scholarly journals Restricted immunoglobulin variable region gene usage by normal Ly-1 (CD5+) B cells that recognize phosphatidyl choline.

1989 ◽  
Vol 169 (6) ◽  
pp. 1869-1877 ◽  
Author(s):  
T J Mercolino ◽  
A L Locke ◽  
A Afshari ◽  
D Sasser ◽  
W W Travis ◽  
...  
Keyword(s):  
B Cells ◽  
Phosphatidyl Choline ◽  
Mammalian Cells ◽  
Variable Region ◽  
Normal Adult ◽  
Region Gene ◽  
Fragment Analysis ◽  
Gene Usage ◽  
Variable Region Genes ◽  
Immunoglobulin Variable Region Gene

5-15% of lymphocytes in the peritoneums of normal adult B10.H-2aH-4bp/Wts (2a4b) mice are CD5+ (Ly-1) B cells that recognize phosphatidyl choline (PtC), a phospholipid component of all mammalian cells. We produced a set of IgM-secreting hybridomas from the peritoneal cells of normal, adult 2a4b mice. We found that this set of hybridomas shows a similarly high frequency of antibodies specific for PtC (21 of 86) that also react with bromelain-treated mouse erythrocytes. Restriction fragment analysis of Ig gene rearrangements and analysis of expressed Ig idiotypes reveal that these cells use a restricted set of variable region genes to generate the PtC-specific antibodies. The Ig genes used by the PtC-specific hybridomas appear to be the same as those found in the PtC-specific Ly-1 B cell lymphomas, CH27 and CH34.

scholarly journals Clarifying immunoglobulin gene usage in systemic and localized immunoglobulin light-chain amyloidosis by mass spectrometry

Blood ◽  
2017 ◽  
Vol 129 (3) ◽  
pp. 299-306 ◽  
Author(s):  
Taxiarchis V. Kourelis ◽  
Surendra Dasari ◽  
Jason D. Theis ◽  
Marina Ramirez-Alvarado ◽  
Paul J. Kurtin ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Variable Region ◽  
Immunoglobulin Gene ◽  
Region Gene ◽  
Key Points ◽  
Gene Usage ◽  
Immunoglobulin Light Chain Amyloidosis ◽  
Tissue Specimens ◽  
Organ Tropism ◽  
Immunoglobulin Variable Region Gene

Key Points Mass spectrometry is a high-throughput, low-resource technique that can identify immunoglobulin variable region gene from tissue specimens. IGVL gene usage is restricted and different between systemic and localized AL and only partially explains organ tropism in this disease.

Biased immunoglobulin variable region gene expression by Ly-1 B cells due to clonal selection

1989 ◽  
Vol 19 (7) ◽  
pp. 1289-1295 ◽  
Author(s):  
Christopher A. Pennell ◽  
Thomas J. Mercolino ◽  
Therese A. Grdina ◽  
Larry W. Arnold ◽  
Geoffrey Haughton ◽  
...  
Keyword(s):  
Gene Expression ◽  
B Cells ◽  
Clonal Selection ◽  
Variable Region ◽  
Region Gene ◽  
Immunoglobulin Variable Region ◽  
Variable Region Gene ◽  
Immunoglobulin Variable Region Gene

Characterisation of the immunoglobulin variable region gene usage encoding the murine anti-ganglioside antibody repertoire

2005 ◽  
Vol 165 (1-2) ◽  
pp. 92-103 ◽  
Author(s):  
Judith Boffey ◽  
Masaaki Odaka ◽  
Dawn Nicoll ◽  
Eric R. Wagner ◽  
Kate Townson ◽  
...  
Keyword(s):  
Variable Region ◽  
Antibody Repertoire ◽  
Region Gene ◽  
Immunoglobulin Variable Region ◽  
Variable Region Gene ◽  
Gene Usage ◽  
Immunoglobulin Variable Region Gene

scholarly journals Intrinsic Constraint on Plasmablast Growth and Extrinsic Limits of Plasma Cell Survival

2000 ◽  
Vol 192 (6) ◽  
pp. 813-822 ◽  
Author(s):  
Daniel M.-Y. Sze ◽  
Kai-Michael Toellner ◽  
Carola García de Vinuesa ◽  
Dale R. Taylor ◽  
Ian C.M. MacLennan
Keyword(s):  
B Cells ◽  
Plasma Cell ◽  
Plasma Cells ◽  
Variable Region ◽  
Antibody Responses ◽  
Finite Capacity ◽  
Region Gene ◽  
Variable Region Gene ◽  
Immunoglobulin Variable Region Gene

B cells recruited into splenic antibody responses grow exponentially, either in extrafollicular foci as plasmablasts, or in follicles where they form germinal centers. Both responses yield plasma cells. Although many splenic plasma cells survive <3 d, some live much longer. This study shows that early plasma cell death relates to a finite capacity of the spleen to sustain plasma cells rather than a life span endowed by the cell's origin or the quality of antibody it produces. Antibody responses were compared where the peak numbers of plasma cells in spleen sections varied between 100 and 5,000 cells/mm2. In each response, plasmablast clones divided some five times, with the peak numbers of plasma cells produced relating directly to the number of B cells recruited into the response. The spleen seems to have the capacity to sustain between 20 and 100 plasma cells/mm2. When this number is exceeded, there is a loss of excess cells. Immunoglobulin variable region gene sequencing, and 5-bromo-2′-deoxyuridine pulse–chase studies indicate that long-lived splenic plasma cells are a mixture of cells derived from the extrafollicular and germinal center responses and cells derived from virgin and memory B cells. Only a proportion has switched immunoglobulin class.

Sign in / Sign up

Export Citation Format

Share Document