scholarly journals A 15 amino acid fragment of influenza nucleoprotein synthesized in the cytoplasm is presented to class I-restricted cytotoxic T lymphocytes.

1989 ◽  
Vol 170 (3) ◽  
pp. 1051-1056 ◽  
Author(s):  
K Gould ◽  
J Cossins ◽  
J Bastin ◽  
G G Brownlee ◽  
A Townsend
Keyword(s):  
Cell Clone ◽  
Recombinant Vaccinia Virus ◽  
Class I ◽  
Peptide Transport ◽  
Cytotoxic T Cell ◽  
Target Cells ◽  
Recombinant Vaccinia ◽  
Acid Fragment ◽  
T Cell Clone ◽  
Membrane Bound

A recombinant vaccinia has been designed to express amino acids 366-379 of influenza nucleoprotein, previously shown to be the minimal epitope recognized by a class I-restricted cytotoxic T cell clone. Target cells infected with the recombinant vaccinia virus expressing this peptide are recognized by CTL as efficiently as target cells expressing the complete nucleoprotein. The results imply the existence of a peptide transport system that constitutively passes the products of degraded proteins from the cytoplasm into a membrane-bound compartment of the cell.

scholarly journals CD8 cytotoxic T-cell clone rapidly transfers autoimmune diabetes in very young NOD and MHC class I-compatible scid mice

Diabetologia ◽  
1997 ◽  
Vol 40 (9) ◽  
pp. 1044-1052 ◽  
Author(s):  
R. Yoneda ◽  
K. Yokono ◽  
M. Nagata ◽  
Y. Tominaga ◽  
H. Moriyama ◽  
...  
Keyword(s):  
T Cell ◽  
Mhc Class I ◽  
Cell Clone ◽  
Autoimmune Diabetes ◽  
Scid Mice ◽  
Class I ◽  
Cytotoxic T Cell ◽  
T Cell Clone

An Ovalbumin Peptide-Specific Cytotoxic T Cell Clone with Antigen Self-Presentation Capacity Uses Two Distinct Mechanisms to Kill Target Cells

1993 ◽  
Vol 152 (2) ◽  
pp. 333-347 ◽  
Author(s):  
Thomas Dick ◽  
Gabi Reichmann ◽  
Klaus Ebnet ◽  
Markus M. Simon ◽  
Hans-Peter Dienes ◽  
...  
Keyword(s):  
T Cell ◽  
Cell Clone ◽  
Cytotoxic T Cell ◽  
Target Cells ◽  
Self Presentation ◽  
T Cell Clone

Characterization of MHC Class-I Restricted TCRαβ+CD4−CD8− Double-Negative T Cells Recognizing the gp100 Antigen from a Melanoma Patient after gp100 Vaccination

Blood ◽  
2008 ◽  
Vol 112 (11) ◽  
pp. 4905-4905
Author(s):  
Simon Voelkl ◽  
Tamson Moore ◽  
Michael Rehli ◽  
Michael Nishimura ◽  
Karin Fischer ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Mhc Class I ◽  
Peripheral Blood ◽  
Melanoma Patient ◽  
Cell Clone ◽  
Class I ◽  
Target Cells ◽  
Double Negative ◽  
T Cell Clone

Abstract The immune attack against malignant tumors requires the concerted action of CD8+ cytotoxic T lymphocytes (CTL) as well as CD4+ T helper cells. The contribution of T cell receptor (TCR)αβ+ CD4− CD8− double-negative (DN) T cells to anti-tumor immune responses is widely unknown. In previous studies, we have demonstrated that DN T cells with a broad TCR repertoire are present in humans in the peripheral blood and the lymph nodes of healthy individuals. Here we characterize a human DN T cell clone (T4H2) recognizing an HLA-A2-restricted melanoma-associated antigenic gp100-peptide isolated from the peripheral blood of a melanoma patient. Antigen recognition by the T4H2 DN clone resulted in specific secretion of IFN-γ and TNF. Although lacking the CD8 molecule the gp100-specifc DN T cell clone was able to confer antigen-specific cytotoxicity against gp100-loaded target cells as well as HLA-A2+ gp100 expressing melanoma cells. The cytotoxic capacity was found to be perforin/granzymeB-dependent. Together, these data indicate that functionally active antigen-specific DN T cells recognizing MHC class I-restricted tumor-associated antigen (TAA) may contribute to anti-tumor immunity in vivo.

scholarly journals Aggregation of MHC class I molecules on a CD8+α β T cell clone specifically inhibits non-antigen-specific lysis of target cells

2004 ◽  
Vol 34 (1) ◽  
pp. 47-55 ◽  
Author(s):  
Esther Caparrós ◽  
A. Beltrán de Heredia ◽  
Emilio Carpio ◽  
David Sancho ◽  
Enrique Aguado ◽  
...  
Keyword(s):  
T Cell ◽  
Mhc Class I ◽  
Cell Clone ◽  
Class I ◽  
Target Cells ◽  
Specific Lysis ◽  
T Cell Clone ◽  
Mhc Class I Molecules

scholarly journals T Cell Activity Correlates with Oligomeric Peptide-Major Histocompatibility Complex Binding on T Cell Surface

2001 ◽  
Vol 276 (50) ◽  
pp. 47320-47328 ◽  
Author(s):  
Jennifer Buslepp ◽  
Rui Zhao ◽  
Debora Donnini ◽  
Douglas Loftus ◽  
Mohamed Saad ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Cell Surface ◽  
Cell Clone ◽  
Cell Activity ◽  
Class I ◽  
Antigenic Peptides ◽  
Major Histocompatibility ◽  
Class I Mhc ◽  
T Cell Clone

Recognition of virally infected cells by CD8+T cells requires differentiation between self and nonself peptide-class I major histocompatibility complexes (pMHC). Recognition of foreign pMHC by host T cells is a major factor in the rejection of transplanted organs from the same species (allotransplant) or different species (xenotransplant). AHIII12.2 is a murine T cell clone that recognizes the xenogeneic (human) class I MHC HLA-A2.1 molecule (A2) and the syngeneic murine class I MHC H-2 Dbmolecule (Db). Recognition of both A2 and Dbare peptide-dependent, and the sequences of the peptides recognized have been determined. Alterations in the antigenic peptides bound to A2 cause large changes in AHIII12.2 T cell responsiveness. Crystal structures of three representative peptides (agonist, null, and antagonist) bound to A2 partially explain the changes in AHIII12.2 responsiveness. Using class I pMHC octamers, a strong correlation is seen between T cell activity and the affinity of pMHC complexes for the T cell receptor. However, contrary to previous studies, we see similar half-lives for the pMHC multimers bound to the AHIII12.2 cell surface.

scholarly journals A Class II-Restricted CD8γ13 T-Cell Clone Protects During Chlamydia muridarum Genital Tract Infection

2020 ◽  
Vol 221 (11) ◽  
pp. 1895-1906
Author(s):  
Raymond M Johnson ◽  
Norma Olivares-Strank ◽  
Gang Peng
Keyword(s):  
T Cells ◽  
T Cell ◽  
Mhc Class Ii ◽  
Cd8 T Cells ◽  
Genital Tract ◽  
Cell Clone ◽  
Class Ii ◽  
Class I ◽  
T Cell Clone ◽  
Tract Infections

Abstract Background The T-cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I restricted (referred to here as “unrestricted” or “atypical”). We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a cytokine polarization pattern that included interferon (IFN)-γ and interleukin (IL)-13. Methods In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. Results To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. Conclusions The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function.

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