STUDIES ON AN AGGLUTINOGEN (Rh) IN HUMAN BLOOD REACTING WITH ANTI-RHESUS SERA AND WITH HUMAN ISOANTIBODIES

Studies are reported on an individual agglutinogen (Rh) in human blood which has been found to be of clinical importance because occasionally it gives rise to the formation of immune isoantibodies in man, a peculiarity which leads to untoward transfusion reactions. A method for the determination of the presence or absence of the new blood factor is described, which can be used for typing patients and prospective blood donors. Examination of families showed that the agglutinogen is inherited as a simple Mendelian dominant. The distribution of the factor Rh among white individuals and negroes may indicate racial differences. The property is probably genetically independent of the blood groups and the factors M and N.

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BGvar - a comprehensive resource for blood group immunogenetics

10.1101/2021.02.04.429861 ◽

2021 ◽

Author(s):

Mercy Rophina ◽

Kavita Pandhare ◽

Sudhir Jadhao ◽

Shivashankar H. Nagaraj ◽

Vinod Scaria

Keyword(s):

Blood Group ◽

Human Blood ◽

Clinical Importance ◽

Blood Groups ◽

Widespread Application ◽

Human Blood Group ◽

Genomic Technologies ◽

Literature Evidence ◽

Public Datasets ◽

Safe Blood

AbstractBackgroundBlood groups form the basis of effective and safe blood transfusion. There are about 41 well recognized human blood group systems presently known. Blood groups are molecularly determined by the presence of specific antigens on the red blood cells and are genetically determined and inherited following Mendelian principles. The lack of a comprehensive, relevant, manually compiled and genome-ready dataset of red cell antigens limited the widespread application of genomic technologies to characterise and interpret the blood group complement of an individual from genomic datasets.Materials and MethodsA range of public datasets were used to systematically annotate the variation compendium for its functionality and allele frequencies across global populations. Details on phenotype or relevant clinical importance were collated from reported literature evidence.ResultsWe have compiled the Blood Group Associated Genomic Variant Resource (BGvar), a manually curated online resource comprising all known human blood group related allelic variants including a total of 1672 ISBT approved alleles and 1552 alleles predicted and curated from literature reports. This repository includes 1606 Single Nucleotide Variations (SNVs), 270 Insertions, Deletions (InDels) and Duplications and about 1310 combination mutations corresponding to 41 human blood group systems and 2 transcription factors. This compendium also encompasses gene fusion and rearrangement events occurring in human blood group genes.ConclusionTo the best of our knowledge, BGvar is a comprehensive and a user friendly resource with most relevant collation of blood group alleles in humans. BGvar is accessible online at URL: http://clingen.igib.res.in/bgvar/

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Determination of Plasminogen in Human Plasma by the Lysis Time Method

Thrombosis and Haemostasis ◽

10.1055/s-0038-1655622 ◽

1966 ◽

Vol 16 (01/02) ◽

pp. 001-017 ◽

Cited By ~ 8

Author(s):

W Berg ◽

K Korsan-Bengtsen ◽

J Ygge

Keyword(s):

Human Plasma ◽

Present Method ◽

Blood Donors ◽

Fibrinogen Concentration ◽

Plasmin Activity ◽

Time System ◽

Lysis Time ◽

High Dilutions ◽

Single Determination

SummaryA one-stage lysis time system containing fibrinogen, streptokinase, thrombin, and a known, small amount of plasminogen was used to determine plasminogen in plasma.The known amount of plasminogen was added to the system in order to keep the lysis times relatively short when a highly diluted plasma was tested. High dilutions of plasma were used to reduce the influence of the plasma inhibitors.The calculation of the plasminogen concentration was made on the basis of the correlation: “plasminogen = fibrinogen/lysis time” which was valid in the system. The method allowed determination of plasminogen in plasma with varying fibrinogen concentrations, as the fibrinogen concentration in plasma was considered in the calculation.The presence of “spontaneous” plasmin activity in the plasma did not influence the plasminogen determination. Estimated by this method, the plasminogen content in plasma from 32 blood donors aged 25-45 years was 13.1 ±2.4 casein u/ml. The error of a single determination was 0.3 casein u/ml. The plasminogen content in plasma, determined with the present method, is about 3-4 times higher than the content found when a caseinolytic method is used.

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STUDY OF DISTRIBUTION OF BLOOD GROUPS AND HAZARDS OF TRANSFUSION REACTIONS

International Journal of Engineering Applied Sciences and Technology ◽

10.33564/ijeast.2019.v04i05.039 ◽

2019 ◽

Vol 04 (05) ◽

pp. 259-266

Author(s):

Surbhi Rathore ◽

Dr. Vaibhav Sharma

Keyword(s):

Blood Groups ◽

Transfusion Reactions

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Determination of total protein in human blood by laser

Diyala Journal for Pure Science ◽

10.24237/djps.1303.peb2 ◽

2017 ◽

Vol 13 (3) ◽

pp. 261-274

Author(s):

Sahra Salem Jassem ◽

Keyword(s):

Human Blood ◽

Total Protein

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Determination of a new rapid plasmin inhibitor in human blood by means of a plasmin specific tripeptide substrate

Scandinavian Journal of Clinical and Laboratory Investigation ◽

10.3109/00365518209168420 ◽

1982 ◽

Vol 42 ◽

pp. 403-409

Author(s):

A. -C. Teger-Nilsson ◽

P. Friberger ◽

E. Gyzander

Keyword(s):

Human Blood ◽

Plasmin Inhibitor

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DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

International Journal of Clinical and Biomedical Research ◽

10.31878/ijcbr.2019.54.03 ◽

2019 ◽

pp. 10-13

Author(s):

Ranu Kumar ◽

Prasad Kapildeo

Keyword(s):

Human Blood ◽

Blood Cells ◽

Blood Smear ◽

Clinical Laboratory ◽

White Blood Cells ◽

Healthcare Services ◽

Field Level ◽

Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

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