scholarly journals ELECTRON MICROSCOPY OF CARTILAGE AND BONE MATRIX AT THE DISTAL EPIPHYSEAL LINE OF THE FEMUR IN THE NEWBORN INFANT

1956 ◽  
Vol 2 (4) ◽  
pp. 253-260 ◽  
Author(s):  
Robert A. Robinson ◽  
D. A. Cameron
Keyword(s):  
Electron Microscopy ◽  
Newborn Infant ◽  
Bone Matrix ◽  
Central Zone ◽  
Epiphyseal Cartilage ◽  
Extracellular Matrices ◽  
Cartilage Calcification ◽  
The Third ◽  
Calcified Cartilage ◽  
And Cartilage

An examination of the fine structure of cartilage and bone matrix at the distal epiphyseal line of the femur of a newborn infant has revealed the following information. Cartilage matrix is composed of a network of widely spaced fibers without obvious periodic banding. Calcification is first seen about the level of the third chondrocyte capsule distal to the furthest penetration of the capillaries. It starts as a haphazard deposition of crystals which have no obvious relationship to the location of the fibers. The process of calcification is completed before ossification commences but the central zone of matrix remains only partly mineralized. Bone matrix is formed over a bar of calcified cartilage. Fibers, recognizable as collagen, are deposited in a loose network in a narrow zone between the osteoblasts and cartilage. These fibers are 2 to 5 times as wide as the fibers in epiphyseal cartilage. Calcification then begins in the osteoid, crystals being first laid down irregularly on or close to the fibers. As they increase in number, the crystals tend to line up along the fibers and eventually are arranged so that the periodicity of the underlying collagen is emphasized. In such an area the fibers are more tightly packed than when uncalcified. There is no change observed in the calcified cartilage at this level. The extracellular matrices of this epiphyseal cartilage and bone can be distinguished from one another in the electron microscope.

Studies on Thrombolysis with Streptokinase

1971 ◽  
Vol 25 (02) ◽  
pp. 354-378 ◽  
Author(s):  
R Gottlob ◽  
L Stockinger ◽  
U Pötting ◽  
G Schattenmann
Keyword(s):  
Electron Microscopy ◽  
Cross Section ◽  
Whole Blood ◽  
Jugular Vein ◽  
Thin Sections ◽  
The Third ◽  
Morphological Findings ◽  
Blood Clots ◽  
Arteries And Veins

SummaryIn vitro whole blood clots of various ages, experimental thrombi produced in the jugular vein of rabbits and human thrombi from arteries and veins were examined in semi-thin sections and by means of electron microscopy.In all types of clots examined a typical course of retraction was found. Retraction starts with a dense excentrical focus which grows into a densification ring. After 24 hours the entire clot becomes almost homogeneously dense; later a secondary swelling sets in.Shortly after coagulation the erythrocytes on the rim of the clot are bi-concave discs. They then assume the shape of crenate spheres, turn into smooth spheres and finally become indented ghosts which have lost the largest part of their contents. In the inner zone, which makes up the bulk of the clot, we observed bi-concave discs prior to retraction. After retraction we see no crenations but irregularly shaped erythrocytes. Once the secondary swelling sets in, the cross-section becomes polygonal and later spherical. After extensive hemolysis we observe the “retiform thrombus” made up of ghosts.Experimental and clinical thrombi present the same morphology but are differentiated from in vitro clots by: earlier hemolysis, immigration of leukocytes, formation of a rim layer consisting of fibrin and thrombocytes, and the symptoms of organization. Such symptoms of organization which definitely will prevent lysis with streptokinase were found relatively late in experimental and clinical thrombi. Capillary buds and capillary loops were never found in clinical thrombi prior to the third month.The morphological findings agree with earlier physical and enzymatic investigations. The observation that phenomena of reorganization occur relatively late and frequently only in the rim areas of large thrombi explains why lytic therapy is possible in some of the chronic obliterations.

An emendation of the generic diagnosis of Phoreiobothrium Linton, 1889 (Tetraphyllidea: Onchobothriidae) with a detailed description of bothridia and hooks

1985 ◽  
Vol 63 (5) ◽  
pp. 1199-1206 ◽  
Author(s):  
J. N. Caira
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Monophyletic Group ◽  
Generic Diagnosis ◽  
The Third ◽  
Scanning Electron

Hook terminology for the three-pronged hooks of Phoreiobothrium Linton, 1889 is reconciled with that for two-pronged hooks such that the two outermost prongs are considered homologous with the prongs of a two-pronged hook and the third inner prong is termed the basal prong. Scanning electron microscopy was performed on the scolex of Phoreiobothrium lasium Linton, 1889 and the solid nature of the bothridia was reconfirmed. Examination of material of all four species of Phoreiobothrium leads to the conclusion that each species possesses three-pronged hooks that are hollow and open to the outside via pores. The bothridia of each species are considered to be horizontally divided into two loculi, the posterior one being recessed and vertically subdivided. The diagnosis of the genus Phoreiobothrium is emended, and the four species allocated to it are redescribed. Phoreiobothrium is determined to be a monophyletic group on the basis of two synapomorphies and a key to the four species of the genus is presented.

The Mechanism of active capture of animal food by the Sergestid Shrimp Acetes Sibogae Australis

1998 ◽  
Vol 78 (2) ◽  
pp. 497-508 ◽  
Author(s):  
Lachlan Mcleay ◽  
C.G. Alexander
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Prey Capture ◽  
Size Range ◽  
The Third ◽  
Feeding Modes ◽  
In Captivity ◽  
Combined Actions ◽  
Wide Size Range ◽  
Scanning Electron

Combining the use of scanning electron microscopy and microcinematography with functional and behavioural observations has clarified many aspects underlying the feeding processes of the small planktonic sergestid shrimp Acetes sibogae australis. In captivity Acetes sibogae australis is an opportunistic feeder that uses four principal feeding modes to capture a wide size range of prey: Artemia nauplii (<0.33 mm), copepods (<1mm) and moribund Acetes (up to 25 mm). Prey capture is effected by combined actions of the first three pairs of pereiopods and the third maxillipeds before transfer to the more dorsal second maxillipeds. The second maxillipeds are the principal appendages used in securing, manipulating, sorting and rejecting prey before insertion into the vicinity of the inner mouthparts.

Mucolipidosis Type III α/β: The First Characterization of This Rare Disease by Autopsy

2011 ◽  
Vol 135 (4) ◽  
pp. 503-510
Author(s):  
Darcy A Kerr ◽  
Vincent A Memoli ◽  
Sara S Cathey ◽  
Brent T Harris
Keyword(s):  
Electron Microscopy ◽  
Heart Valves ◽  
Light And Electron Microscopy ◽  
Lysosomal Storage ◽  
Cytoplasmic Granules ◽  
Type Iii ◽  
Mucolipidosis Type ◽  
Electron Lucent ◽  
And Cartilage

Abstract We report findings from an autopsy of a 45-year-old woman with the rare lysosomal storage disease mucolipidosis type III α/β. Her disease manifested most notably as multiple bone and cartilage problems with tracheal and bronchial malacia. Principal autopsy findings included gross abnormalities in bone and cartilage with corresponding microscopic cytoplasmic lysosomal granules. These cytoplasmic granules were also seen in histologic preparations of the brain, myocardium, heart valves, and fibroblasts of the liver and skin by light and electron microscopy. By electron microscopy there were scattered, diffuse vesicular cytoplasmic granules in neurons and glia and an increase in lysosomal structures with fine electron lucent granularity in the above tissue types. Our findings help elaborate current understanding of this disease and differentiate it from the mucopolysaccharidoses and related disorders. To our knowledge, this is the first report to document pathologic findings in a patient with mucolipidosis type III α/β by autopsy.

Identification of larvae of two Aphodius Helwig, 1798 (Coleoptera: Scarabaeidae: Aphodiinae) species using morphology and DNA barcode

Zootaxa ◽  
2021 ◽  
Vol 5047 (2) ◽  
pp. 153-164
Author(s):  
ANDREY V. FROLOV ◽  
MARIA S. VISHNEVSKAYA ◽  
LILIA A. AKHMETOVA
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Dna Barcode ◽  
The Third ◽  
Coi Sequences ◽  
First Time ◽  
Scanning Electron ◽  
Third Instar Larvae

The third instar larvae of Aphodius (Alocoderus) hydrochaeris (Fabricius, 1798) and A. (Bodilus) ictericus (Laicharting, 1781) are described based on scanning electron microscopy and COI sequences. COI barcode sequence for A. (A.) hydrohaeris is provided for the first time. Two haplotypes are discovered in A. (B.) ictericus.  

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