Analysis of nucleotide diversity at the cytochrome b and cytochrome oxidase 1 genes at the population, species, and genus levels

2006 ◽  
Vol 42 (4) ◽  
pp. 341-362 ◽  
Author(s):  
Y. P. Kartavtsev ◽  
J. -S. Lee
Keyword(s):  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Nucleotide Diversity ◽  
Cytochrome Oxidase 1

scholarly journals KERAGAMAN GENETIK IKAN TIGER FISH (Datnioides sp.) ASAL KALIMANTAN DAN SUMATERA

2018 ◽  
Vol 13 (3) ◽  
pp. 191
Author(s):  
Melta Rini Fahmi ◽  
Erma Primanita Hayuningtyas ◽  
Mochammad Zamroni ◽  
Bastiar Nur ◽  
Shofihar Sinansari
Keyword(s):  
Genetic Diversity ◽  
Genetic Distance ◽  
Cytochrome Oxidase ◽  
Dna Barcoding ◽  
Cytochrome B ◽  
Molecular Identification ◽  
Coi Gene ◽  
Cyt B ◽  
Cytochrome Oxidase 1 ◽  
Rag2 Gene

Ikan tiger fish (Datnioides sp.) merupakan ikan hias air tawar yang memiliki nilai ekonomis penting. Distribusi populasi ikan ini meliputi Papua, Kalimantan, dan Sumatera, dengan tingkat eksploitasi yang cukup tinggi di dua lokasi terakhir. Penelitian ini dilakukan untuk mendapatkan informasi keragaman genetik ikan tiger fish yang mendiami perairan Kalimantan dan Sumatera. Sebanyak 24 sampel ikan uji dikoleksi dari Sungai Kapuas, Kalimantan Barat dan Sungai Musi, Sumatera Selatan. Penelitian dilakukan dalam dua tahap, tahap pertama yaitu identifikasi molekuler dengan menggunakan DNA barcoding gen cytochrome oxidase 1 (COI), tahap kedua adalah analisis keragaman genetik dengan menggunakan marka DNA mitokondria gen cytochrome b (Cyt b), dan DNA inti gen recombination activating gene (RAG2). Hasil identifikasi secara molekuler menunjukkan bahwa ikan hasil koleksi memiliki kesamaan genetik sebesar 100% dengan spesies D. undecimradiatus. Keragaman genetik ikan tiger fish antar populasi berkisar pada nilai 0,023 (standar deviasi 0,001) sedangkan keragaman intra populasi adalah sebesar 0,002 dan 0,003 masing-masing untuk populasi Kalimantan dan Sumatera. Jarak genetik sampel baik yang berasal dari Sumatera maupun Kalimantan dengan spesies D. undeciumradiatus masing-masing 0,003 dan 0,006; sedangkan dengan spesies D. microlepis yaitu 0,142. Analisis menggunakan gen RAG2 menunjukkan sampel yang diuji memiliki struktur populasi yang terpisah ditandai dengan terjadinya mutasi pada enam nukleotida dan tiga asam amino.The Tiger fish (Datnioides sp.) is a freshwater ornamental fish that has important economic value. The distribution of this fish included Papua, Kalimantan, and Sumatra, but intensive exploitation occurs in the last two population. This research was conducted to obtain the genetic diversity of tiger fish that inhabited in Kalimantan and Sumatra. A total of 24 fish were collected from Kapuas River, West Kalimantan and Musi River, at Sumatra. The study was conducted in two stages, the first stage is molecular identification of sample by using DNA barcoding cytochrome oxidase 1 (COI) gene, the second stage is analyses of genetic diversity of tiger fish within and between population by using the mitochondrial DNA cytochrome b (Cyt b) gene, and nucleus DNA recombination (RAG2) gene. The molecular identification has shown that the collected fish has a genetic similarity of 100% with D. undecimradiatus. The genetic diversity of tiger fish between populations is 0.023 (standard deviation of 0.001) whereas intra-population is 0.002 and 0.003 for Kalimantan and Sumatra, respectively. The genetic distance of samples with species D. undeciumradiatus were 0.003 and 0.006 for Kalimantan and Sumatera, respectively, whereas the genetic distance with D. microlepis was 0.142. The analysis of mutation on RAG2 gene shows there are six nucleotides and three amino acids have mutation.

scholarly journals A PARTIAL SEPARATION AND CHARACTERIZATION OF CYTOCHROME OXIDASE AND CYTOCHROME b

1950 ◽  
Vol 183 (1) ◽  
pp. 89-103 ◽  
Author(s):  
Bertram. Eichel ◽  
W.W. Wainio ◽  
P. Person ◽  
S.J. Cooperstein
Keyword(s):  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Partial Separation

scholarly journals MITOCHONDRIAL BIOGENESIS DURING DIFFERENTIATION OF ARTEMIA SALINA CYSTS

1973 ◽  
Vol 58 (3) ◽  
pp. 643-649 ◽  
Author(s):  
H. Schmitt ◽  
H. Grossfeld ◽  
U. Z. Littauer
Keyword(s):  
Cytochrome C ◽  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Mitochondrial Biogenesis ◽  
Brine Shrimp ◽  
Morphological Changes ◽  
Artemia Salina ◽  
Mitochondrial Morphology ◽  
Second Stage ◽  
Two Stages

Mitochondria isolated from cysts of Artemia salina (brine shrimp) were found to be devoid of cristae and to possess a low respiratory capability. Hydration of the cysts induces marked biochemical and morphological changes in the mitochondria. Their biogenesis proceeds in two stages. The first stage is completed within 1 h and is characterized by a rapid increase in the respiratory capability of the mitochondria, their cytochrome oxidase, cytochrome b, cytochrome c and perhaps some morphological changes. In the second stage there is an increase in the protein-synthesizing capacity of the mitochondria as well as striking changes in mitochondrial morphology leading to the formation of cristae.

scholarly journals Mitochondrial gene expression in small intestinal epithelial cells

1995 ◽  
Vol 308 (2) ◽  
pp. 665-671 ◽  
Author(s):  
T P Mayall ◽  
I Bjarnason ◽  
U Y Khoo ◽  
T J Peters ◽  
A J S Macpherson
Keyword(s):  
Epithelial Cells ◽  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Intestinal Epithelial Cells ◽  
Mitochondrial Gene ◽  
Cytochrome Oxidase I ◽  
Mrna Levels ◽  
Intestinal Epithelial ◽  
Mitochondrial Transcription Factor ◽  
Small Intestinal

Most mitochondrial genes are transcribed as a single large transcript from the heavy strand of mitochondrial DNA, and are subsequently processed into the proximal mitochondrial (mt) 12 S and 16 S rRNAs, and the more distal tRNAs and mRNAs. We have shown that in intestinal epithelial biopsies the steady-state levels of mt 12 S and 16 S rRNA are an order of magnitude greater than those of mt mRNAs. Fractionation of rat small intestinal epithelial cells on the basis of their maturity has shown that the greatest ratios of 12 S mt rRNA/cytochrome b mt mRNA or 12 S mt rRNA/cytochrome oxidase I mt mRNA are found in the surface mature enterocytes, with a progressive decrease towards the crypt immature enteroblasts. Cytochrome b and cytochrome oxidase I mt mRNA levels are relatively uniform along the crypt-villus axis, but fractionation experiments showed increased levels in the crypt base. The levels of human mitochondrial transcription factor A are also greater in immature crypt enteroblasts compared with mature villus enterocytes. These results show that the relative levels of mt rRNA and mRNA are distinctly regulated in intestinal epithelial cells according to the crypt-villus position and differentiation status of the cells, and that there are higher mt mRNA and mt TFA levels in the crypts, consistent with increased transcriptional activity during mitochondrial biogenesis in the immature enteroblasts.

scholarly journals Proton translocation by the mitochondrial cytochrome b-c1 complex is inhibited by NN′-dicyclohexylcarbodi-imide

1982 ◽  
Vol 206 (2) ◽  
pp. 419-421 ◽  
Author(s):  
B D Price ◽  
M D Brand
Keyword(s):  
Electron Transport ◽  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Rat Liver ◽  
Proton Translocation ◽  
Liver Mitochondria ◽  
Rat Liver Mitochondria ◽  
Mitochondrial Cytochrome ◽  
Low Concentrations ◽  
Mitochondrial Cytochrome B

NN'-Dicyclohexylcarbodi-imide at low concentrations decreases the H+/2e ratio for rat liver mitochondria over the span succinate to oxygen from 5.9 +/- 0.3 (mean +/- S.E.M.) to 4.0 +/- 0.1 and for the cytochrome b-c1 complex from 3.8 +/- 0.2 to 1.9 +/- 0.1, but has little effect on the H+/2e ratio of cytochrome oxidase. The decrease in stoicheiometry is due, not to uncoupling or inhibition of electron transport, but to inhibition of proton translocation. NN'-Dicyclohexylcarbodi-imide thus ‘decouples’ proton translocation in the cytochrome b-c1 complex.

scholarly journals Notes on the distribution and habitat of Ochthebius lobicollis Rey, 1885, a poorly known north-western Mediterranean coastal species (Coleoptera: Hydraenidae)

2019 ◽  
Vol 51 (1) ◽  
pp. 51-54
Author(s):  
Ignacio Ribera ◽  
Carles Hernando
Keyword(s):  
Iberian Peninsula ◽  
Cytochrome Oxidase ◽  
New Records ◽  
Genetic Data ◽  
Western Mediterranean ◽  
Cytochrome Oxidase 1 ◽  
Coastal Species ◽  
Gulf Of Lion ◽  
First Time ◽  
North Western

Ochthebius (Ochthebius) lobiccoastal habitatsollis Rey, 1885 is recorded for the first time from the Iberian Peninsula (Girona) and the island of Corsica; new records are also given for the islands of Menorca and Sardinia. The species is known only from coastal habitats through the Gulf of Lion and the Ligurian and Balearic seas, typically living in rockpools of different salinity or small trickles or freshwater runoffs. Genetic data of the cytochrome oxidase 1 gene from Iberian, Menorcan and Sardinian specimens shows less than 1% divergence, suggesting lack of isolation between populations.

Genetic diversity within Scorpio maurus (Scorpiones: Scorpionidae) from morocco: Preliminary evidence based on CO1 mitochondrial DNA sequences

Biologia ◽  
2008 ◽  
Vol 63 (6) ◽  
Author(s):  
Elsa Froufe ◽  
Pedro Sousa ◽  
Paulo Alves ◽  
David Harris
Keyword(s):  
Genetic Diversity ◽  
Mitochondrial Dna ◽  
Cytochrome Oxidase ◽  
Dna Sequences ◽  
Preliminary Analysis ◽  
Mitochondrial Gene ◽  
Sequence Divergence ◽  
Preliminary Evidence ◽  
Evidence Based ◽  
Cytochrome Oxidase 1

AbstractThe large-clawed scorpion, Scorpio maurus, is a medically important scorpion and yet nothing is known regarding genetic diversity within this species. As a preliminary analysis we determined variation within the cytochrome oxidase 1 (CO1) mitochondrial gene from specimens from Morocco. High levels of genetic diversity were found that presented some geographical coherence. Of the two identified subspecies from Morocco, S. maurus birulai and S. maurus fuliginosus, the latter included genetically distinct lineages (8.0% uncorrected sequence divergence), indicating a detailed morphological and molecular revision is needed for this species.

Mammalian dihydroorotate – ubiquinone reductase complex. II. Correlation with cytochrome oxidase, mode of linkage with the cytochrome chain, and general properties

1969 ◽  
Vol 47 (7) ◽  
pp. 725-734 ◽  
Author(s):  
Richard W. Miller ◽  
Jack R. Curry
Keyword(s):  
Cytochrome Oxidase ◽  
Cytochrome B ◽  
Sucrose Gradient ◽  
Gel Filtration ◽  
Temperature Stability ◽  
Mitochondrial Enzymes ◽  
Anaerobic Conditions ◽  
Beef Liver ◽  
And Behavior

Evidence is presented showing that a dihydroorotate-oxidizing system sediments with mitochondrial enzymes of beef liver in a sucrose gradient. Spectrophotometric experiments now indicate that dihydroorotate is as effective as succinate in reducing the cytochrome b content of whole and sonically disrupted mitochondria. The pyrimidine precursor also reduces the entire cytochrome chain under anaerobic conditions. Additional characterization of the dihydroorotate – ubiquinone reductase complex was carried out with regard to pH and temperature stability, and optima, metal inhibition, and behavior in gel filtration chromatography.

Barcoding, phylogeography and species boundaries in clownfishes of the Indian Ocean

DNA Barcodes ◽  
2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Kottila Veettil Dhaneesh ◽  
Thipramalai Thankappan Ajith Kumar ◽  
Appukuttannair Biju Kumar
Keyword(s):  
Indian Ocean ◽  
Cytochrome Oxidase ◽  
Phylogenetic Relationships ◽  
Species Boundaries ◽  
Cytochrome Oxidase 1 ◽  
The Indian Ocean

AbstractIn this study, barcoding of 13 clownfish species of the Indian Ocean was carried out to infer the phylogenetic relationships among them by analyzing cytochrome oxidase 1 (CO1) and cytochrome

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