Dual-crosslinked 3D printed gelatin scaffolds with potential for temporomandibular joint cartilage regeneration

2021 ◽  
Author(s):  
Espen Helgeland ◽  
Ahmad Rashad ◽  
Elisabetta Campodoni ◽  
Oyvind Goksoyr ◽  
Torbjørn Østvik Pedersen ◽  
...  
Keyword(s):  
Temporomandibular Joint ◽  
Cartilage Regeneration ◽  
Joint Cartilage ◽  
3D Printed

3D printed gelatin-genipin scaffolds for temporomandibular joint cartilage regeneration

2021 ◽  
Author(s):  
Espen Helgeland ◽  
Samih Mohamed Ahmed ◽  
Siddharth Vivek Shanbhag ◽  
Torbjørn Østvik Pedersen ◽  
Annika Rosén ◽  
...  
Keyword(s):  
Temporomandibular Joint ◽  
Cartilage Regeneration ◽  
Joint Cartilage ◽  
3D Printed

scholarly journals Lithium Chloride-Releasing 3D Printed Scaffold for Enhanced Cartilage Regeneration

2019 ◽  
Vol 25 ◽  
pp. 4041-4050 ◽  
Author(s):  
Jiayi Li ◽  
Qingqiang Yao ◽  
Yan Xu ◽  
Huikang Zhang ◽  
Liang-liang Li ◽  
...  
Keyword(s):  
Lithium Chloride ◽  
Cartilage Regeneration ◽  
3D Printed

scholarly journals Composite System of 3D-Printed Polymer and Acellular Matrix Hydrogel to Repair Temporomandibular Joint Disc

2021 ◽  
Vol 8 ◽  
Author(s):  
Ping Yi ◽  
Jiadi Liang ◽  
Futing Huang ◽  
Zuodong Zhao ◽  
Xiaohui Zhou ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Temporomandibular Joint ◽  
Photoelectron Spectroscopy ◽  
Biomechanical Testing ◽  
Biomechanical Properties ◽  
Biological Properties ◽  
Cell Counting ◽  
Structural And Functional Properties ◽  
Tmj Disc ◽  
3D Printed

Tissue engineering is a promising approach to restore or replace a damaged temporomandibular joint (TMJ) disc. However, constructing a scaffold that can mimic biomechanical and biological properties of the natural TMJ disc remains a challenge. In this study, three-dimensional (3D) printing technology was used to fabricate polycaprolactone (PCL)/polyurethane (PU) scaffolds and PU scaffolds to imitate the region-specific biomechanical properties of the TMJ disc. The scaffolds were coated with polydopamine (PDA) and combined with a decellularized matrix (dECM). Then, rat costal chondrocytes and mouse L929 fibroblasts, respectively, were suspended on the composite scaffolds and the biological functions of the cells were studied. The properties of the scaffolds were characterized by scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS), contact angle analysis, and biomechanical testing. To verify the biocompatibility of the scaffolds, the viability, proliferation, and extracellular matrix (ECM) production of the cells seeded on the scaffolds were assessed by LIVE/DEAD staining, Cell Counting Kit-8 assay, biochemical content analysis, immunofluorescence staining, and qRT-PCR. The functionalized hybrid scaffolds were then implanted into the subcutaneous space of nude mice for 6 weeks, and the regenerated tissue was evaluated by histological staining. The biomechanical properties of PCL/PU and PU scaffolds were comparable to that of the central and peripheral zones, respectively, of a native human TMJ disc. The PDA-coated scaffolds displayed superior biomechanical, structural, and functional properties, creating a favorable microenvironment for cell survival, proliferation, ECM production, and tissue regeneration. In conclusion, 3D-printed polymer scaffolds coated with PDA and combined with dECM hydrogel were found to be a promising substitute for TMJ disc tissue engineering.

scholarly journals Tissue Engineered Vascularized Patient-Specific Temporomandibular Joint Reconstruction in a Yucatan Pig Model

2020 ◽  
Author(s):  
Shelly Abramowicz ◽  
Sarah Jo Crotts ◽  
Scott J. Hollister ◽  
Steve Goudy
Keyword(s):  
Temporomandibular Joint ◽  
Porcine Model ◽  
Superficial Temporal Artery ◽  
Mandibular Condyle ◽  
Temporal Artery ◽  
Patient Specific ◽  
Temporalis Muscle ◽  
Control Side ◽  
Titanium Screws ◽  
3D Printed

ABSTRACTPurposePediatric temporomandibular joint (TMJ) reconstruction occurs as a result of acquired, developmental, and/or congenital conditions. Current pediatric reconstruction options are limited. The aim of this project was to develop a proof of principle porcine model for a load bearing, customized 3-dimensional (3D) printed and BMP2-coated scaffold implanted in a pedicled (temporal) flap as a regenerative approach to pediatric TMJ mandibular condyle reconstruction.Materials and MethodsScaffolds were custom designed and 3D printed based on porcine computed tomography and absorbed with BMP2. Two operations occured: (1) implantation of scaffold in temporalis muscle to establish vascularity, and six weeks later, (2) unilateral condylectomy and rotation of the vascularized scaffold (with preservation of superficial temporal artery) onto mandibular ramus defect and secured with titanium screws. At 6 months post-implantation, the pigs were sacrified. The experimental side (muscle-scaffold) and the control side (unoperated condyle) were individually harvested at 6 months and evaluated by clinical, mechanical, radiographic, and qualitative/histologic methods.ResultsScaffolds maintained physical properties similar in appearance to unoperated condyles. The vascularized scaffolds had bone formation at edges and adjacent to scaffold-bone interface. New bone was visible in scaffold. Condyle height on the reconstructed side was 68% and 78% of the control side. Reconstructed condyle stiffness was between 20% and 45% of the control side.ConclusionIn our porcine model, customized 3D printed TMJ scaffolds impregnanted with BMP2 and implanted in and pedicled on temporalis muscle has the ability to (1) reconstruct a TMJ defect model, (2) maintain appropriate condylar height and upper airway diameter, and (3) generate new bone, without impacting functional outcomes.

Regeneration of Knee Joint Cartilage under the Effect of Low-Molecular Fraction of Cord Blood (up to 5 kDa)

2011 ◽  
Vol 18 (3) ◽  
pp. 69-74
Author(s):  
A K Gulevskiy ◽  
Evgeniy Gennadievich Ivanov ◽  
A K Gulevsky ◽  
Ye G Ivanov
Keyword(s):  
Knee Joint ◽  
Motor Activity ◽  
Cord Blood ◽  
Intramuscular Injection ◽  
Cartilage Regeneration ◽  
Structural Proteins ◽  
Joint Cartilage ◽  
Injured Limb ◽  
Molecular Fraction ◽  
Positive Effect

The effect of the cattle cord blood low-molecular fraction (up to 5 kDa) (FCB) on regeneration of knee joint cartilage following mechanical injury was studied in rats using biochemical, histological and roentgenological methods. It was shown that intramuscular injection of FCB significantly stimulated the accumulation of the basic components of matrix, the most important structural proteins in cartilage regeneration as well as provided the positive effect on morphologic state of cartilage and surrounding tissues. That positive effect was noted during both stage of granulation and stage of chondrocyte development. It was shown that injection of FCB promoted normalization of motor activity in injured limb.

Mesenchymal Stromal Cell Transplantation Induces Regeneration of Large and Full-Thickness Cartilage Defect of the Temporomandibular Joint

Cartilage ◽  
2020 ◽  
pp. 194760352092671 ◽  
Author(s):  
Marcos Gomez ◽  
Olga Wittig ◽  
Dylana Diaz-Solano ◽  
José E. Cardier
Keyword(s):  
Temporomandibular Joint ◽  
Subchondral Bone ◽  
Histological Analysis ◽  
Cellular Therapy ◽  
Platelet Rich Plasma ◽  
Articular Surface ◽  
Cartilage Damage ◽  
Cartilage Regeneration ◽  
Osteochondral Lesions ◽  
Field Evidence

Objective Cartilage damage (CD) in the temporomandibular joint (TMJ) continues being a major problem in maxillofacial field. Evidence suggests that cellular therapy may be used for repairing CD in the TMJ. Design A murine model of condyle CD (CCD) was generated in the TMJ to evaluate the capacity of mesenchymal stromal cells (MSCs) to induce cartilage regeneration in CCD. A large CCD was surgically created in a condyle head of the TMJ of C57BL/6 mice. Human MSC embedded into preclotted platelet-rich plasma (PRP) were placed on the surface of CCD. As controls, untreated CCD and exposed TMJ condyle (sham) were used. After 6 weeks, animals were sacrificed, and each mandibular condyle was removed and CCD healing was assessed macroscopically and histologically. Results Macroscopic observation of CCD treated with MSC showed the presence of cartilage-like tissue in the CCD site. Histological analysis showed a complete repair of the articular surface with the presence of cartilage-like tissue and subchondral bone filling the CCD area. Chondrocytes were observed into collagen and glycosaminoglycans extracellular matrix filling the repaired tissue. There was no evidence of subchondral bone sclerosis. Untreated CCD showed denudated osteochondral lesions without signs of cartilage repair. Histological analysis showed the absence of tissue formation over the CCD. Conclusions Transplantation of MSC induces regeneration of TMJ-CCD. These results provide strong evidence to use MSC as potential treatment in patients with cartilage lesions in the TMJ.

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