scholarly journals A ZONAL ROTOR METHOD FOR THE PREPARATION OF MICROPEROXISOMES FROM EPITHELIAL CELLS OF GUINEA PIG SMALL INTESTINE

1974 ◽  
Vol 61 (1) ◽  
pp. 123-133 ◽  
Author(s):  
M. J. Connock ◽  
P. R. Kirk ◽  
A. P. Sturdee
Keyword(s):  
Small Intestine ◽  
Electron Microscope ◽  
Epithelial Cells ◽  
Guinea Pig ◽  
Analytical Data ◽  
Microscope Examination ◽  
Electron Microscope Examination ◽  
Large Particles ◽  
Zonal Separation ◽  
Zonal Rotor

A method is described for the preparation of catalase particles from homogenates made from suspensions of epithelial cells of the small intestine of the guinea pig. Electron microscope examination of the preparations revealed the presence of small diaminobenzidine-positive particles measuring 0.1–0.3 nm in diameter and resembling the microperoxisomes observed by Novikoff and Novikoff (1972. J. Cell Biol. 53:532.). Analytical data upon which the method is based are presented. The technique consisted of a rate zonal separation of microperoxisomes from large particles followed by an isopycnic separation from less dense organelles. Application of the method yielded microperoxisomes purified between 20- and 30-fold.

In vitro development of isolated ectoderm from axolotl gastrulae

Development ◽  
1984 ◽  
Vol 80 (1) ◽  
pp. 321-330
Author(s):  
Jonathan M. W. Slack
Keyword(s):  
Electron Microscopy ◽  
Electron Microscope ◽  
Light Microscopy ◽  
Outer Layer ◽  
Microscope Examination ◽  
Animal Pole ◽  
In Vitro Development ◽  
Electron Microscope Examination ◽  
Vitro Development

The development of ectoderm isolated from the animal pole of axolotl gastrulae is monitored by light microscopy, electron microscopy and analysis of newly synthesized proteins, glycoproteins and glycolipids. When control embryos are undergoing neurulation it is shown that the explants autonomously begin to express epidermal markers and do not express mesodermal markers. However the results suggest that not all the cells become epidermal and electron microscope examination shows that only the outer layer does so, the inner cells remaining undifferentiated.

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