scholarly journals Embryogenic callus induction of coffee [Coffea arabica L.] on several plant growth regulator concentration and incubation temperature

2020 ◽  
Vol 497 ◽  
pp. 012012
Author(s):  
R B Setiawan ◽  
M Rahmah ◽  
H Trisnia ◽  
I Chaniago ◽  
L Syukriani ◽  
...  
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Coffea Arabica ◽  
Incubation Temperature ◽  
Embryogenic Callus Induction ◽  
Coffea Arabica L

scholarly journals EFFECT OF DIFFERENT SOURCES OF PLANT GROWTH REGULATOR ON THE INDUCTION AND DEVELOPMENT OF MANGOSTEEN SOMATIC EMBRYOS

2017 ◽  
Vol 17 (1) ◽  
pp. 9
Author(s):  
Yosi Zendra Joni ◽  
Riry Prihatini ◽  
Darda Efendi ◽  
Ika Roostika
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Growth ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Somatic Embryos ◽  
Casein Hydrolysate ◽  
Malt Extract ◽  
Mass Propagation ◽  
Embryogenic Callus Induction

<p>Somatic embryogenesis is a technique for regenerating embryos derived from somatic cells of various plant species. This technique along with the utilization of plant growth regulator (PGR) might benefit for mass propagation and improvement of plant species through biotechnological tools. The study aimed to determine the effect of different plant growth regu-lators, namely 6-benzyladenine (BA) and thidiazuron (TDZ) on the embryogenic callus induction as well as casein hydrolysate and malt extract on the somatic embryo development of mangosteen. The explants used were in vitro young stems of mangosteen clone Leuwiliang. This study consisted of two experiments, namely induction of embryogenic callus and formation of somatic embryo. The first experiment was arranged as factorial in a completely randomized design with BA (0 and 0.7 mg l-1) as the first factor and TDZ (0, 0.1, 0.5 and 1.0 mg l-1) as the second factor. The second experiment consisted of four treatments, i.e. casein hydrolysate and malt extract at the rate of 500 and 1,000 mg l-1. The results showed that the best medium for embryogenic callus induction was MS supplemented with 0.1 mg l-1 TDZ, which resulted semifriable calli. Casein hydrolysate and malt extract could not induce the formation of somatic embryos. After two times subcultures on the same MS medium supplemented with 0.5 mg l-1 TDZ and 0.7 mg l-1 BA, a total of 33.8 somatic embryos per explant was induced. The successful somatic embryogenesis would support mangosteen breeding and in vitro mass propagation program.</p>

scholarly journals Organogenesis Kelapa Sawit (Elaeis guineensis Jacq.) Asal Eksplan Bunga Betina

2016 ◽  
Vol 2 (2) ◽  
pp. 104
Author(s):  
Tyas Larasati ◽  
Suci Rahayu ◽  
Fauziyah Harahap
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Callus Induction ◽  
Plant Growth Regulator ◽  
Elaeis Guineensis ◽  
Female Flower ◽  
Dry Weight ◽  
Flower Plant ◽  
Elaeis Guineensis Jacq ◽  
Dichlorophenoxy Acetic Acid

The objectives of this research were to composed organ from callus culture and to found the best concentration of plant growth regulator for organ growth from female flower explant of oil palm. This research has already done from June 2014 to May 2015 at Laboratory of Plant Physiology and Tissue Culture Department of Biology Faculty of Mathematics and Science University of North Sumatera. This research used Nonfactorial Completely Random Design. Explant was treated with five concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D; 99, 110, 120, 132, and 140 mg/L) for callus induction on Y3 medium (Eeuwens 1976). The result of this research showed that organ was formed from this treatment (basal segment of female flower explant) was root organ. 2,4-D plant growth regulator positively affected to growing of the root. The best result for time of callus induction, time of root growth, the highest percentage of explants that formed the root, fresh weight and dry weight of callus that has become the root generation was resulted from 99 mg/L 2,4-D.   Key words: Elaeis guineensis Jacq., female flower, plant growth regulator 2,4-D, organogenesis

scholarly journals Effects of genotype and plant growth regulators on callus induction in leaf cultures of Coffea arabica L. F1 hybrid

2019 ◽  
Vol 18 (31) ◽  
pp. 1004-1015
Author(s):  
W. I. Mwaniki ◽  
A. H. Lubabali ◽  
K. K. Asava ◽  
C. O. Agwanda ◽  
S. E. Anami
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Callus Induction ◽  
Coffea Arabica ◽  
F1 Hybrid ◽  
Coffea Arabica L

scholarly journals Peningkatan Induksi Kalus Embriogenik dan Konversi Embrio Somatik Kopi Robusta Klon BP 308

2017 ◽  
Vol 4 (3) ◽  
pp. 121
Author(s):  
Meynarti Sari Dewi Ibrahim ◽  
RR. Sri Hartati
Keyword(s):  
Tissue Culture ◽  
Plant Growth ◽  
Growth Regulator ◽  
Embryogenic Callus ◽  
Plant Growth Regulator ◽  
Somatic Embryos ◽  
Callus Formation ◽  
Fresh Weight ◽  
Robusta Coffee ◽  
Randomized Design

<em>Robusta coffee </em>(Coffea canephora)<em> is a cross-pollinated plant, therefore vegetative propagation is necessary to ensure identical traits with parents, such as tissue culture techniques through somatic embryo. The study aimed to find the effect of plant growth regulator 2.4-D and thidiazuron in inducing embryogenic callus, by adding incision area on leaf explant, and to evaluate addition of GA<sub>3</sub> in increasing somatic embryo conversion. The study was conducted from December 2014 to June 2016 in the Tissue Culture Laboratory, IAARD, Bogor. The research consisted of 2 stages. Stage 1 used a complete randomized design of 2 factors; the first factor was a combination of plant growth regulator 2.4-D (1.0 and 2.0 mg/l) and thidiazuron (1.0; 3.0; and 5.0 mg/l), the second factor was leaf incision (slashed and unslashed). Stage 2 used a complete randomized design, with GA<sub>3 </sub>treatment at different concentrations (0.0; 0.5; and 1.0 mg/l). Observed variables were percentage of callus formation, fresh weight of callus, number of torpedoes, number of somatic embryos at cotyledon stage, and number of germinated embryo. The results showed growth regulatory treatments influenced the percentage of embryogenic callus formation and fresh weight of callus. Extra incision on leaf showed no effect in  embryogenic callus induction. Embryogenic callus inducted using 2.4-D 1.0 mg/l + thidiazuron 5.0 mg/l medium which  then regenerated in ½ MS medium added with kinetin 2 mg/l exhibited  the highest number of germination.  Adding GA<sub>3</sub> 0.1 mg/l in regeneration medium is recommended to increase somatic embryos of Robusta coffee BP 308 clone.</em>

Influence of plant growth regulator and rhizobial inoculation on nodulation and soybean nitrogen contant

2007 ◽  
Vol 35 (2) ◽  
pp. 993-996 ◽  
Author(s):  
Sulejman Redžepović ◽  
Sanja Sikora ◽  
Josip Čolo ◽  
Mihaela Blažinkov ◽  
Marija Pecina
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Plant Growth Regulator ◽  
Rhizobial Inoculation
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