scholarly journals Alkaline Phosphatase Content and the Effects of Prednisolone on Mammalian Cells in Culture

1962 ◽  
Vol 45 (3) ◽  
pp. 439-485 ◽  
Author(s):  
Rody P. Cox ◽  
Colin M. MacLeod
Keyword(s):  
Alkaline Phosphatase ◽  
Mitotic Index ◽  
Cell Lines ◽  
Mammalian Cells ◽  
Culture Cell ◽  
Enzyme Concentration ◽  
Cell Protein ◽  
Cell Multiplication ◽  
Tissue Culture Cell ◽  
Similar Reduction

The alkaline phosphatase content of different tissue culture cell lines has been shown to vary from no detectable activity to high enzyme concentration. Within the epithelial lines studied alkaline phosphatase is either constitutive or inducible. Two epithelial cell strains in which alkaline phosphatase was "absent" could be induced to develop significant amounts of the enzyme when grown in the presence of Δ1-hydrocortisone. Phosphate did not repress enzyme induction by prednisolone. Under conditions of deadaptation the induced enzyme was diluted by cell multiplication. The mouse fibroblastic L line and several human fibroblastic lines did not contain alkaline phosphatase when grown under the conditions described nor could they be induced to produce the enzyme when cultivated in medium with prednisolone. Δ1-Hydrocortisone has other characteristic effects on established mammalian cell cultures which vary among cell lines. Human epithelial lines show reduction in cell multiplication with increase in mitotic index. The cytoplasm is increased and cell volume is nearly doubled. Mouse fibroblasts show a similar reduction in cell multiplication with a decrease in mitotic index. There is no increase in cell cytoplasm. Human fibroblast strains show no inhibition of multiplication or alteration in total cell protein when grown in medium containing prednisolone. Antisera prepared against "negative" prednisolone-inducible human cell lines and against a positive human line inhibited alkaline phosphatase activity to an equal degree.

Attachment of Salmonella to mammalian cells in vitro

1983 ◽  
Vol 29 (12) ◽  
pp. 1731-1735 ◽  
Author(s):  
Clifford S. Mintz ◽  
Dean O. Cliver ◽  
R. H. Deibel
Keyword(s):  
Cell Lines ◽  
Mammalian Cells ◽  
Culture Cell ◽  
Intestinal Cell ◽  
Tissue Culture Cell ◽  
Bacterial Cells ◽  
Intestinal Cell Line ◽  
Hela Cell Lines ◽  
High Concentrations

The attachment of Salmonella typhimurium strain PHL67342 to several mammalian tissue culture cell lines was investigated. Strain PHL67342 failed to attach in significant numbers to the Buffalo green monkey (BGM), swine testicular (ST), and HeLa cell lines. Significant attachment was observed with the Henle intestinal cell line. Log-phase cells of strain PHL67342 attached in greatest numbers to the Henle cells after 45 min of incubation at 37 °C. Attachment to the Henle cells was not affected by D-mannose or D-galactose, but was markedly inhibited by high concentrations of alpha-methyl-D-mannoside. Also, Salmonella lipopolysaccharide had no effect on the attachment of strain PHL67342 to the Henle cells. Fimbriae were not detected on the bacterial cells used in the adherence experiments. These results suggest that some bacterial factor(s) other than fimbriae and lipopolysaccharide mediate the attachment of strain PHL67342 to the Henle cells.

scholarly journals Use of Tissue Culture Cell Lines to Evaluate HIV Antiviral Resistance

2008 ◽  
Vol 24 (7) ◽  
pp. 957-967 ◽  
Author(s):  
Halina Krowicka ◽  
James E. Robinson ◽  
Rebecca Clark ◽  
Shannon Hager ◽  
Stephanie Broyles ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Antiviral Resistance ◽  
Tissue Culture Cell

The human ankyrin-1 gene is selectively transcribed in erythroid cell lines despite the presence of a housekeeping-like promoter

Blood ◽  
2000 ◽  
Vol 96 (3) ◽  
pp. 1136-1143 ◽  
Author(s):  
Patrick G. Gallagher ◽  
Marc Romana ◽  
William T. Tse ◽  
Samuel E. Lux ◽  
Bernard G. Forget
Keyword(s):  
Cell Lines ◽  
Transcription Initiation ◽  
Gene Promoter ◽  
Housekeeping Gene ◽  
Culture Cell ◽  
Erythroid Cell ◽  
Tissue Culture Cell ◽  
Mobility Shift ◽  
Genes Encoding

To begin to study the sequence variations identified in the 5′ flanking genomic DNA of the ankyrin gene in ankyrin-deficient hereditary spherocytosis patients and to provide additional insight into our understanding of the regulation of genes encoding erythrocyte membrane proteins, we have identified and characterized the erythroid promoter of the human ankyrin-1 gene. This compact promoter has characteristics of a housekeeping gene promoter, including very high G+C content and enzyme restriction sites characteristic of an HTF-island, no TATA, InR, or CCAAT consensus sequences, and multiple transcription initiation sites. In vitro DNAseI footprinting analyses revealed binding sites for GATA-1, CACCC-binding, and CGCCC-binding proteins. Transfection of ankyrin promoter/reporter plasmids into tissue culture cell lines yielded expression in erythroid, but not muscle, neural, or HeLa cells. Electrophoretic mobility shift assays, including competition and antibody supershift experiments, demonstrated binding of GATA-1, BKLF, and Sp1 to core ankyrin promoter sequences. In transfection assays, mutation of the Sp1 site had no effect on reporter gene expression, mutation of the CACCC site decreased expression by half, and mutation of the GATA-1 site completely abolished activity. The ankyrin gene erythroid promoter was transactivated in heterologous cells by forced expression of GATA-1 and to a lesser degree BKLF.

Growth of primate and nonprimate tissue culture cell lines in X-irradiated and cortisone-treated rats

Cancer ◽  
1958 ◽  
Vol 11 (6) ◽  
pp. 1236-1241 ◽  
Author(s):  
Lewis L. Coriell ◽  
Robert M. McAllister ◽  
Bernard M. Wagner ◽  
Sheldon R. Wilson ◽  
Selena A. Dwight
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Tissue Culture Cell

New tissue culture cell lines derived from human squamous cell carcinoma of the cervix and vagina

1978 ◽  
Vol 130 (4) ◽  
pp. 487-496 ◽  
Author(s):  
J.C. Porter ◽  
Richard H. Nalick ◽  
Frank Vellios ◽  
William B. Neaves ◽  
P.C. MacDonald
Keyword(s):  
Tissue Culture ◽  
Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Cell Lines ◽  
Squamous Cell ◽  
Culture Cell ◽  
Tissue Culture Cell ◽  
Human Squamous Cell Carcinoma

scholarly journals Utilization of the TangoTM β-Arrestin Recruitment Technology for Cell-Based EDG Receptor Assay Development and Interrogation

2009 ◽  
Vol 14 (9) ◽  
pp. 1134-1141 ◽  
Author(s):  
Justin A. Wetter ◽  
Chetana Revankar ◽  
Bonnie J. Hanson
Keyword(s):  
Tissue Culture ◽  
Cell Lines ◽  
Culture Cell ◽  
Assay Development ◽  
Receptor Expression ◽  
Tissue Culture Cell ◽  
Functional Assay ◽  
Entire Family ◽  
Cellular Assay ◽  
Receptor Signal

Cellular assay development for the endothelial differentiation gene (EDG) family of G-protein-coupled receptors (GPCRs) and related lysophospholipid (LP) receptors is complicated by endogenous receptor expression and divergent receptor signaling. Endogenously expressed LP receptors exist in most tissue culture cell lines. these LP receptors, along with other endogenously expressed GPCRs, contribute to off-target signaling that can complicate interpretation of second-messenger-based cellular assay results. these receptors also activate a diverse and divergent set of cellular signaling pathways, necessitating the use of a variety of assay formats with mismatched procedures and functional readouts. this complicates examination and comparison of these receptors across the entire family. the tango™ technology uses the conserved β-arrestin-dependent receptor deactivation process to allow interrogation of the EDG and related receptors with a single functional assay. this method also isolates the target receptor signal, allowing the use of tissue culture cell lines regardless of their endogenous receptor expression. the authors describe the use of this technique to build cell-based receptor-specific assays for all 8 members of the EDG receptor family as well as the related LPA receptors GPR23, GPR92, and GPR87. In addition, they demonstrate the value of this technology for identification and investigation of functionally selective receptor compounds as demonstrated by the immunosuppressive compound FtY720-P and its action at the EDG1 and EDG3 receptors. ( Journal of Biomolecular Screening 2009:1134-1141)

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