48 (2) ◽  
pp. 139-145 ◽  
Author(s):  
Shiji Mathew ◽  
Anagha Prakash ◽  
E. K. Radhakrishnan
2017 ◽  
Vol 19 (4) ◽  
pp. 128-136 ◽  
Author(s):  
Saviour A. Umoren ◽  
Alexis M. Nzila ◽  
Saravanan Sankaran ◽  
Moses M. Solomon ◽  
Peace S. Umoren

Abstract Silver nanoparticles (AgNPs) have been synthesized in the presence of Strawberry fruit extract (SBFE) at room temperature. The synthesized AgNPs was characterized by UV-vis spectroscopy, SEM, EDS, XRD, TEM and FTIR. The UV-vis spectra of the AgNPs show SPR band at 450 nm. TEM results indicate that AgNPs are spherical in shape and size range between 7–65 nm. Antibacterial activity of the synthesized AgNPs has been assessed against Pseudomonas aeruginosa and Bacillus licheniformis. The results show that AgNPs exhibit inhibitory effect and effect is a function of AgNPs concentration. The antibacterial activity of the prepared AgNPs has been compared with two antibiotics, amoxicillin and ciprofloxacin. It is found that the antibiotics perform better than AgNPs.


Author(s):  
Ho Thi Phuong ◽  
Nguyen Thi Le Na ◽  
Nguyen Trung Thanh ◽  
Nguyen Dinh Thang

Recently, using plant extract as a reducing agent for nanosilver particle synthesis hasbeen focused. This is a green technology utilizing the ready material in the nature to create thenanoparticles with good properties and uniqe quality. In this study, ginger rhizome extract wasused to reduce the silver cation (Ag + ) to silver (Ag o ) as nanoparticles with uniqe quality and evendistribution in the solution. The size of the particles varied in the range of 20-40 nm. Reactionconditions were investigated and optimized with AgNO 3 concentration of 3mM, extractsolution/AgNO 3 solution of 1/5, temperature of 80˚C, pH of 12 and reaction time of 30 min. Theresults obtained from the antibacterial assays showed that silver nanoparticle solution hadantibacterial ability with an average effective diameter of 10 mm. It also indicated that theantibacterial activity of silver nanoparticle solution on the Gram (-) bacterium (E. coli) is betterthat on Gram (+) bacterium (S. aureus). In conclusion, we suggest that the ginger rhizome extractcan be used to produce silver nanoparticles in mild reaction conditions; the silver nanoparticlesolution expressed as a quite good antibacterial agent and therefore could be applied in decreasingthe effects of deleterious bacteria.Keywords Silver nanoparticle, plant extract, antibacterial, Zingiber officinale Rosc. References [1] L.S. Li, J. Hu, W. Yang and P. Alivisatos. Band gap variation of size- and shape-controlled colloidal CdSe quantum rods. Nano Lett. 1(2001) 49-51. https://doi.org/10.1021/nl015559r.[2] A. P. Nikalje. Nanotechnology and its Applications in Medicine. Medicinal chemistry, 5(2015) 81-89.[3] G. Doria, J. Conde, B. Veigas et al. Noble metal nanoparticles for biosensing applications. Sensors 12(2012) 1657–1687. https://doi.org/ 10.4172/2161 -0444.1000247[4] A. J. Haes, A. D. McFarlan, R. P. van Duyne. Nanoparticle optics: sensing with nanoparticle arrays and single nanoparticles. The International Society for Optical Engineering 5223 (2003) 197–207. https://doi.org/10.1039/C7NR03311G.[5] A. Elham, M. Morteza, F. V. Sedigheh, K. Mohammad, A. Abolfazl, T. N. Hamid, N. Parisa, W. J. San, H. Younes, N-K. Kazem, S. Mohammad. Silver nanoparticcles: Synthesis methods, bio-applications and properties. Critical reviews in Microbiology 42(2016) 173-180. https://doi.org/10.3109/1040841X.2014.912200.[6] J. K. Pradeep, K. Chaudhury, V. S. Suresh, K. G. Sujoy. An emerging interface between life science and nanotechnology: present status and prospects of reproductive healthcare aided by nano-biotechnology. Nano Rev. 5(2014): 10.3402/ nano. v5. 22762. https://doi.org/10.3402/nano.v5.22762.[7] M. Danilcauk, A. Lund, J. Saldo, H. Yamada, J. Michalik. Conduction electron spin resonance of small silver particles. Spectrochimaca. Acta. Part A 63(2006) 189–191. https://doi.org/10.1016/j.saa. 2005.05.002[8] J. L. Elechiguerra, J. L. Burt, J. R. Morones et al. Interaction of silver nanoparticles with HIV-1. Journal of Nanobiotechnology 3(2005) 6. https:// doi.org/10.1186/1477-3155-3-6[9] J. S. Kim, E. Kuk, K. Yu, J. H. Kim, S. J. Park, H. J. Lee, S. H. Kim, Y. K. Park, Y. H. Park, C. Y. Hwang, Y. K. Kim, Y. S. Lee, D. H. Jeong, M. H. Cho. Antimicrobial effects of silver nanoparticles. Nanomedicine 3(2007) 95–101. https://doi.org/ 10.1016/j.nano.2006.12.001.[10] Y. Matsumura, K. Yoshikata, S. Kunisaki and T. Tsuchido. Mode of bacterial action of silver zeolite and its comparison with that of silver nitrate. Appl. Environ. Microbiol. 69(2003) 4278–4281.https://doi.org/10.1128/AEM.69.7.4278-4281. 2003.[11] M. Yamanaka, K. Hara, J. Kudo. Bactericidal Actions of a Silver Ion Solution on Escherichia coli, Studied by Energy-Filtering Transmission Electron Microscopy and Proteomic Analysis. Appl. Environ. Microbiol. 71(2005) 7589–7593. https://doi.org/10.1128/AEM.71.11.7589-7593. 2005.[12] Y. H. Hsueh, K. S. Lin, W. J. Ke, C. T. Hsieh, C. L. Chiang, D. Y. Tzou and S. T. Liu. The Antimicrobial Properties of Silver Nanoparticles in Bacillus subtilis Are Mediated by Released Ag+ Ions. PLoS One 10(2015):e0144306. https://doi.org/10.1371/journal.pone.0144306.[13] N. Kumar, S. Das, A. Jyoti and S. Kaushik. Synergistic effect of silver nanoparticles with doxycycline against Klebsiella pneumoniae. Int. J. Pharm. Sci. 8(2016) 183-186.[14] V. G. Borodina, Y. A. Mirgorod. Kinetics and Mechanism of Interaction between HAuCl4 and Rutin. Kinet. Cat. 55(2014) 683–687. https://doi. org/10.1134/S0023158414060044.[15] V. V. Makarov, A. J. Love, O. V. Sinitsyna, S. S. Makarova, I. V. Yaminsky, M. E. Taliansky, N. O. Kalinina. Green nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae 6(2014) 35–44. https://doi.org/10.1039/C1GC15 386B. [16] M. S. Butt, M. T. Sultan. Ginger and its health claims: molecular aspects. Critical Reviews in Food Science and Nutrition 51(2011) 383–393. https://doi.org/10.1080/10408391003624848[17] M. Park, J. Bae, D. S. Lee. Antibacterial activity of gingerol and gingerol isolated from ginger rhizome against periodontal bacterial. Phytotherapy Research 22(2008) 1446–1449. https://doi.org/10.1002/ptr.2473[18] Y. Shukla, M. Singh. Cancer preventive properties of ginger: a brief review. Food and Chemical Toxicology 45(2007) 683–690. https://doi.org/10. 1016/j.fct.2006.11.002.  


2020 ◽  
Vol 36 (4) ◽  
pp. 87-93
Author(s):  
V.Yu. Reshetova ◽  
A.F. Krivoshchepov ◽  
I.A. Butorova ◽  
N.B. Feldman ◽  
S.V. Lutsenko ◽  
...  

Chitosan beads with colloidal silver nanoparticles inclued in the polymer matrix have been obtained by the introduction of chitosan into an acidified nanosilver sol. Dual interconnection of drops of the resulting solution was then carried out by ionotropic gelation at the first stage and covalent crosslinking of the polymer matrix with adipic acid at the second stage. The surface morphology of the obtained beads was studied by scanning electron microscopy. Data of Fourier transform IR spectroscopy confirmed the formation of covalent bonds between chitosan and adipic acid. The antibacterial activity of obtained beads against S. aureus and E. coli was evaluated using agar diffusion test. It was shown that the сhitosan beads modified with nanostructured silver exhibited an antibacterial effect against the tested strains, and they can be used as a basis for creating biodegradable wound healing dressings with a prolonged antibacterial effect. chitosan, silver nanoparticles, antibacterial activity, wound dressings This work was supported by the "Russian Academic Excellence Project 5-100". The study was carried out with the financial support of the Russian Foundation for Basic Research in the framework of the Scientific Project no. 18-29-18039.


2020 ◽  
Vol 21 (10) ◽  
pp. 980-989
Author(s):  
Sampath Shobana ◽  
Sunderam Veena ◽  
S.S.M. Sameer ◽  
K. Swarnalakshmi ◽  
L.A. Vishal

Aims: To evaluate the antibacterial activity of Artocarpus hirsutus mediated seed extract for nanoparticle synthesis. Background: Gastrointestinal bacteria are known for causing deadly infections in humans. They also possess multi-drug resistance and interfere with clinical treatments. Applied nanotechnology has been known to combat such infectious agents with little interference from their special attributes. Here we synthesize silver nanoparticles from Artocarpus hirsutus seed extract against two gastro-intestinal bacterial species: Enterobacter aerogenes and Listeria monocytogenes. Objective: To collect, dry, and process seeds of Artocarpus hirsutus for nanoparticle synthesis. To evaluate the morphological interaction of silver nanoparticles with bacteria. Methods: Artocarpus hirsutus seeds were collected and processed and further silver nanoparticles were synthesized by the co-precipitation method. The synthesized nanoparticles were characterized using XRD, UV, FTIR, and SEM. These nanoparticles were employed to study the antibacterial activity of nanoparticles against Enterobacter aerogenes and Listeria monocytogenes using well diffusion method. Further, morphological interaction of silver nanoparticles on bacteria was studied using SEM. Result: Silver nanoparticles were synthesized using Artocarpus hirsutus seed extract and characterization studies confirmed that silver nanoparticles were spherical in shape with 25-40 nm size. Antibacterial study exhibited better activity against Enterobacter aerogenes with a maximum zone of inhibition than on Listeria monocytogenes. SEM micrographs indicated that Enterobacter aerogenes bacteria were more susceptible to silver nanoparticles due to the absence of cell wall. Also, the size and charge of silver nanoparticles enable easy penetration of the bacterial cell wall. Conclusion: In this study, silver nanoparticles were synthesized using the seed extract of Artocarpus hirsutus for the first time exploiting the fact that Moraceae species have high phytonutrient content which aided in nanoparticle synthesis. This nanoparticle can be employed for large scale synthesis which when coupled with the pharmaceutical industry can be used to overcome the problems associated with conventional antibiotics to treat gastrointestinal bacteria.


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