scholarly journals Black rice mutant strain selection results of M3 generation mutation breeding

2020 ◽  
Vol 486 ◽  
pp. 012094
Author(s):  
M M Putra ◽  
M Riadi ◽  
R Sjahril
Keyword(s):  
Mutant Strain ◽  
Mutation Breeding ◽  
Strain Selection ◽  
Black Rice ◽  
Rice Mutant

scholarly journals Mutation breeding for improvement of aromatic rice mutant by using ion beam irradiation

2020 ◽  
Vol 486 ◽  
pp. 012091
Author(s):  
A M Okasa ◽  
M Riadi ◽  
K Toriyama ◽  
K. Ishii ◽  
Y. Hasyashi ◽  
...  
Keyword(s):  
Ion Beam ◽  
Mutation Breeding ◽  
Aromatic Rice ◽  
Beam Irradiation ◽  
Ion Beam Irradiation ◽  
Rice Mutant

Mutation Breeding of Yeast Strains for Fig Fruit Wine

2013 ◽  
Vol 781-784 ◽  
pp. 1353-1358
Author(s):  
Yong Zuo ◽  
Li Ping Liu ◽  
Shuai Ju ◽  
Hui Xie ◽  
Peng Jiang ◽  
...  
Keyword(s):  
Mutant Strain ◽  
Microwave Treatment ◽  
Mutation Breeding ◽  
Original Strain ◽  
Uv Treatment ◽  
Wine Production ◽  
Natural Fermentation ◽  
Yeast Strains ◽  
Fruit Wine ◽  
Good Taste

Yeast Z20 which screened from natural fermentation fig fruit wine as the original strain, through the 80s UV treatment and 25s microwave treatment, mutant strain UV-5 were selected. Its produced ethanol 13.6%, and it has fast fermentation speed, high wine production degree and the residual sugar quantity is little. After subculture 9 times, the results showed that the UV-5 strain has good genetic stability. Combined with the sensory evaluation results, the wine which fermentation by the strain UV-5 has high clarity, good flavor and good taste, so that the mutant strain UV-5 suitable for fermentation fig fruit wine as special yeast.

Application of mutation breeding techniques in the development of green crop varieties in Sri Lanka: the way forward.

2021 ◽  
pp. 76-82
Author(s):  
Malathy Parasuraman ◽  
Priyantha Weerasinghe
Keyword(s):  
Sri Lanka ◽  
Genetic Variability ◽  
Gamma Rays ◽  
Mutation Breeding ◽  
Iron Toxicity ◽  
Mutant Line ◽  
High Yield ◽  
Rice Mutant ◽  
The 1960S ◽  
The Way

Abstract The Department of Agriculture (DOA) in Sri Lanka initiated mutation breeding in the 1960s with the introduction of a cobalt-60 source. The first rice mutant variety, MI 273, was released for general cultivation in 1971. M1 273, derived from irradiation of the H-4 variety, was identified as a drought-tolerant variety. An indirect rice mutant variety, developed by crossing the short mutant line BW267-3 with a highly adaptable variety, was released as BW 372 in 2013. It is moderately tolerant to blast, bacterial leaf blight, brown plant hopper, gall midge and iron toxicity, and thus increases productivity to 3-4 t/ha on lands prone to iron toxicity. The most popular groundnut variety cultivated in the country, 'Tissa', is a mutant developed by irradiation with gamma-rays at 200 Gy. It showed attributes of high yield, medium maturity (90-100 days) and high oil content (42%). 'Tissa' presently covers 80% of the groundnut cultivated area in Sri Lanka. A sesame mutant line, derived from the variety MI-3 irradiated at 200 Gy with 60Co gamma-rays, was released as 'Malee' (ANK-S2) in 1993. It is a high-yielding variety (1.1-1.8 t/ha) resistant to Phytophthora blight. A cherry-type mutant tomato variety, developed by irradiation of seeds with gamma-rays (320 Gy), was released as 'Lanka Cherry' in 2010. Improved attributes are pear-shaped fruits and bacterial wilt resistance. Narrow genetic variability in many crops is a constraint to the development of new varieties adapted to the changing climate. Hence, the DOA is emphasizing integration of induced mutagenesis in conventional breeding programmes to develop resistant/tolerant varieties having high yield, quality and health-promoting functional properties in field and horticultural crops. The newly installed gamma irradiation chamber facilitates the creation of genetic variability in food crops, thus paving the way for the development of greener varieties.

Impact of cross-breeding on the metabolites of the low phytic acid rice mutant Os-lpa-MH86-1.

2021 ◽  
pp. 433-443
Author(s):  
Chen-guang Zhou ◽  
Yuan-yuan Tan ◽  
Sophia Gossner ◽  
You-fa Li ◽  
Qing-yao Shu ◽  
...  
Keyword(s):  
Phytic Acid ◽  
Seed Viability ◽  
Mutation Breeding ◽  
Cross Breeding ◽  
Low Phytic Acid ◽  
Rice Mutant ◽  
Transporter Family ◽  
Independent Field ◽  
Food And Feed ◽  
The Stability

Abstract Phytic acid (myo-inositol-1,2,3,4,5,6-hexakisphosphate), the major storage form of phosphorus in cereals, is considered as an antinutrient in food and feed. During the past few years, various cereals have been subjected to mutation breeding for generating low phytic acid (lpa) crops. Recently, it was demonstrated that reduction of phytic acid in the rice mutant Os-lpa-MH86-1 obtained by gamma irradiation was due to a disruption of OsSULTR3;3, an orthologue of the sulfate transporter family group 3 genes. The application of a GC/MS-based metabolite profiling approach revealed that the reduction of phytic acid was accompanied by changes in concentrations of metabolites from different classes in the Os-lpa-MH86-1 mutant.Lpa mutant lines often exhibit lower grain yield and seed viability compared with their wild-type parents. To improve the agronomic performance of the Os-lpa-MH86-1 mutant, cross-breeding with a commercial cultivar was performed. The resulting progenies were genotyped using molecular markers to identify homozygous wildtype and lpa mutants from generations F4 to F7. The objectives of this study were: (i) to observe the consistent metabolic changes in Os-lpa-MH86-1 lpa mutants by following their composition over several independent field trials; (ii) to investigate the impact of cross-breeding on the phytic acid content and the metabolic phenotype of the homozygous lpa mutant; and (iii) to assess the stability of the mutation-specific metabolite signature in the lpa progenies over several generations. Statistical assessment of the data via multivariate and univariate approaches demonstrated that the lpa trait and the mutation-induced metabolite signature in the lpa progenies were comparable to the progenitor Os-lpa-MH86-1 mutant and consistently expressed over generations. These findings extend the basis for implementing mutation breeding in the generation of lpa rice cultivars.

scholarly journals A Convenient Assay for Estimating the Possible Involvement of Efflux of Fluoroquinolones by Streptococcus pneumoniae andStaphylococcus aureus: Evidence for Diminished Moxifloxacin, Sparfloxacin, and Trovafloxacin Efflux

2000 ◽  
Vol 44 (3) ◽  
pp. 798-801 ◽  
Author(s):  
Rebecca Beyer ◽  
Ekaterina Pestova ◽  
John J. Millichap ◽  
Valentina Stosor ◽  
Gary A. Noskin ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Streptococcus Pneumoniae ◽  
Mutant Strain ◽  
Resistant Mutant ◽  
Strain Selection ◽  
Hydrophobic Drug ◽  
Enhancing Effect

ABSTRACT We developed a simplified assay for estimating efflux by measuring the effect of reserpine on the growth of Streptococcus pneumoniae and Staphylococcus aureus over 7 h. Reserpine enhanced ciprofloxacin and levofloxacin 17 to 68%. The hydrophobic drug trovafloxacin and the drug moxifloxacin, with a bulky C-7 substituent but hydrophilicity similar to that of levofloxacin, showed little (0 to 11%) reserpine-enhancing effect. The ease of resistant mutant strain selection correlated with efflux susceptibility.

scholarly journals Plasma Mutation Breeding of High Yield γ-Aminobutyric Acid Lactic Acid Bacteria

2018 ◽  
Vol 1 (1) ◽  
Author(s):  
Gao Chengcheng
Keyword(s):  
Mutant Strain ◽  
Room Temperature ◽  
Hormone Secretion ◽  
Growth Hormone Secretion ◽  
Mutation Breeding ◽  
High Yield ◽  
Protein Amino Acid ◽  
Temperature Plasma ◽  
Amino Butyric Acid ◽  
Stable Mutant

γ-amino butyric acid (GABA) is a non-protein amino acid widely distributed in nature. It has analgesic effect,promotes growth hormone secretion and prevents the physiological function of Alzheimer's disease.In order toimprove the yield of GABA, the strain of strain HX-3-6 was tested by Atmospheric and Room Temperature Plasma(ARTP), and the strain with more than 90% lethality was selected for fermentation, and through the gradient plateaccording to the level of GABA concentration and colony size of the screening. Then, use the fermentation mediumfor re-screening and genetic stability test. The yield of the mutant strain L-120-1 was 5.828 g/L, which was 18.84%higher than that before the mutation (4.904). The mutant strain L-120-1 was used as the starting strain for ARTPmutagenesis. The yield of the stable mutant strain 90s-high was 6.178g / L, which was 16.15% higher than that beforethe mutation (5.319g / L). The yield of HX-3-6 strain was higher than that of GABA mutant strain 90s-high 2, whichwas stable and high yield GABA was 25.98%.

Anthocyanin-rich extract from black rice inhibits colon cancer formation in mice

2010 ◽  
Vol 34 (8) ◽  
pp. S44-S44
Author(s):  
Bo Dong ◽  
Xinmei Zhou ◽  
Xun Xu ◽  
Huang Xu ◽  
Yongxia Zheng ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Black Rice

EFFECT OF NICOTINE ON SEXUAL BEHAVIOUR OF MUTANT STRAIN (CURLED) OF DROSOPHILLA MELANOGASTER

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
SHAMIM AKHTER CHOUDHARY
Keyword(s):  
Mutant Strain ◽  
Sexual Behaviour ◽  
Virgin Female ◽  
P Value ◽  
Pooled Data ◽  
Mating Latency ◽  
Males And Females ◽  
Food Medium ◽  
Food Media ◽  
Dose Dependent

In the present study, an attempt was made to study the effect of plant extract on Sexual behaviour of Mutant Strain (Curled) of Drosophila melanogaster. The LC50 has been estimated with 1% of the food media. The virgin females and males were isolated and fed with normal food media for three days. Then sub-lethal concentrations of 0.625 μl / 100 ml food, 1.2 μl /100 ml food, 2.5μl /100 / food of nicotine were mixed in food medium and allowed in flies to feed for two days. Then appropriate combination of untreated / treated males and females were introduced into the mating chamber. Courtship latency, mating latency and copulation duration were studied. After observation of the behaviour, mated flies were allowed to produce progeny. The sexual behaviour of bachelor male and virgin female obtained in the progeny was also studied. The pooled data were analyzed by student t-test and the result indicates p-value significant at 0.05 levels. The courtship latency was affected by in treatment but it is neither dose dependent nor sex dependent.

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