A functional extracellular matrix biomaterial enriched with VEGFA and bFGF as vehicle of human umbilical cord mesenchymal stem cells in skin wound healing

2021 ◽  
Author(s):  
Zhongjuan Xu ◽  
Junjun Cao ◽  
Zhe Zhao ◽  
Yong Qiao ◽  
Xingzhi Liu ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Extracellular Matrix ◽  
Mesenchymal Stem Cells ◽  
Umbilical Cord ◽  
Limited Resource ◽  
Host Cells ◽  
Endothelial Differentiation ◽  
Human Umbilical Cord ◽  
Skin Wound Healing

Abstract The construction of microvascular network is one of the greatest challenges for tissue engineering and cell therapy. Endothelial cells are essential for the construction of network of blood vessels. However, their application meets challenges in clinic due to the limited resource of autologous endothelium. Mesenchymal stem cells (MSCs) can effectively promote the angiogenesis in ischemic tissues for their abilities of endothelial differentiation and paracrine, and abundant sources. Extracellular matrix (ECM) has been widely used as an ideal biomaterial to mimic cellular microenvironment for tissue engineering due to its merits of neutrality, good biocompatibility, degradability, and controllability. In this study, a functional cell derived ECM biomaterial enriched with VEGFA and bFGF by expressing the collagen-binding domain (CBD) fused factor genes in host cells was prepared. This material could induce endothelial differentiation of human umbilical cord mesenchymal stem cells (hUCMSCs) and promote angiogenesis, which may improve the healing effect of skin injury. Our research not only provides a functional ECM material to inducing angiogenesis by inducing endothelial differentiation of hUCMSCs, but also shed light on the ubiquitous approaches to endow ECM materials different functions by enriching different factors. This study will greatly benefit tissue engineering and regenerative medicine researches.

scholarly journals Repair of Osteochondral Defects Using Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells in a Rabbit Model

2017 ◽  
Vol 2017 ◽  
pp. 1-12 ◽  
Author(s):  
Shuyun Liu ◽  
Yanhui Jia ◽  
Mei Yuan ◽  
Weimin Guo ◽  
Jingxiang Huang ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Umbilical Cord ◽  
Wharton’S Jelly ◽  
Cartilage Defects ◽  
Human Umbilical Cord ◽  
Wharton's Jelly

Umbilical cord Wharton’s jelly-derived mesenchymal stem cell (WJMSC) is a new-found mesenchymal stem cell in recent years with multiple lineage potential. Due to its abundant resources, no damage procurement, and lower immunogenicity than other adult MSCs, WJMSC promises to be a good xenogenous cell candidate for tissue engineering. This in vivo pilot study explored the use of human umbilical cord Wharton’s jelly mesenchymal stem cells (hWJMSCs) containing a tissue engineering construct xenotransplant in rabbits to repair full-thickness cartilage defects in the femoral patellar groove. We observed orderly spatial-temporal remodeling of hWJMSCs into cartilage tissues during repair over 16 months, with characteristic architectural features, including a hyaline-like neocartilage layer with good surface regularity, complete integration with adjacent host cartilage, and regenerated subchondral bone. No immune rejection was detected when xenograft hWJMSCs were implanted into rabbit cartilage defects. The repair results using hWJMSCs were superior to those of chondrogenically induced hWJMSCs after assessing gross appearance and histological grading scores. These preliminary results suggest that using novel undifferentiated hWJMSCs as seed cells might be a better approach than using transforming growth factor-β-induced differentiated hWJMSCs for in vivo tissue engineering treatment of cartilage defects. hWJMSC allografts may be promising for clinical applications.

scholarly journals Layer by layer deposited osteoinductive scaffolds for bone tissue engineering

2020 ◽  
Vol 9 (2) ◽  
pp. 1089-1098
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bone Tissue Engineering ◽  
Bone Tissue ◽  
Umbilical Cord ◽  
Human Umbilical Cord ◽  
Layer By Layer ◽  
Cissus Quadrangularis ◽  
Culture Extract

There are various bone regenerative and repair methods, but the use of osteoinductive scaffolds as bone grafts/substitute has gained wide importance worldwide. Here, an osteoinductive scaffold is developed which spontaneously stimulates stem cells to osteoblast formation without the use of any growth factor or differentiation media. We prepared electrospun PCL scaffold which is further modified for osteoinductivity by layer-by-layer method using graphene and Cissus quadrangularis callus culture extract (PCL-GP-CQ). The modified PCL-GP-CQ scaffold was compared with plain PCL scaffold and PCL coated only with GP. Physical properties such as roughness, wettability, yield strength and tensile strength of PCL-GP-CQ scaffold were found to be superior. Also, PCL-GP-CQ scaffold when seeded with human umbilical cord Wharton's jelly derived mesenchymal stem cells showed higher in vitro biocompatibility with enhanced cellular proliferation on its surface. Synergistic effect of graphene and Cissus Quadrangularis callus culture extract in scaffold boosted the differentiation of human Umbilical Cord Wharton’s jelly derived Mesenchymal Stem Cells into osteogenic lineage without any differentiation media in less than 20 days. The PCL-GP-CQ scaffold enhanced osteoblastic differentiation, osteoconduction and osteoinduction potential of scaffolds making them highly suitable for bone regeneration and bone tissue engineering applications.

scholarly journals A simple and scalable method to generate spheroids from human mesenchymal stem cells for use in tissue engineering

2020 ◽  
Vol 7 (12) ◽  
pp. 4139-4151
Author(s):  
Ngoc Bich Vu ◽  
Minh Thi-Nguyet Nguyen
Keyword(s):  
Tissue Engineering ◽  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Stem Cell ◽  
Umbilical Cord ◽  
Human Mesenchymal Stem Cells ◽  
Human Adipose Tissue ◽  
Necrotic Core ◽  
Porous Scaffolds ◽  
Human Umbilical Cord

Introduction: Tissue engineering is a field suited for applying stem cells, besides stem cell transplantation. In the current tissue engineering approaches, stem cells are typically seeded onto a suitable scaffold and induced into specific tissues under particular conditions. However, this strategy has faced some limitations, namely that stem cell proliferation on the scaffolds' surface has been inefficient to fill the porous scaffolds to produce solid tissues. Some limitations have been improved by using stem cell spheroids on the scaffold in place of single stem cells. This study aimed to evaluate a simple and feasible method to produce spheroids of mesenchymal stem cells (MSCs) from adipose and umbilical cord tissues for use in tissue engineering. Methods: MSCs from human adipose tissue (adipose-derived stem cells, i.e., ADSCs) and human umbilical cord tissues (umbilical cord-derived mesenchymal stem cells, i.e., UCMSCs) were isolated according to previously published protocols. To produce spheroids, ADSCs and UCMSCs were cultured in non-adherent V-bottom 96-well plate. Three cell densities were evaluated: 250 cells/well, 500 cells/well, and 1,000 cells/well. The generated spheroids were evaluated based on spheroid diameter, necrotic core formation (using propidium iodide (PI) and Hoechst 33342 staining), and spheroid structure (by Hematoxylin & Eosin staining). Results: The results showed that at a density of 250 cells/well, spheroids were formed without necrotic cores from both ADSCs and UCMSCs. However, at a higher density, all spheroids had a necrotic core as part of the three zones (proliferating, quiescent, and necrotic zones). Conclusion: Spheroids from ADSCs and UCMSCs can be easily produced by culturing 250 cells/well in a non-adherent V-bottom 96-well plate. This process can be scaled up by using the liquid handling robot system to load cells into the plates.

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