scholarly journals STUDIES ON PITUITARY LACTOGENIC HORMONE

1940 ◽  
Vol 23 (4) ◽  
pp. 433-438 ◽  
Author(s):  
Choh Hao Li ◽  
William R. Lyons ◽  
Herbert M. Evans

The Tiselius technique of electrophoresis has been used to study the homogeneity and mobility of pituitary lactogenic hormone. The lactogenic preparation employed by us shows only one sharp band in schlieren photographs, indicating a high degree of homogeneity. From the determination of the mobility of the hormone at different hydrogen ion concentrations in acetate and phosphate buffer of ionic strength 0.055, the isoelectric point was found to be 5.70 and the See PDF for Equation value 4.5 x 10–5. The difference in the mobility reported for White's crystalline preparation and that found with our high potency lactogenic preparation is discussed.

1995 ◽  
Vol 38 (1) ◽  
Author(s):  
R. Di Giovambattista ◽  
S. Barba ◽  
A. Marchetti

Automated amplitude response of the complete seismometer, telemetry and recording system js obtaiued trom sinusoidal inputs to the calibration coil. Custom-built software was designed to perform fully automatic cali- bration analyses of the digital signals. In this paper we describe the signals used for calibration and interactive and batch procedures designed to obtain calibration functions in automatic mode. By using a steady-state method we reach a high degree of accuracy in the determination of both the frequency and amplitude of the \ignal. The only parameters required by this procedure are the seismometer mass, the calibration-coil constant and the intensity of the current injected into the calibration coil. This procedure is applicable to telemetered seismic systems and represents an optimization of the processing time. The software was designed to requjre no modification" jf the device used to generate the sinusoidal current should change. In particular, it is possi- ble to changc the number of monotrequcncy packages transmitted to the calibration coil with the on]y restric- tion that the difference between the frequency of two consecutjve packages be greater than 5%; for these rea- sons the procedure is expected to be usefu] for the seismological community. The paper inc]udes a generaI de- scription of thc designing criteria, and of the hardware and software architecture, as well as an account of thc system's performancc during a two year period of operation.


1998 ◽  
Vol 14 (4) ◽  
pp. 749-756 ◽  
Author(s):  
Toru OZEKI ◽  
Yasuo TSUBOSAKA ◽  
Shizu NAKAYAMA ◽  
Nobuaki OGAWA ◽  
Takashi KIMOTO

1933 ◽  
Vol 16 (4) ◽  
pp. 637-655 ◽  
Author(s):  
Raymond C. Ingraham ◽  
Charles Lombard ◽  
Maurice B. Visscher

1. Calculations from the Fick diffusion law are shown to predict that membrane equilibria should be established during the course of ultrafiltration. 2. It is shown that the chloride ion is more concentrated and the sodium ion less concentrated in the ultrafiltrate than in the plasma from which the ultrafiltrate was derived. 3. It has been found that by increasing the base bound by protein through a reduction in the bicarbonate content the difference between the plasma concentration and the ultrafiltrate concentration for the several ions studied increases. 4. Calculations from the Donnan equation as to the magnitude of the change in base bound by protein at differing hydrogen ion concentrations are in substantial agreement with the observed values, thus rendering it probable that the membrane equilibrium effect is responsible for the change in distribution ratios observed. 5. It is pointed out that the observed difference in the distribution ratio of cations from that of the chloride anion is probably to be explained by the influence of protein in lowering the activity coefficient of cations when on the alkaline side of the isoelectric point. 6. It is pointed out that account must be taken of these observations in any consideration of the rôle of ultrafiltration in the production of any secretion or body fluid.


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