scholarly journals Temporal changes of lysosome and phagosome pH during phagolysosome formation in macrophages: studies by fluorescence spectroscopy.

1981 ◽  
Vol 89 (3) ◽  
pp. 645-652 ◽  
Author(s):  
M J Geisow ◽  
P D'Arcy Hart ◽  
M R Young
Keyword(s):  
Fluorescence Spectroscopy ◽  
Cell Population ◽  
External Medium ◽  
Large Cell ◽  
Peritoneal Macrophages ◽  
Ph Change ◽  
Ph Changes ◽  
Indicator Dyes ◽  
Kinetics Of ◽  
Mouse Peritoneal Macrophages

Intravascular pH was measured within the lysosomes and newly formed phagosomes in cultured mouse peritoneal macrophages. The kinetics of pH change in both vacuolar systems was quantitatively determined within a large cell population by fluorescence spectroscopy. Additionally, pH changes within individual phagosomes were followed semiquantitatively using indicator dyes. Two novel findings were made. Firstly, the pH in new phagosomes was transiently driven alkaline (higher than physiological) even when the external medium was buffered at pH 6.5. Secondly, perturbations of phagosome-lysosome fusion had little effect upon phagosomal pH changes, even though the compounds used markedly altered the pH of the lysosomes in resting and phagocytosing cells.

scholarly journals Synthetic neoglycoproteins: a class of regents for detection of sugar-recognizing substances.

1984 ◽  
Vol 32 (10) ◽  
pp. 1091-1098 ◽  
Author(s):  
M Kataoka ◽  
M Tavassoli
Keyword(s):  
Fluorescein Isothiocyanate ◽  
Recognition System ◽  
Peritoneal Macrophages ◽  
Carrier Protein ◽  
Endogenous Lectins ◽  
Developing Area ◽  
Simple Sugar ◽  
Kinetics Of ◽  
Mouse Peritoneal Macrophages ◽  
Specific Sugar

Specific interactions between proteins and sugars have recently been emphasized in many biological systems. To detect sugar-recognizing substances, known as lectin-like substances or endogenous lectins, we describe a method in which various sugars were covalently bound to a carrier protein such as albumin. This neoglycoprotein was stable at -20 degrees C for a period of 6 months. It was conjugated to various cytochemical markers (125I, fluorescein isothiocyanate, colloidal gold, or latex minibead). Detection of the marker then indicates the presence of the sugar-binding protein. Control experiments in the presence of unlabeled neoglycoprotein or specific sugar indicated the specificity of the reaction. This method was used to analyze the kinetics of binding for a mannose-recognition system in the mouse peritoneal macrophages. The data obtained were in agreement with those previously reported. The method can be used for detection of other sugar-recognizing systems as virtually every simple sugar can be bound to a carrier protein to produce these neoglycoproteins. Some of the consideration required for successful production of these reagents are discussed. These synthetic neoglycoproteins are useful in studying the distribution and kinetics of sugar-recognizing systems and may help to further our understanding of this rapidly developing area.

β-Casein monomers as potential flavonoids nanocarriers: Thermodynamics and kinetics of β-casein-naringin binding by fluorescence spectroscopy and surface plasmon resonance

2020 ◽  
Vol 108 ◽  
pp. 104728
Author(s):  
Ana Flávia Coelho Pacheco ◽  
Natália Moreira Nunes ◽  
Hauster Maximiler Campos de Paula ◽  
Yara Luiza Coelho ◽  
Luis Henrique Mendes da Silva ◽  
...  
Keyword(s):  
Surface Plasmon Resonance ◽  
Fluorescence Spectroscopy ◽  
Surface Plasmon ◽  
Plasmon Resonance ◽  
Thermodynamics And Kinetics ◽  
Kinetics Of
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