THE ROLE OF DETERMINING THE LEVELS OF SERUM COLLAGEN TYPE IV IN DIAGNOSING EARLY DIABETIC NEPHROPATHY

In healthy glomeruli, parietal epithelial cell (PEC)-derived extracellular matrix (ECM) proteins include laminin-β1, perlecan, and collagen type IV-α2 and podocyte-specific ECM proteins include laminin-β2, agrin, and collagen type IV-α4. This study aimed to define individual ECM protein isoform expression by PECs in both experimental and human focal segmental glomerulosclerosis (FSGS) and diabetic nephropathy (DN) and to determine if changes were CD44 dependent. In experimental FSGS induced with a cytotoxic podocyte antibody and in the BTBR ob/ob mouse model of DN, PEC-derived protein staining was significantly increased in PECs. Dual staining also showed de novo expression of the podocyte-specific ECM proteins laminin-β2 and agrin in PECs. Similar findings were observed in biopsies from patients with FSGS and DN. Increases in individual ECM proteins colocalized with CD44 in PECs in disease. To determine the role of CD44, FSGS was induced in CD44−/− and CD44+/+ mice. PEC staining for perlecan, collagen type IV-α2, laminin-β2, and agrin were significantly lower in diseased CD44−/− mice compared with diseased CD44+/+ mice. These results show that in experimental and human FSGS and DN, PECs typically in an activated state, produce both PEC-derived and podocyte-specific ECM protein isoforms, and that the majority of these changes were dependent on CD44.

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Serum anti-collagen type IV IgM antibodies and development of diabetic nephropathy in diabetics with essential hypertension

Central European Journal of Immunology ◽

10.5114/ceji.2015.56966 ◽

2016 ◽

Vol 1 ◽

pp. 86-92 ◽

Cited By ~ 4

Author(s):

Asparuh Nikolov ◽

Ivan Tsinlikov ◽

Ivanka Tsinlikova ◽

George Nicoloff ◽

Alexander Blazhev ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Essential Hypertension ◽

Collagen Type ◽

Collagen Type Iv ◽

Igm Antibodies ◽

Type Iv

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Role of myofibroblasts and collagen type IV in patients of IgA nephropathy as markers of renal dysfunction

Indian Journal of Nephrology ◽

10.4103/0971-4065.62098 ◽

2010 ◽

Vol 20 (1) ◽

pp. 34 ◽

Cited By ~ 6

Author(s):

RW Minz ◽

A Bakshi ◽

S Chhabra ◽

K Joshi ◽

V Sakhuja

Keyword(s):

Renal Dysfunction ◽

Iga Nephropathy ◽

Collagen Type ◽

Collagen Type Iv ◽

Type Iv

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The role of microtubules in growth cone turning at substrate boundaries.

The Journal of Cell Biology ◽

10.1083/jcb.128.1.127 ◽

1995 ◽

Vol 128 (1) ◽

pp. 127-137 ◽

Cited By ~ 84

Author(s):

E Tanaka ◽

M W Kirschner

Keyword(s):

Growth Cone ◽

Collagen Type ◽

Axon Growth ◽

Growth Cones ◽

Collagen Type Iv ◽

Early Step ◽

Growth Cone Collapse ◽

Type Iv ◽

And Behaviors

To understand the role of microtubules in growth cone turning, we observed fluorescently labeled microtubules in neurons as they encountered a substrate boundary. Neurons growing on a laminin-rich substrate avoided growing onto collagen type IV. Turning growth cones assumed heterogeneous morphologies and behaviors that depended primarily in their extent of adhesion to the substrate. We grouped these behaviors into three categories-sidestepping, motility, and growth-mediated reorientation. In sidestepping and motility-mediated reorientation, the growth cone and parts of the axon were not well attached to the substrate so the acquisition of an adherent lamella caused the entire growth cone to move away from the border and consequently reoriented the axon. In these cases, since the motility of the growth cone dominates its reorientation, the microtubules were passive, and reorientation occurred without significant axon growth. In growth-mediated reorientation, the growth cone and axon were attached to the substrate. In this case, microtubules reoriented within the growth cone to stabilize a lamella. Bundling of the reoriented microtubules was followed by growth cone collapse to form new axon, and further, polarized lamellipodial extension. These observations indicate that when the growth cone remains adherent to the substrate during turning, the reorientation and bundling of microtubules is an important, early step in growth cone turning.

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Serum Collagen Type IV for the Assessment of Fibrosis and Resistance to Interferon Therapy in Chronic Hepatitis C

Scandinavian Journal of Gastroenterology ◽

10.3109/00365529409096841 ◽

1994 ◽

Vol 29 (5) ◽

pp. 474-479 ◽

Cited By ~ 23

Author(s):

K. Yabu ◽

K. Kiyosawa ◽

H. Mori ◽

A. Matsumoto ◽

K. Yoshizawa ◽

...

Keyword(s):

Chronic Hepatitis ◽

Hepatitis C ◽

Chronic Hepatitis C ◽

Interferon Therapy ◽

Collagen Type ◽

Collagen Type Iv ◽

Type Iv ◽

Serum Collagen

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Camel milk attenuates the biochemical and morphological features of diabetic nephropathy: Inhibition of Smad1 and collagen type IV synthesis

Chemico-Biological Interactions ◽

10.1016/j.cbi.2015.01.013 ◽

2015 ◽

Vol 229 ◽

pp. 100-108 ◽

Cited By ~ 21

Author(s):

Aida A. Korish ◽

Abdel Galil Abdel Gader ◽

Hesham M. Korashy ◽

Abdul Majeed Al-Drees ◽

Abdulqader A. Alhaider ◽

...

Keyword(s):

Diabetic Nephropathy ◽

Collagen Type ◽

Morphological Features ◽

Camel Milk ◽

Collagen Type Iv ◽

Type Iv

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Role of collagen type IV in the pathogenesis of increased prenasal thickness in Down syndrome fetuses: sonographic and immunohistological findings

Journal of Perinatal Medicine ◽

10.1515/jpm-2015-0419 ◽

2017 ◽

Vol 45 (2) ◽

Cited By ~ 2

Author(s):

Ron Maymon ◽

Sonia Mendlovic ◽

Yaakov Melcer ◽

Tal Sarig-Meth ◽

Lilian Habler ◽

...

Keyword(s):

Down Syndrome ◽

Blood Vessels ◽

Type Iv Collagen ◽

Collagen Type ◽

Control Group ◽

Collagen Type Iv ◽

Type Iv ◽

Products Of Conception ◽

Tissue Specimens

AbstractObjective:The present study aims to compare the presence and localization of collagen type IV in the prenasal tissue of fetuses with and without Down syndrome (DS).Methods:Products of conception were obtained from mid-gestation uterine evacuations of 14 DS fetuses and 15 unaffected controls. Microdissection of the prenasal area and an analysis of the prenasal tissue specimens were performed by a single pathologist, blinded to the karyotype results. Immunohistological presence and localization of type IV collagen were analyzed in the basement membrane (BM), blood vessels, and stroma of the tissues.Results:There were no statistically significant differences in the presence and localization of antibodies for collagen IV in the blood vessels and stroma between the two groups. However, the presence and localization of type IV collagen in the BM of the prenasal skin were significantly higher in DS specimens compared to the control group (P=0.023). When combining both groups altogether, a significant correlation was found between the increased prenasal thickness (PT) and the high presence and location of collagen type IV, irrespective of the karyotype results (Spearman’s correlation; R=0.459; P=0.012).Conclusion:Using the immunohistochemistry technique, we were able to confirm the overexpression of collagen type IV in the BM of the prenasal area. This may explain the sonographic finding of increased PT seen mainly in DS fetuses.

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