scholarly journals Use of the Polymerase Chain Reaction for the Detection of Legionella pneumophila DNA in Serum Samples

1997 ◽  
Vol 25 (4) ◽  
pp. 939-939 ◽  
Author(s):  
Peggy Matsiota‐Bernard ◽  
Georgia Vrioni ◽  
Charles Nauciel
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Chain Reaction ◽  
Serum Samples ◽  
Polymerase Chain

scholarly journals Application of polymerase chain reaction for detection of Legionella pneumophila in serum samples

1998 ◽  
Vol 4 (3) ◽  
pp. 144-148 ◽  
Author(s):  
S. Alexiou-Daniel ◽  
A. Stylianakis ◽  
A. Papoutsi ◽  
I. Zorbas ◽  
A. Papa ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Chain Reaction ◽  
Serum Samples ◽  
Polymerase Chain

Subtyping ofLegionella pneumophilaisolates by arbitrarily primed polymerase chain reaction

1995 ◽  
Vol 41 (9) ◽  
pp. 846-848 ◽  
Author(s):  
E. Ledesma ◽  
J. Llorca ◽  
M. A. Dasí ◽  
M. L. Camaró ◽  
E. Carbonell ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Water Sources ◽  
Chain Reaction ◽  
Single Room ◽  
Legionnaires Disease ◽  
Polymerase Chain ◽  
Resort Hotel ◽  
Different Water Sources

Arbitrarily primed polymerase chain reaction (AP-PCR) was used to differentiate strains of Legionella pneumophila isolated from different water sources in a resort hotel in Benidorm, Alicante, Spain, where an outbreak of Legionnaires' disease occurred among a group of tourists between 65 and 80 years of age. All isolates were L. pneumophila serogroup 1, subtype Pontiac (Knoxville 1). Five different patterns (P1 to P5) were obtained by AP-PCR. The number of bands per pattern varied between 4 and 11. Patterns P1 and P2 represented 60 and 20% of L. pneumophila isolates, respectively. Since different subpopulations of L. pneumophila coexisted (up to three different AP-PCR patterns were identified in a single room), it was not possible to link an individual L. pneumophila strain to the occurrence of this outbreak.Key words: Legionella pneumophila, AP-PCR, subtyping, outbreak.

scholarly journals Study of Legionella pneumophila Detection by Two Step Polymerase Chain Reaction

1993 ◽  
Vol 67 (11) ◽  
pp. 1062-1067
Author(s):  
Michio KOIDE ◽  
Atsushi SAITO ◽  
Futoshi HIGA ◽  
Yuuko YAMASHIRO ◽  
Tomohiko ISHIMINE ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Legionella Pneumophila ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Step Polymerase Chain Reaction

scholarly journals Evaluation of the Reverse Transcription-Polymerase Chain Reaction/Probe Test of Serum Samples and Immunohistochemistry of Skin Sections for Detection of Acute Bovine Viral Diarrhea Infections

2002 ◽  
Vol 14 (4) ◽  
pp. 303-307 ◽  
Author(s):  
Julia F. Ridpath ◽  
Sharon K. Hietala ◽  
Steve Sorden ◽  
John D. Neill
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Amplification ◽  
Clinical Signs ◽  
Bovine Viral Diarrhea ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Serum Samples ◽  
Viral Diarrhea ◽  
Persistent Infections ◽  
Polymerase Chain

Bovine viral diarrhea viruses (BVDV) cause both acute and persistent infections. While diagnostic tests have been designed to detect animals persistently infected (PI) with BVDV, the reliability of these tests in detecting acute BVDV infections is not known. It is also possible that acute BVDV infections may be confused with persistent infections in surveys for PI animals. In this study, 2 tests presently in use in diagnostic laboratories to test for PI animals, polymerase chain reaction amplification followed by probe hybridization (RT-PCR/probe) of serum samples and immunohistochemical detection of viral antigen in skin biopsies (IHC), were evaluated for their ability to detect acute BVDV infections. Sixteen colostrum-deprived, BVDV-free, and BVDV-antibody-free calves were infected with 6 different BVDV strains. Clinical signs, seroconversion, and virus isolation indicated that inoculated animals did replicate virus. Virus could be detected in 19% (3/16) of acutely infected animals by the RT-PCR/probe technique. No acutely infected animals were positive by IHC.

Molecular Detection of Leptospira Species Serotypes in Iranian Stray Dogs

2019 ◽  
Author(s):  
Saam Torkan ◽  
Hassan Momtaz
Keyword(s):  
Public Health ◽  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Serum Samples ◽  
Blood Samples ◽  
Stray Dogs ◽  
Wide Range ◽  
Leptospira Spp ◽  
Polymerase Chain ◽  
Leptospira Species

Background and Aims: Leptospirosis is a spirochetal disease with public health importance globally. This disease affects a wide range of domestic and wild animals. Dogs are one of the species most sensitive to Leptospira canicola and Leptospira icterohaemorrhagiae. The present study was concluded to evaluate the prevalence rate of Leptospira species and L. canicola and L. icterohaemorrhagiae serovars in Iranian stray dogs. Materials and Methods: One-hundred and twenty blood samples were first taken from stray dogs. Then the samples were transferred to the laboratory. Sera were extracted from blood samples and genomic DNA was extracted. DNA samples were subjected to conventional polymerase chain reaction. Positive samples for Leptospira spp. were analyzed for presence of L. canicola and L. icterohaemorrhagiaeserovars using the polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Results: Nine samples out of 120 serum samples (7.5%) were positive for the flagella gene of the Leptospira spp. Prevalence of Leptospira spp. in serum samples of male and female dogs were 5.4% and 10.86%, respectively. Prevalence of L. canicola and L. icterohaemorrhagiae serovars were 55.55% and 33.33%, respectively. We found that 11.11% of samples were positive for both serovars. Two to three and 3-4 year old dogs had the highest prevalence of Leptospira spp. Conclusions: The considerable prevalence of leptospirta spp. and also their zoonotic serovars among Iranian stray dogs represented an important public health issue regarding the contact of healthy human with these dogs. Identification of infected dogs and their vaccination can inhibit the distribution of Leptospira spp.

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