DETERMINATION OF WHITE BLOOD CELLS USING FOLDSCOPE WITH SMARTPHONE

2019 ◽  
pp. 10-13
Author(s):  
Ranu Kumar ◽  
Prasad Kapildeo
Keyword(s):  
Human Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Healthcare Services ◽  
Field Level ◽  
Morphology Analysis

We are traditionally used Microscope in clinical laboratory for determination of white blood cells of human blood smear. Now, in this study we were used Foldscope with Smartphone in the place of Microscope and examine many samples of human blood smear which was collected from local diagnostic centers. We were very easily quantity & morphology analysis of all types of WBC cells such as Neutrophils, Lymphocytes, Monocytes, Eosionophils, Basophils in blood smear with the help of Foldscope & image taken by Smartphone. The main objective of this study is to use Foldscope for quantity & morphology analysis of human WBCs at field level especially poor resource area where healthcare services or centers is not available & where carry of microscope is not possible.

PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI

2018 ◽  
Vol 3 (2) ◽  
pp. 52-61
Author(s):  
Dzikra Arwie ◽  
Islawati
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Field Of View ◽  
Laboratory Observation ◽  
Number Of Cells

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.  

Penentuan Kriteria Penilaian Kesan Jumlah Leukosit Pada Pemeriksaan Apusan Darah Tepi

2018 ◽  
Vol 3 (2) ◽  
pp. 118-127
Author(s):  
Dzikra Arwie ◽  
Islawati
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Field Of View ◽  
Laboratory Observation ◽  
Number Of Cells

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.

scholarly journals Recognition of Infected Erythrocytes by Inclusion Tree Representation and Parasitemia Estimation in Blood Smear Images

2019 ◽  
pp. 308-312
Author(s):  
Doke Pranoti R. ◽  
Doke Pooja R
Keyword(s):  
Blood Cells ◽  
Blood Smear ◽  
Early Stage ◽  
White Blood Cells ◽  
Splitting Method ◽  
World Health ◽  
Tree Representation ◽  
Cell Stage ◽  
Health Organization

Blood cells are composed of erythrocytes (red blood cells, RBCs), leukocytes (white blood cells, WBCs) and thrombocytes (platelets). Both WBC and RBC have fixed count in our body. If their count is less than the ideal count then it is an indication that our body is not healthy. Hence blood count helps in detecting many diseases in early stage.</p> <p>According to World Health Organization about 3.2 billion people are at risk of malaria[2]. But, malaria is preventable and curable, if the patient is correctly diagnosed in early stage.</p> <p>The proposed approach to diagnose malaria mainly consists of following steps:</p> <ol> <li>Preprocessing, Histogram and Segmentation</li> <li>Inclusion-Tree representation</li> <li>Splitting of clumped erythrocytes</li> <li>Counting and labeling</li> <li>Cell stage identification</li> <li>Feature extraction and classification</li> </ol> <p>The algorithm is used to count malaria infected RBC in blood smear. A clump splitting method is used for precise RBC counting. Cell stage identification is performed in this approach by calculating Equivalent Circular Diameter. Quantification method improves overall performance in the determination of stages of infection such as ring, trophozoite and Schizont. Percentage of Parasitemia is calculate.

scholarly journals PENENTUAN KRITERIA PENILAIAN KESAN JUMLAH LEUKOSIT PADA PEMERIKSAAN APUSAN DARAH TEPI

2018 ◽  
Vol 3 (2) ◽  
pp. 118-127
Author(s):  
Dzikra Arwie ◽  
Islawati
Keyword(s):  
Data Analysis ◽  
Statistical Analysis ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Field Of View ◽  
Laboratory Observation ◽  
Number Of Cells

Leukocytes or white blood cells have a characteristic characteristic of different cells. Determination of the impression of the number of leukocytes is determined in the number of cells in the field of view. While the number of viewable field cells expressed is still quite varied. The purpose of this study was to determine the number of leukocytes in the field of view and expressed the impression of a sufficient amount. This research was conducted at the Laboratory of Health Analyst Department Panrita Husada Bulukumba on 9 April 2017 to 14 July 2017. This type of research is a laboratory observation that aims to determine the criteria for assessing the impression of the number of leukocytes on a peripheral blood smear. Data analysis using statistical analysis is the average and standard deviations to determine the impression of the number of leukocytes and use 3 inspection zones. The results of this study obtained results in zone IV the number of leukocyte impressions said to be sufficient was 7-10, in zone V the number of leukocyte impressions said to be sufficient was 4-9, and in zone VI the number of leukocyte impressions said to be sufficient was 3-8.

scholarly journals Blood Cell Count Using Deep Learning Semantic Segmentation

2019 ◽  
Author(s):  
Thanh Tran ◽  
Lam Binh Minh ◽  
Suk-Hwan Lee ◽  
Ki-Ryong Kwon
Keyword(s):  
Deep Learning ◽  
Blood Cell ◽  
Cell Count ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Semantic Segmentation ◽  
Peripheral Blood Smear ◽  
Cell Counting ◽  
Blood Cell Count

Clinically, knowing the number of red blood cells (RBCs) and white blood cells (WBCs) helps doctors to make the better decision on accurate diagnosis of numerous diseases. The manual cell counting is a very time-consuming and expensive process, and it depends on the experience of specialists. Therefore, a completely automatic method supporting cell counting is a viable solution for clinical laboratories. This paper proposes a novel blood cell counting procedure to address this challenge. The proposed method adopts SegNet - a deep learning semantic segmentation to simultaneously segment RBCs and WBCs. The global accuracy of the segmentation of WBCs, RBCs, and the background of peripheral blood smear images obtains 89% when segment WBCs and RBCs from the background of blood smear images. Moreover, an effective solution to separate grouped or overlapping cells and cell count is presented using Euclidean distance transform, local maxima, and connected component labeling. The counting result of the proposed procedure achieves an accuracy of 93.3% for red blood cell count using dataset 1 and 97.38% for white blood cell count using dataset 2.

Development of a robust algorithm for detection of nuclei of white blood cells in peripheral blood smear images

2019 ◽  
Vol 78 (13) ◽  
pp. 17879-17898 ◽  
Author(s):  
Roopa B. Hegde ◽  
Keerthana Prasad ◽  
Harishchandra Hebbar ◽  
Brij Mohan Kumar Singh
Keyword(s):  
Peripheral Blood ◽  
Blood Cells ◽  
Blood Smear ◽  
White Blood Cells ◽  
Peripheral Blood Smear ◽  
Robust Algorithm

scholarly journals Extraction of Nucleolus Candidate Zone in White Blood Cells of Peripheral Blood Smear Images Using Curvelet Transform

2012 ◽  
Vol 2012 ◽  
pp. 1-12 ◽  
Author(s):  
Ramin Soltanzadeh ◽  
Hossein Rabbani ◽  
Ardeshir Talebi
Keyword(s):  
Blood Cells ◽  
Main Part ◽  
Blood Smear ◽  
Active Form ◽  
White Blood Cells ◽  
Curvelet Transform ◽  
Peripheral Blood Smear ◽  
Important Symptom ◽  
Blast Cells ◽  
Giant Nuclei

The main part of each white blood cell (WBC) is its nucleus which contains chromosomes. Although white blood cells (WBCs) with giant nuclei are the main symptom of leukemia, they are not sufficient to prove this disease and other symptoms must be investigated. For example another important symptom of leukemia is the existence of nucleolus in nucleus. The nucleus contains chromatin and a structure called the nucleolus. Chromatin is DNA in its active form while nucleolus is composed of protein and RNA, which are usually inactive. In this paper, to diagnose this symptom and in order to discriminate between nucleoli and chromatins, we employ curvelet transform, which is a multiresolution transform for detecting 2D singularities in images. For this reason, at first nuclei are extracted by means ofK-means method, then curvelet transform is applied on extracted nuclei and the coefficients are modified, and finally reconstructed image is used to extract the candidate locations of chromatins and nucleoli. This method is applied on 100 microscopic images and succeeds with specificity of 80.2% and sensitivity of 84.3% to detect the nucleolus candidate zone. After nucleolus candidate zone detection, new features that can be used to classify atypical and blast cells such as gradient of saturation channel are extracted.

scholarly journals HISTOLOGY OF BONE LESION AND CORRELATION OF CLINICAL, CLINICAL-LABORATORY DATA AND MORPHOLOGIC INDICES IN DESTRUCTIVE FORM OF OSTEOMYELITIS WITH LATENT COURSE (BRODIE’S ABSCESS)

2018 ◽  
Vol 25 (2) ◽  
pp. 47-55
Author(s):  
V. V. Grigorovskiy ◽  
N. P. Gritsai ◽  
A. S. Gordiy ◽  
O. B. Lyutko ◽  
A. V. Grigorovskaya
Keyword(s):  
Blood Cells ◽  
Clinical Laboratory ◽  
White Blood Cells ◽  
Bone Lesion ◽  
Laboratory Data ◽  
Internal Capsule ◽  
Laboratory Methods ◽  
Brodie’S Abscess ◽  
Low Degree ◽  
Morphological Diagnosis

Urgency. Although the basic pathomorphologic features of bone lesion in destructive form of osteomyelitis with latent clinical course (Brodie’s abscess) are known from the literature, the thorough analytical clinical-morphologic studies have not been yet conducted.Purpose of study: Based on the results of the study to establish correlation dependence between clinical, clinical-laboratory indices and morphologic foci parameters.Material and methods. Fragments of pathologic foci tissues from 25 patients with Brodie’s abscess (31 samples) were the study material. The results of clinical, visualizing and clinical-laboratory methods as well as morphometric semi-quantitative indices characterizing the state of foci tissues were used for the gradationfrequency and correlation analysis.Results. In Brodie’s abscess the bone tissue morphologic changes are presented by spongiosa necrosis and resorption. More often the cases with granulation tissue within the internal capsule of a membrane prevailed. The evidence of exudative inflammation corresponded to the low degree of activity, of a productive one — to high activity. In the majority of cases small sequesters within the capsule tissues were detected. Reliable (p0.05) correlations that corresponded to absolute values of correlation coefficient ra in the range of 0.3 — 0.7 were determined for the following indices: “patient’s age” — “exudative inflammation within the capsule”, “white blood cells” — “pattern of capsule connective tissue”, “white blood cells” — “productive inflammation within the capsule”, “white blood cells” — “presence of sequesters”, “Antistaphylococcal antibodies” — “exudative inflammation within the capsule”.Conclusion. Determined clinical-morphologic correlations could be used to improve both clinical and morphological diagnosis of bone inflammatory lesions.

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