The amplification and analysis of cytochrome oxidase 1 (CO1) Gene of Ornithoptera croesus from Bacan Island

Ochthebius (Ochthebius) lobiccoastal habitatsollis Rey, 1885 is recorded for the first time from the Iberian Peninsula (Girona) and the island of Corsica; new records are also given for the islands of Menorca and Sardinia. The species is known only from coastal habitats through the Gulf of Lion and the Ligurian and Balearic seas, typically living in rockpools of different salinity or small trickles or freshwater runoffs. Genetic data of the cytochrome oxidase 1 gene from Iberian, Menorcan and Sardinian specimens shows less than 1% divergence, suggesting lack of isolation between populations.

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Genetic diversity within Scorpio maurus (Scorpiones: Scorpionidae) from morocco: Preliminary evidence based on CO1 mitochondrial DNA sequences

Biologia ◽

10.2478/s11756-008-0176-y ◽

2008 ◽

Vol 63 (6) ◽

Cited By ~ 11

Author(s):

Elsa Froufe ◽

Pedro Sousa ◽

Paulo Alves ◽

David Harris

Keyword(s):

Genetic Diversity ◽

Mitochondrial Dna ◽

Cytochrome Oxidase ◽

Dna Sequences ◽

Preliminary Analysis ◽

Mitochondrial Gene ◽

Sequence Divergence ◽

Preliminary Evidence ◽

Evidence Based ◽

Cytochrome Oxidase 1

AbstractThe large-clawed scorpion, Scorpio maurus, is a medically important scorpion and yet nothing is known regarding genetic diversity within this species. As a preliminary analysis we determined variation within the cytochrome oxidase 1 (CO1) mitochondrial gene from specimens from Morocco. High levels of genetic diversity were found that presented some geographical coherence. Of the two identified subspecies from Morocco, S. maurus birulai and S. maurus fuliginosus, the latter included genetically distinct lineages (8.0% uncorrected sequence divergence), indicating a detailed morphological and molecular revision is needed for this species.

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Barcoding, phylogeography and species boundaries in clownfishes of the Indian Ocean

DNA Barcodes ◽

10.1515/dna-2015-0002 ◽

2015 ◽

Vol 3 (1) ◽

Cited By ~ 3

Author(s):

Kottila Veettil Dhaneesh ◽

Thipramalai Thankappan Ajith Kumar ◽

Appukuttannair Biju Kumar

Keyword(s):

Indian Ocean ◽

Cytochrome Oxidase ◽

Phylogenetic Relationships ◽

Species Boundaries ◽

Cytochrome Oxidase 1 ◽

The Indian Ocean

AbstractIn this study, barcoding of 13 clownfish species of the Indian Ocean was carried out to infer the phylogenetic relationships among them by analyzing cytochrome oxidase 1 (CO1) and cytochrome

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KERAGAMAN GENETIK IKAN TIGER FISH (Datnioides sp.) ASAL KALIMANTAN DAN SUMATERA

Jurnal Riset Akuakultur ◽

10.15578/jra.13.3.2018.191-199 ◽

2018 ◽

Vol 13 (3) ◽

pp. 191

Author(s):

Melta Rini Fahmi ◽

Erma Primanita Hayuningtyas ◽

Mochammad Zamroni ◽

Bastiar Nur ◽

Shofihar Sinansari

Keyword(s):

Genetic Diversity ◽

Genetic Distance ◽

Cytochrome Oxidase ◽

Dna Barcoding ◽

Cytochrome B ◽

Molecular Identification ◽

Coi Gene ◽

Cyt B ◽

Cytochrome Oxidase 1 ◽

Rag2 Gene

Ikan tiger fish (Datnioides sp.) merupakan ikan hias air tawar yang memiliki nilai ekonomis penting. Distribusi populasi ikan ini meliputi Papua, Kalimantan, dan Sumatera, dengan tingkat eksploitasi yang cukup tinggi di dua lokasi terakhir. Penelitian ini dilakukan untuk mendapatkan informasi keragaman genetik ikan tiger fish yang mendiami perairan Kalimantan dan Sumatera. Sebanyak 24 sampel ikan uji dikoleksi dari Sungai Kapuas, Kalimantan Barat dan Sungai Musi, Sumatera Selatan. Penelitian dilakukan dalam dua tahap, tahap pertama yaitu identifikasi molekuler dengan menggunakan DNA barcoding gen cytochrome oxidase 1 (COI), tahap kedua adalah analisis keragaman genetik dengan menggunakan marka DNA mitokondria gen cytochrome b (Cyt b), dan DNA inti gen recombination activating gene (RAG2). Hasil identifikasi secara molekuler menunjukkan bahwa ikan hasil koleksi memiliki kesamaan genetik sebesar 100% dengan spesies D. undecimradiatus. Keragaman genetik ikan tiger fish antar populasi berkisar pada nilai 0,023 (standar deviasi 0,001) sedangkan keragaman intra populasi adalah sebesar 0,002 dan 0,003 masing-masing untuk populasi Kalimantan dan Sumatera. Jarak genetik sampel baik yang berasal dari Sumatera maupun Kalimantan dengan spesies D. undeciumradiatus masing-masing 0,003 dan 0,006; sedangkan dengan spesies D. microlepis yaitu 0,142. Analisis menggunakan gen RAG2 menunjukkan sampel yang diuji memiliki struktur populasi yang terpisah ditandai dengan terjadinya mutasi pada enam nukleotida dan tiga asam amino.The Tiger fish (Datnioides sp.) is a freshwater ornamental fish that has important economic value. The distribution of this fish included Papua, Kalimantan, and Sumatra, but intensive exploitation occurs in the last two population. This research was conducted to obtain the genetic diversity of tiger fish that inhabited in Kalimantan and Sumatra. A total of 24 fish were collected from Kapuas River, West Kalimantan and Musi River, at Sumatra. The study was conducted in two stages, the first stage is molecular identification of sample by using DNA barcoding cytochrome oxidase 1 (COI) gene, the second stage is analyses of genetic diversity of tiger fish within and between population by using the mitochondrial DNA cytochrome b (Cyt b) gene, and nucleus DNA recombination (RAG2) gene. The molecular identification has shown that the collected fish has a genetic similarity of 100% with D. undecimradiatus. The genetic diversity of tiger fish between populations is 0.023 (standard deviation of 0.001) whereas intra-population is 0.002 and 0.003 for Kalimantan and Sumatra, respectively. The genetic distance of samples with species D. undeciumradiatus were 0.003 and 0.006 for Kalimantan and Sumatera, respectively, whereas the genetic distance with D. microlepis was 0.142. The analysis of mutation on RAG2 gene shows there are six nucleotides and three amino acids have mutation.

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Caribbean Bulimulus revisited: physical moves and molecular traces (Mollusca, Gastropoda, Bulimulidae)

10.7287/peerj.preprints.1716 ◽

2016 ◽

Author(s):

Abraham SH Breure

Keyword(s):

Cytochrome Oxidase ◽

Species Delimitation ◽

Molecular Study ◽

Rapid Identification ◽

Distribution Range ◽

Cytochrome Oxidase 1 ◽

Comprehensive Database

Twenty-five samples of Bulimulus species are studied, partly from localities within their known distribution range, partly based on interceptions where the material originates from localities where the species seem to be recently introduced and non-native. Molecular study of cytochrome oxidase 1 (CO1) reveals the origin of some of these introductions, but is less conclusive for others. Four different methods for species delimitation were applied, which did not result in unambiguous species hypotheses. For a rapid identification of morphologically indistinct species, a more comprehensive database of sequences is needed.

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