scholarly journals Expression and function of a putative cell surface receptor for fibronectin in hamster and human cell lines.

1986 ◽  
Vol 103 (4) ◽  
pp. 1595-1603 ◽  
Author(s):  
P J Brown ◽  
R L Juliano
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Cell Lines ◽  
Human Cell ◽  
Mammalian Cells ◽  
Surface Glycoprotein ◽  
Cell Surface Receptor ◽  
Human Cell Lines ◽  
Cell Surface Glycoprotein ◽  
Fibronectin Receptor

We have previously reported the use of monoclonal antibodies to identify a 140-kD cell surface glycoprotein in mammalian cells that is specifically involved in fibronectin-mediated cell adhesion. We now report the purification of this molecule using immunoaffinity chromatography and the subsequent generation of polyclonal antibodies that selectively immunoprecipitate 140-kD putative fibronectin receptor glycoprotein (gp140) extracted from rodent or human cells; these antibodies also specifically block fibronectin-mediated cell adhesion but not adhesion mediated by other factors in serum. Expression of gp140-like molecules was detected on the surfaces of several adherent human cell lines (HDF, WISH, and EFC) but not on erythrocytes; however, gp140 was also detected on a nonadherent human lymphoid line (DAUDI). Analysis of gp140 on nonreducing SDS gels revealed two closely migrating bands. Protease digestion and peptide mapping suggests that the two bands are closely related polypeptides.

Human cell-surface glycoprotein with unusual properties

Nature ◽  
1980 ◽  
Vol 286 (5776) ◽  
pp. 888-891 ◽  
Author(s):  
M. Bishr Omary ◽  
Ian S. Trowbridge ◽  
Jun Minowada
Keyword(s):  
Cell Surface ◽  
Human Cell ◽  
Surface Glycoprotein ◽  
Cell Surface Glycoprotein

Evidence for Cell-Surface Association Between Fusin and the CD4-gp120 Complex in Human Cell Lines

Science ◽  
1996 ◽  
Vol 274 (5287) ◽  
pp. 602-605 ◽  
Author(s):  
C. K. Lapham ◽  
J. Ouyang ◽  
B. Chandrasekhar ◽  
N. Y. Nguyen ◽  
D. S. Dimitrov ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cell Lines

scholarly journals Extracellular K+ concentration controls cell surface density of IKr in rabbit hearts and of the HERG channel in human cell lines

2009 ◽  
Vol 119 (9) ◽  
pp. 2745-2757 ◽  
Author(s):  
Jun Guo ◽  
Hamid Massaeli ◽  
Jianmin Xu ◽  
Zongchao Jia ◽  
Jeffrey T. Wigle ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Human Cell ◽  
Surface Density ◽  
Herg Channel ◽  
Human Cell Lines ◽  
K Concentration

scholarly journals HBO1 (KAT7) Does Not Have an Essential Role in Cell Proliferation, DNA Replication, or Histone 4 Acetylation in Human Cells

2019 ◽  
Vol 40 (4) ◽  
Author(s):  
Andrew J. Kueh ◽  
Samantha Eccles ◽  
Leonie Tang ◽  
Alexandra L. Garnham ◽  
Rose E. May ◽  
...  
Keyword(s):  
Cell Proliferation ◽  
Cell Adhesion ◽  
Dna Replication ◽  
Cell Lines ◽  
Human Cell ◽  
Human Cells ◽  
Human Cell Lines ◽  
Hela Cell Lines ◽  
Mouse Tissues ◽  
Sirna Knockdown

ABSTRACT HBO1 (MYST2/KAT7) is essential for histone 3 lysine 14 acetylation (H3K14ac) but is dispensable for H4 acetylation and DNA replication in mouse tissues. In contrast, previous studies using small interfering RNA (siRNA) knockdown in human cell lines have suggested that HBO1 is essential for DNA replication. To determine if HBO1 has distinctly different roles in immortalized human cell lines and normal mouse cells, we performed siRNA knockdown of HBO1. In addition, we used CRISPR/Cas9 to generate 293T, MCF7, and HeLa cell lines lacking HBO1. Using both techniques, we show that HBO1 is essential for all H3K14ac in human cells and is unlikely to have a direct effect on H4 acetylation and only has minor effects on cell proliferation. Surprisingly, the loss of HBO1 and H3K14ac in HeLa cells led to the secondary loss of almost all H4 acetylation after 4 weeks. Thus, HBO1 is dispensable for DNA replication and cell proliferation in immortalized human cells. However, while cell proliferation proceeded without HBO1 and H3K14ac, HBO1 gene deletion led to profound changes in cell adhesion, particularly in 293T cells. Consistent with this phenotype, the loss of HBO1 in both 293T and HeLa principally affected genes mediating cell adhesion, with comparatively minor effects on other cellular processes.

scholarly journals Expression analysis of secreted and cell surface genes of five transformed human cell lines and derivative xenograft tumors

BMC Genomics ◽  
2005 ◽  
Vol 6 (1) ◽  
Author(s):  
Robert A Stull ◽  
Roya Tavassoli ◽  
Scot Kennedy ◽  
Steve Osborn ◽  
Rachel Harte ◽  
...  
Keyword(s):  
Cell Surface ◽  
Cell Lines ◽  
Expression Analysis ◽  
Human Cell ◽  
Human Cell Lines
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