STABILITY OF DNA IN PURKINJE CELL NUCLEI OF THE MOUSE

Neurons of the mouse were labeled with [3H]thymidine during their prenatal period of proliferation. The 3H activity of the Purkinje cell nuclei was then studied autoradiographically 8, 25, 55, and 90 days after birth. The measured grain number per nucleus decreased by about 14% between the 8th and 25th postnatal days and then remained constant up to 90 days. There was no significant decrease of the 3H activity of the Purkinje cell nuclei after correction of the measured grain number per nucleus for increasing nuclear volume of the growing Purkinje cells and for the influence of [3H]ß self-absorption in the material of the sections. Injection of a high dose of [3H]thymidine into young adult mice did not result in 3H labeling of either Purkinje or other neurons in other brain regions. The results agree with the concept of metabolic stability of nuclear DNA. "Metabolic" DNA could not be observed in these experiments.

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Review for "A Neutralizing Antibody Targeting Oxidized Phospholipids Promotes Bone Anabolism in Chow‐Fed Young Adult Mice"

10.1002/jbmr.4173/v1/review2 ◽

2020 ◽

Author(s):

Jung-Min Koh

Keyword(s):

Young Adult ◽

Neutralizing Antibody ◽

Oxidized Phospholipids ◽

Adult Mice ◽

Antibody Targeting

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Review for "A Neutralizing Antibody Targeting Oxidized Phospholipids Promotes Bone Anabolism in Chow‐Fed Young Adult Mice"

10.1002/jbmr.4173/v1/review1 ◽

2020 ◽

Keyword(s):

Young Adult ◽

Neutralizing Antibody ◽

Oxidized Phospholipids ◽

Adult Mice ◽

Antibody Targeting

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Faculty Opinions recommendation of Axon regeneration in young adult mice lacking Nogo-A/B.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1012951.191051 ◽

2003 ◽

Author(s):

Mark Tuszynski

Keyword(s):

Young Adult ◽

Axon Regeneration ◽

Adult Mice

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Immunogenicity and Neutralizing Activity Comparison of SARS-CoV-2 Spike Full-Length and Subunit Domain Proteins in Young Adult and Old-Aged Mice

Vaccines ◽

10.3390/vaccines9040316 ◽

2021 ◽

Vol 9 (4) ◽

pp. 316

Author(s):

Ki-Hye Kim ◽

Noopur Bhatnagar ◽

Subbiah Jeeva ◽

Judy Oh ◽

Bo Ryoung Park ◽

...

Keyword(s):

Young Adult ◽

Receptor Binding ◽

Vaccine Dose ◽

The Elderly ◽

Full Length ◽

High Dose ◽

Igg Antibodies ◽

Neutralizing Activity ◽

Aged Mice ◽

Adjuvant Effects

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) continues to be expanding the pandemic disease across the globe. Although SARS-CoV-2 vaccines were rapidly developed and approved for emergency use of vaccination in humans, supply and production difficulties are slowing down the global vaccination program. The efficacy of many different versions of vaccine candidates and adjuvant effects remain unknown, particularly in the elderly. In this study, we compared the immunogenic properties of SARS-CoV-2 full-length spike (S) ectodomain in young adult and aged mice, S1 with receptor binding domain, and S2 with fusion domain. Full-length S was more immunogenic and effective in inducing IgG antibodies after low dose vaccination, compared to the S1 subunit. Old-aged mice induced SARS-CoV-2 spike-specific IgG antibodies with neutralizing activity after high dose S vaccination. With an increased vaccine dose, S1 was highly effective in inducing neutralizing and receptor-binding inhibiting antibodies, although both S1 and S2 subunit domain vaccines were similarly immunogenic. Adjuvant effects were significant for effective induction of IgG1 and IgG2a isotypes, neutralizing and receptor-binding inhibiting antibodies, and antibody-secreting B cell and interferon-γ secreting T cell immune responses. Results of this study provide information in designing SARS-CoV-2 spike vaccine antigens and effective vaccination in the elderly.

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Variations in the expression of θ-C3H alloantigen on thymic and peripheral lymphocytes of newborn and young adult mice

Cellular Immunology ◽

10.1016/0008-8749(74)90240-8 ◽

1974 ◽

Vol 13 (2) ◽

pp. 216-229 ◽

Cited By ~ 6

Author(s):

Arjun D. Chanana ◽

Juerg Schaedeli ◽

Max W. Hess ◽

Hans Cottier

Keyword(s):

Young Adult ◽

Peripheral Lymphocytes ◽

Adult Mice

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Involvement of deoxyribonucleic acid polymerase β in nuclear deoxyribonucleic acid synthesis

Biochemical Journal ◽

10.1042/bj1730309 ◽

1978 ◽

Vol 173 (1) ◽

pp. 309-314 ◽

Cited By ~ 12

Author(s):

T R Butt ◽

W M Wood ◽

E L McKay ◽

R L P Adams

Keyword(s):

Dna Synthesis ◽

Dna Polymerase ◽

Deoxyribonucleic Acid ◽

Nuclear Dna ◽

Dna Polymerase Beta ◽

Cell Nuclei ◽

High Molecular Weight Dna ◽

Dna Polymerase Alpha ◽

Polymerase Beta

The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.

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