scholarly journals The interaction in vitro of Pneumocystis carinii with macrophages and L-cells.

1978 ◽  
Vol 147 (1) ◽  
pp. 157-170 ◽  
Author(s):  
H Masur ◽  
T C Jones
Keyword(s):  
Pneumocystis Carinii ◽  
Significant Degree ◽  
Peritoneal Macrophages ◽  
Mononuclear Phagocytes ◽  
Humoral Factors ◽  
L Cells ◽  
Contrast Microscopy ◽  
Typical Morphology ◽  
Mouse Peritoneal Macrophages

A model was developed for studying the interaction between Pneumocystis, rat-derived cells, and humoral factors. Pneumocystis were obtained in large quantity by bronchial lavage of steroid-treated rats. The trophozoite was the predominant form obtained, and it could readily be recognized by phase contrast microscopy. Organisms maintained a typical morphology for at least 3 days in culture, and 10-20% took up radiolabeled nucleotides. Pneumocystis readily adhered to cell surfaces in a similar manner in alveolar macrophages from steroid-treated or normal rats, mouse peritoneal macrophages, and L-cells. Adherent organisms were not interiorized to a significant degree in the absence of antipneumocystis serum. After addition of rabbit antipneumocystis serum, rapid interiorization of organisms occurred from the surface of macrophages but not L-cells. Organisms appeared to be promptly destroyed within macrophages after interiorization. Persisting or multiplying intracellular forms were not seen. Antipneumocystis serum did not morphologically alter Pneumocystis. These observations suggest a role for antibody and mononuclear phagocytes during the immune response to Pneumocystis.

scholarly journals MECHANISMS OF ACQUIRED RESISTANCE IN MOUSE TYPHOID

1966 ◽  
Vol 124 (4) ◽  
pp. 585-600 ◽  
Author(s):  
R. V. Blanden ◽  
G. B. Mackaness ◽  
F. M. Collins
Keyword(s):  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Normal Mouse ◽  
Acquired Resistance ◽  
Peritoneal Macrophages ◽  
Immune Serum ◽  
Humoral Factors ◽  
Intraperitoneal Route ◽  
Mouse Peritoneal Macrophages

Experiments in vitro comparing normal mouse peritoneal macrophages with cells from Salmonella typhimurium-infected mice have shown that the "immune" macrophages have conspicuously enhanced microbicidal properties. Whereas normal macrophages could inactivate only 50 to 60% of intracellular S. typhimurium pretreated with immune serum, cells from infected animals killed virtually all ingested organisms and did so at an accelerated rate. Macrophages from Listeria monocytogenes-infected mice were shown to possess similarly enhanced microbicidal activity against S. typhimurium. Furthermore, the growth of S. typhimurium in the liver and spleen was more effectively restricted in Listeria-infected mice than in animals vaccinated with heat-killed S. typhimurium, even though the Listeria-infected animals possessed no demonstrable cross-reacting antibody to S. typhimurium. The lack of resistance in the mice vaccinated with heat-killed organisms could not be attributed to any deficiency of humoral factors, since the serum from these animals was as effective at promoting phagocytosis and killing by macrophages as serum from actively infected (and demonstrably resistant) mice. Conversely, Salmonella-infected mice were totally resistant to intravenous challenge with L. monocytogenes. The level of resistance in individual animals was related to the numbers of residual Salmonellae remaining in the tissues; mice with heavier residual infections being the more resistant. Specific antiserum from mice vaccinated with heat-killed S. typhimurium was found to be significantly protective only when the intraperitoneal route of challenge was employed. The foregoing studies have been interpreted to mean that enhancement of the microbicidal ability of macrophages is the mechanism of major importance in acquired resistance to S. typhimurium infection in mice.

scholarly journals Prostaglandin E production by human blood monocytes and mouse peritoneal macrophages.

1978 ◽  
Vol 147 (3) ◽  
pp. 952-957 ◽  
Author(s):  
J I Kurland ◽  
R Bockman
Keyword(s):  
Human Peripheral Blood ◽  
Human Lymphocytes ◽  
B Cell Lymphoma ◽  
Peritoneal Macrophages ◽  
Mononuclear Phagocytes ◽  
Prostaglandin E ◽  
Blood Monocytes ◽  
Tissue Macrophage ◽  
Mouse Peritoneal Macrophages

Purified populations of both human peripheral blood monocytes and murine peritoneal macrophages synthesize and release Prostaglandin E in vitro. In contrast, prostaglandin E was detected in neither the supernate fluids from cultures of highly enriched human lymphocytes and granulocytes, nor in nonadherent murine peritoneal cells. Macrophage prostaglandin E production was markedly enhanced by endotoxin, and completely suppressed by indomethacin. All neoplastic monocyte-macrophage cell lines examined elaborated prostaglandin E in vitro, either constitutively or after induction with endotoxin. In contrast, prostaglandin E production could not be detected from either a T- or B-cell lymphoma, whether or not they were treated with endotoxin. These findings thus indicate that the blood monocyte and tissue macrophage represent an important source of prostaglandin E, a function shared by both normal and neoplastic mononuclear phagocytes.

Trypanosoma (Schizotrypanum) dionisii of Pipistrellus pipistrellus (Chiroptera): intra- and extracellular development in vitro

Parasitology ◽  
1972 ◽  
Vol 65 (2) ◽  
pp. 251-263 ◽  
Author(s):  
J. R. Baker ◽  
Sheila M. Green ◽  
Lisbeth A. Chaloner ◽  
Maria Gaborak
Keyword(s):  
Cell Cultures ◽  
Hela Cells ◽  
Peritoneal Macrophages ◽  
Infection Rates ◽  
L Cells ◽  
Pipistrellus Pipistrellus ◽  
Multiple Fission ◽  
Development In Vitro ◽  
Mouse Peritoneal Macrophages

A trypanosome identified as Trypanosoma (Schizotrypanum) dionisii Bettencourt & França, 1905, has been isolated from Pipistrellus pipistrellus (Chiroptera) in England. At least five out of eight P. pipistrelhis were infected. 2. In the blood of P. pipistrelhis, the parasite closely resembled T. (S.) cruzi. When grown in vitro in monophasic or diphasic media at 28°C, epimastigotes and trypomastigotes developed. The latter were of two types — very long, thin forms and less numerous shorter individuals. 3. The trypanosomes multiplied as amastigotes within HeLa and mouse L cells in vitro. After 6–9 days in HeLa cells at 37°C, they transformed into small trypomastigotes and emerged from the cells. Higher infection rates (up to about 4%) were obtained in cell cultures inoculated with flagellates from older monophasic cultures, which contained more of the long, slender trypomastigotes. 4. Trypanosomes also entered (or were phagocytosed by) up to 60% or more of mouse peritoneal macrophages in vitro. Multiplication occurred by binary and multiple fission of amastigotes in at least some of the parasitized macrophages and transformation into trypomastigotes was seen after 7 or more days at 37°C. Development in macrophages was less synchronous than in HeLa cells.

scholarly journals A Critical Role of Activin A in Maturation of Mouse Peritoneal Macrophages in vitro and in vivo

2009 ◽  
Vol 6 (5) ◽  
pp. 387-392 ◽  
Author(s):  
Yinan Wang ◽  
Xueling Cui ◽  
Guixiang Tai ◽  
Jingyan Ge ◽  
Nan Li ◽  
...  
Keyword(s):  
Critical Role ◽  
Peritoneal Macrophages ◽  
Activin A ◽  
Mouse Peritoneal Macrophages

scholarly journals Immunomodulatory Action of Triterpene Glycosides Isolated from the Sea Cucumber Actinocucumis typica. Structure-Activity Relationships

2014 ◽  
Vol 9 (6) ◽  
pp. 1934578X1400900 ◽  
Author(s):  
Evgeny A. Pislyagin ◽  
Dmitry L. Aminin ◽  
Alexandra S. Silchenko ◽  
Sergey A. Avilov ◽  
Pelageya V. Andryjashchenko ◽  
...  
Keyword(s):  
Peritoneal Macrophages ◽  
Triterpene Glycosides ◽  
Cytotoxic Activities ◽  
Ros Formation ◽  
Low Toxicity ◽  
Low Cytotoxicity ◽  
Immunomodulatory Action ◽  
Mouse Peritoneal Macrophages ◽  
Stimulation Of

Stimulation of lysosomal activity and ROS formation in mouse peritoneal macrophages by five triterpene glycosides, typicosides A1 (1), A2 (2), B1 (3), C1 (4) and C2 (5) has been studied and compared with their cytotoxic activities. Glycosides 1–3 possess moderate activities, but the most cytotoxic glycoside 5 is not active. Typicoside C1 (4), with low toxicity, was proved to be the most active concerning stimulation of ROS formation. This is the first example of a triterpene glycoside from sea cucumbers with low cytotoxicity, but which demonstrates a strong immunostimulatory effect on mouse peritoneal macrophages in vitro.

scholarly journals Colony formation in vitro by mouse blood monocytes

Blood ◽  
1977 ◽  
Vol 49 (4) ◽  
pp. 593-598 ◽  
Author(s):  
H Lin
Keyword(s):  
Gamma Irradiation ◽  
Colony Formation ◽  
Mononuclear Phagocytes ◽  
Blood Monocytes ◽  
Mouse Blood ◽  
L Cells

Abstract Mouse blood monocytes were induced to proliferate and form discrete colonies of mononuclear phagocytes in liquid culutre. The proliferation of these cells in vitro required a factor or factors present in medium conditioned by L cells. For this class of colony-forming cells, the value of Do to gamma irradiation in vitro was 195 rads.

Uptake of ionic 55Fe by mouse peritoneal macrophages in vitro

1975 ◽  
Vol 95 (2) ◽  
pp. 385-395 ◽  
Author(s):  
Martha E. Fedorko ◽  
Carmine Lanni
Keyword(s):  
Peritoneal Macrophages ◽  
Mouse Peritoneal Macrophages
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