scholarly journals Lyt-2-/Lyt 3- variants of a cloned cytolytic T cell line lack an antigen receptor functional in cytolysis.

1981 ◽  
Vol 153 (3) ◽  
pp. 595-604 ◽  
Author(s):  
D P Dialynas ◽  
M R Loken ◽  
A L Glasebrook ◽  
F W Fitch
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Line ◽  
T Lymphocyte ◽  
Antigen Receptor ◽  
Ethyl Methanesulfonate ◽  
Cytolytic T Lymphocyte ◽  
Ethyl Methanesulfonate Mutagenesis

To investigate the role of Lyt-2 and Thy-1 in cytolysis, we have generated, by ethyl methanesulfonate mutagenesis and selection, variants of the cloned cytolytic T lymphocyte line L3 that specifically lack either Lyt-2 or Thy-1. An analysis of these variants indicates that neither Lyt-2 nor Lyt-3 is responsible for the lethal hit, but suggests that Lyt-2 and/or Lyt-3 are required for an antigen receptor functional in cytolysis. The data also suggest that the expression of Lyt-3 on the cell surface is not independent of the expression of Lyt-2. Finally the data indicate the Thy-1 plays no role in cytolysis.

scholarly journals Endocytosis and recycling of the T3-T cell receptor complex. The role of T3 phosphorylation.

1987 ◽  
Vol 165 (4) ◽  
pp. 1141-1159 ◽  
Author(s):  
M S Krangel
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Cell Line ◽  
T Cell Receptor ◽  
Leukemic Cell ◽  
Cell Receptor ◽  
Intact Cells ◽  
Surface Molecules ◽  
Stable Populations

An assay has been developed to assess the dynamics of cell surface glycoproteins, in which neuraminidase digestion of intact cells is used to determine the fate of cell surface molecules initially labelled via lactoperoxidase-catalyzed iodination. This approach has been used to demonstrate the constitutive endocytosis and recycling of the T3-T cell receptor (T CR) complex on the human T leukemic cell line HPB-MLT. Stable populations of both phosphorylated and nonphosphorylated forms of the T3 gamma peptide have been identified in these cells. Whereas the former are constitutively endocytosed, the latter appear to be excluded from this pathway. The results presented indicate that T3 gamma phosphorylation may control the endocytosis and recycling of the T3-TCR complex on this cell line.

scholarly journals Association of a tetraspanin CD9 with CD5 on the T cell surface: role of particular transmembrane domains in the association

1999 ◽  
Vol 11 (12) ◽  
pp. 2043-2052 ◽  
Author(s):  
Kazuhito Toyo-oka ◽  
Yumi Yashiro-Ohtani ◽  
Cheung-Seog Park ◽  
Xu-Guang Tai ◽  
Kensuke Miyake ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Surface ◽  
Transmembrane Domains

Human Cytolytic T-Lymphocyte Clones and Their Function-Associated Cell Surface Molecules

1985 ◽  
pp. 559-573 ◽  
Author(s):  
Alan M. Krensky ◽  
Steven J. Mentzer ◽  
Julia L. Greenstein ◽  
Mary Crimmins ◽  
Carol Clayberger ◽  
...  
Keyword(s):  
Cell Surface ◽  
T Lymphocyte ◽  
Cell Surface Molecules ◽  
Surface Molecules ◽  
Cytolytic T Lymphocyte

scholarly journals MicroRNA and circRNA Expression Analysis in a Zbtb1 Gene Knockout Monoclonal EL4 Cell Line

2021 ◽  
Vol 11 ◽  
Author(s):  
Jun-Hong Wang ◽  
Chun-Wei Shi ◽  
Yi-Yuan Lu ◽  
Yan Zeng ◽  
Ming-Yang Cheng ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Line ◽  
Gene Deletion ◽  
Cell Lymphoma ◽  
Gene Knockout ◽  
Expression Profiles ◽  
T Cell Lymphoma ◽  
Differentially Expressed ◽  
Btb Domain

Zinc finger and BTB domain containing 1(Zbtb1) is a transcriptional suppressor protein, and a member of the mammalian Zbtb gene family. Previous studies have shown that Zbtb1 is essential for T-cell development. However, the role of Zbtb1 in T-cell lymphoma is undetermined. In this study, an EL4 cell line with Zbtb1 deletion was constructed using the CRISPR-Cas9 technique. The expression profiles of microRNA and circRNA produced by the control and gene deletion groups were determined by RNA-seq. In general, 24 differentially expressed microRNA and 16 differentially expressed circRNA were found between normal group and gene deletion group. Through further analysis of differentially expressed genes, GO term histogram and KEGG scatter plot were drawn, and three pairs of miRNA and circRNA regulatory relationships were found. This study describes the differentially expressed microRNA and circRNA in normal and Zbtb1-deficient EL4 cell lines, thus providing potential targets for drug development and clinical treatment of T-cell lymphoma.

scholarly journals The role of Raf-1 in the regulation of extracellular signal-regulated kinase 2 by the T cell antigen receptor.

1994 ◽  
Vol 180 (1) ◽  
pp. 401-406 ◽  
Author(s):  
M Izquierdo ◽  
S Bowden ◽  
D Cantrell
Keyword(s):  
T Cells ◽  
T Cell ◽  
Map Kinases ◽  
Interleukin 2 ◽  
Antigen Receptor ◽  
T Cell Antigen Receptor ◽  
Cell Antigen Receptor ◽  
Cell Antigen ◽  
Constitutively Active

Triggering of the T cell antigen receptor (TCR) complex activates the serine/threonine kinase Raf-1 whose function is necessary for TCR induction of the interleukin 2 gene. Raf-1 has been identified as a candidate mitogen-activated protein (MAP) kinase kinase kinase (MKKK) and thus has the potential to couple the TCR to the activation of the MAP kinases such as ERK2. In the present study, the role of Raf-1 in ERK2 regulation of ERK2 in T cells has been explored. A constitutively active Raf-1 kinase, v-raf, or a dominant inhibitory Raf-1 mutant were expressed transiently from the pEF BOS vector in Jurkat cells and the effects of these Raf-1 mutants on a coexpressed ERK2 reporter was assessed. The action of the constitutively active Raf-1 was to stimulate the ERK2 kinase, whereas the dominant negative version of Raf-1 inhibited the ERK2 activation induced by triggering of the TCR. These data indicate a role for Raf-1 in the regulation of ERK2 in T cells.

Alloreactive Cytolytic T Lymphocyte Precursor Cells in Aged C57B1 /6 nu/nu Mice: Frequency and Cell Surface Phenotype

2015 ◽  
pp. 21-24
Author(s):  
J. L. Maryanski ◽  
H. R. MacDonald ◽  
R. K. Lees ◽  
B. Sordat ◽  
J. -C. Cerottini
Keyword(s):  
Cell Surface ◽  
T Lymphocyte ◽  
Precursor Cells ◽  
Surface Phenotype ◽  
Cytolytic T Lymphocyte
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