scholarly journals STUDIES ON THE HUMAN CORPUS LUTEUM

1969 ◽  
Vol 41 (3) ◽  
pp. 696-715 ◽  
Author(s):  
Eleanor C. Adams ◽  
Arthur T. Hertig
Keyword(s):  
Corpus Luteum ◽  
Lipid Droplets ◽  
Stellate Cells ◽  
Perivascular Space ◽  
Luteal Cell ◽  
Surface Activities ◽  
Thecal Cells ◽  
A Cell ◽  
Relationship Of ◽  
Human Corpus Luteum

The ultrastructure of huma corpora luntea obtained approximately 2, 3, 5, 11, and 15 days after ovulation is reported. All specimens were fixed in Karnovsky's formaldehyde-glutaral-dehyde solution. The 5-day corpus luteum is presumed to represent, in terms of fine structure, the ultrastructural aspects of high progesterone production and is compared to younger differentiating and older regressing specimens. A distinct topographic relationship of cytoplasmic organelles is noted in the mature 5-day luteal cell. It consists of a peripheral cupshaped mass of tubular ER (endoplasmic reticulum), associated with granular ER, lipid droplets, and mitochondria, which merges with a large paranuclear Golgi area extending to a cell surface bordering a perivascular space. The plasma membrane of active luteal cells is described and its variations suggest areas of specialized surface activities. The prevalence and ultrastructure of more electron-opaque stellate cells, of phagocytes, and of thecal cells are reported.

scholarly journals Differential distribution of gelatinases and tissue inhibitor of metalloproteinase-1 in the rat ovary

1998 ◽  
Vol 158 (2) ◽  
pp. 221-228 ◽  
Author(s):  
P Bagavandoss
Keyword(s):  
Plasma Membrane ◽  
Corpus Luteum ◽  
Tissue Inhibitor Of Metalloproteinase ◽  
Neonatal Rat ◽  
Luteal Cell ◽  
Surface Epithelium ◽  
Tissue Inhibitor ◽  
Differential Distribution ◽  
Thecal Cells ◽  
Rat Ovary

The distribution of gelatinases/matrix metalloproteinases-2 and -9 (MMP-2 and MMP-9) and tissue inhibitor of metalloproteinase-1 (TIMP-1) in neonatal and gonadotropin-primed immature rat ovaries was studied by immunofluorescent microscopy. Immature female Long-Evans rats were primed with 15 IU pregnant mare's serum gonadotropin (PMSG) in 100 microliters PBS. Two days later, to induce ovulation, the rats were injected with human chorionic gonadotropin (hCG, 5 IU/100 microliters PBS). The animals were killed at appropriate times and the ovaries removed and processed for cryostat or paraffin sectioning. Ovaries were also obtained from 7-day-old neonatal rats and processed as above. In the neonatal rat ovary, MMP-2 was present in the follicle and in the ovarian surface epithelium. MMP-9 was not detectable in the neonatal ovary. TIMP-1 was present in the oocyte and in the surface epithelium. In the PMSG-primed ovary, MMP-2 was present in the granulosa and thecal cells of the ovary. MMP-9 distribution, however, was restricted to the interstitial and thecal cells. TIMP-1 was mainly present in the blood vessels and thecal cells, with minor staining in the granulosa cells. In the developing corpus luteum, luteal and endothelial cells were positive for MMP-2. MMP-9 localization was restricted to the plasma membrane of the luteal and interstitial cells. TIMP-1 was clearly observed in the luteal capillaries and, to a lesser extent, in the luteal cell plasma membrane. This distribution of MMP-2, MMP-9, and TIMP-1 in the corpus luteum persisted throughout the life span of the corpus luteum. The spatial and temporal distribution of the gelatinases and TIMP-1 suggests unique roles for these proteins in the rat ovary.

Interrelation between luteal cell types in steroidogenesis in vitro of human corpus luteum

1983 ◽  
Vol 19 (1) ◽  
pp. 811-815 ◽  
Author(s):  
Mori Takahide ◽  
Nihnobu Kenji ◽  
Takeuchi Satoru ◽  
Onho Yoshio ◽  
Tojo Shimpei
Keyword(s):  
Corpus Luteum ◽  
Cell Types ◽  
Luteal Cell ◽  
Human Corpus Luteum

Varied effects of doxorubicin (DOX) on the corpus luteum of C57BL/6 mice during early pregnancy

2021 ◽  
Author(s):  
Christian Lee Andersen ◽  
Haeyeun Byun ◽  
Yuehuan Li ◽  
Shuo Xiao ◽  
Doris M Miller ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Corpus Luteum ◽  
Early Pregnancy ◽  
Lipid Droplets ◽  
Luteal Cell ◽  
Knowledge Gap ◽  
Chemotherapeutic Drugs ◽  
Luteal Cells ◽  
Single Intraperitoneal Injection ◽  
Premenopausal Patients

Abstract Certain chemotherapeutic drugs are toxic to ovarian follicles. The corpus luteum (CL) is normally developed from an ovulated follicle for producing progesterone (P4) to support early pregnancy. To fill in the knowledge gap about effects of chemotherapy on the CL, we tested the hypothesis that chemotherapy may target endothelial cells and/or luteal cells in the CL to impair CL function in P4 steroidogenesis using doxorubicin (DOX) as a representative chemotherapeutic drug in mice. In both mixed background mice and C57BL/6 mice, a single intraperitoneal injection of DOX (10 mg/kg) on 0.5 days post coitum (D0.5, post-ovulation) led to ~58% D3.5 mice with serum P4 levels lower than the serum P4 range in the PBS-treated control mice. Further studies in the C57BL/6 ovaries revealed that CLs from DOX-treated mice with low P4 levels had less defined luteal cords and disrupted collagen IV expression pattern, indicating disrupted capillary, accompanied with less differentiated luteal cells that had smaller cytoplasm and reduced StAR expression. DOX-treated ovaries had increased granulosa cell death in the growing follicles, reduced PCNA-positive endothelial cells in the CLs, enlarged lipid droplets and disrupted F-actin in the luteal cells. These novel data suggest that the proliferating endothelial cells in the developing CL may be the primary target of DOX to impair the vascular support for luteal cell differentiation and subsequently P4 steroidogenesis. This study fills in the knowledge gap about the toxic effects of chemotherapy on the CL and provides critical information for risk assessment of chemotherapy in premenopausal patients.

Association of progesterone with a unique particulate fraction of the human corpus luteum

1988 ◽  
Vol 116 (2) ◽  
pp. 307-312 ◽  
Author(s):  
T. A. Bramley ◽  
G. S. Menzies
Keyword(s):  
Plasma Membrane ◽  
Corpus Luteum ◽  
Plasma Membranes ◽  
Buoyant Density ◽  
Particulate Fraction ◽  
Luteal Cell ◽  
Cell Plasma Membrane ◽  
Intracellular Organelle ◽  
Cell Plasma ◽  
Human Corpus Luteum

ABSTRACT Homogenates of human corpus luteum were fractionated on continuous sucrose density gradients, with and without pretreatment with digitonin to perturb plasma membranes. Fractions of each gradient were assayed for steroid content and a range of plasma membrane and intracellular organelle markers. Progesterone and oestradiol were associated with a particulate fraction (buoyant density, 1·08–1·13 g/cm3). The buoyant density distribution of these steroids was distinct from those of the luteal cell plasma membrane and intracellular organelle markers tested. Treatment with digitonin increased the buoyant density of both progesterone and oestradiol. If steroids are contained in distinct vesicles, these vesicles may be involved in the sequestration of newly synthesized steroid and its movement to the cell surface for release into the circulation. J. Endocr. (1988) 116, 307–312

scholarly journals Cellular composition of the Corpus luteum of Indian fruit bat, Rousettus leschenaulti (Desmarest) during early embryonic development

2016 ◽  
Vol 3 (2) ◽  
Author(s):  
Badiye V.H
Keyword(s):  
Endoplasmic Reticulum ◽  
Corpus Luteum ◽  
Lipid Droplets ◽  
Blastocyst Stage ◽  
Limb Bud ◽  
Luteal Cell ◽  
Bud Formation ◽  
Luteal Cells ◽  
Fruit Bat ◽  
Rousettus Leschenaulti

The fine structure of luteal cell of the corpus luteum of Indian fruit bat, Rousettus leschenaulti was studied at three stages unilaminar blastocyst stage, Implanted bilaminar blastocyst stage and limb bud stage of early pregnancy. At unilaminar blastocyst stage luteal cells had small nuclei euchromatin. Mitochondria were small, round shaped with tubular cristae. Numerous less osmiophilic lipid droplets were observed in cytoplasmic field of the luteal cells. After implantation at implanted bilaminar blastocyst stage nuclear heterochromatin were reduced and nucleoli were larger and complex. Mitochondria were enlarged and often bizarre shaped with tubular cristae. Golgi complex and agranular endoplasmic reticulum were more conspicuous. Lipid droplets were less osmiophilic. At the stage of limb bud formation the luteal cells suggests different morphological picture, the nuclear size is reduced with clumps of heterochromatin. The agranular endoplasmic reticulum assumes the form of bundles of parallel tubules dispersed in several planes. Mitochondrial size was reduced then the previous stage and they posses vesicular cristae. These observations suggest that the steroidogenic activity of the luteal cells is highest during implantation and comparatively regresses during limb bud formation. It is suggested that the luteal cells is an important ovarian source of pregnancy hormones.

INTERRELATION BETWEEN LUTEAL CELL TYPES IN STEROIDOGENESIS IN VITRO OF HUMAN CORPUS LUTEUM

1983 ◽  
pp. 811-815
Author(s):  
TAKAHIDE MORI ◽  
KENJI NIHNOBU ◽  
SATORU TAKEUCHI ◽  
YOSHIO ONHO ◽  
SHIMPEI TOJO
Keyword(s):  
Corpus Luteum ◽  
Cell Types ◽  
Luteal Cell ◽  
Human Corpus Luteum

scholarly journals STUDIES ON THE HUMAN CORPUS LUTEUM

1969 ◽  
Vol 41 (3) ◽  
pp. 716-735 ◽  
Author(s):  
Eleanor C. Adams ◽  
Arthur T. Hertig
Keyword(s):  
Central Area ◽  
Luteal Cell ◽  
Corpora Lutea ◽  
Cell Cytoplasm ◽  
Nuclear Membranes ◽  
Outer Leaflet ◽  
Perinuclear Cytoplasm ◽  
A Cell ◽  
Human Corpus Luteum ◽  
Lateral Cell

The ultrastructure of human corpora lutea obtained during the 6th, 10th, 16th, and 35th week of pregnancy is reported. Differences between the established luteal cell of pregnancy and the transitory luteal cell of the menstrual cycle are noted. In pregnancy the luteal cell is more compartmentalized into a peripheral mass of ER (endoplasmic reticulum) and a central area where mitochondria and Golgi complexes are concentrated. The latter area extends to a cell surface where microvilli face on a perivascular space. Long bundles of filaments are prominent within the luteal cell cytoplasm and, in contiguous cells, appear to arise from adjacent desmosomal regions. Bilateral subsurface cisternae of granular ER at lateral cell borders appear to be areas of specialized junctional surfaces. Certain luteal cells with irregular nuclear membranes are also characterized by vesicular aggregates enclosed within a single membrane. These aggregates are found within the peripheral nucleoplasm or the perinuclear cytoplasm. Their single limiting membrane often appears continuous with either the inner or outer leaflet of the nuclear membrane.

Pulsatile secretion of progesterone from the human corpus luteum: poor correlation with bioactive LH pulses

1986 ◽  
Vol 111 (4) ◽  
pp. 553-557 ◽  
Author(s):  
Inese Z. Beitins ◽  
Maria L. Dufau
Keyword(s):  
Corpus Luteum ◽  
Temporal Relationship ◽  
Pulse Amplitude ◽  
Pulse Frequency ◽  
Biologically Active ◽  
Poor Correlation ◽  
Serum Progesterone ◽  
Progesterone Secretion ◽  
Wide Range ◽  
Human Corpus Luteum

Abstract. Having previously established that biologically active luteinizing hormone (LH) is secreted in episodic pulsations that vary in relation to the menstrual cycle, we investigated the possibility that a temporal relationship could exist between the bioactive LH pulses and progesterone secretion from the late corpus luteum. In 4 young women blood was withdrawn every 15 min for 8 h. Serum progesterone concentrations fluctuated at a mean frequency of 0.9 h with a wide range of amplitudes (13.8 to 1.7 ng/ml). Serum bioactive LH pulse frequency in contrast was 0.25 pulses/h in all subjects. The pulse amplitude was 18.2 to 12.4 mIU/ml (2nd IRP-hMG). These data reveal that within the 8 h-period studied, progesterone secretory pulses occurred four times more frequently as those for bioactive LH. Therefore it is unlikely that a temporal relationship exists between individual bioactive LH and pulses of progesterone secreted by the late corpus luteum.

Sign in / Sign up

Export Citation Format

Share Document