scholarly journals Retraction: Study on Molecular Qualitative Detection Technology of Potato Scab Disease (IOP Conf. Ser.: Earth Environ. Sci. 769 022010)

2021 ◽  
Vol 769 (2) ◽  
pp. 022076
Keyword(s):  
Fragment Size ◽  
Potato Scab ◽  
Detection Technology ◽  
Qualitative Detection ◽  
Potato Scab Disease ◽  
Different Strains ◽  
Repeated Experiment

This article has been retracted by the authors following correspondence with IOP Publishing in which the authors claim the work is unreliable. The author's explanation follows: "The material of the paper is bacteria, it is not be visible to the naked eyes, the material was contaminated by the drugs that out of date and got polluted, and it caused the serious mistake in the figure 2. The result of different strains of DNA by the primer in 3.3 was not match of our material. The figure 2 was the amplification results of different strains of DNA , and the fragment size of different strains were same in the result, but the result should not be same , so the result was wrong. When we continue to the study, we got new result about the figure 2. There was the amplification results of different strains of DNA by primer TXT in repeated experiment , and the result in the published article was wrong, the material was contaminated. We are afraid that other results were also incorrect in this paper." IOP Publishing cannot verify this information as accurate, however in the interest of transparency and reproducibility, IOP Publishing agrees to retract this article. This notice will be updated if more information comes to light. Retraction published: 21 December 2021

scholarly journals Necrosis Gene-Based Monitoring and Control of Potato Scab Disease

2007 ◽  
Vol 22 (2) ◽  
pp. 123-127 ◽  
Author(s):  
Osamu Koyama ◽  
Akira Manome ◽  
Mayu Okubo ◽  
Toyokazu Yokomaku ◽  
Hideo Tanaka
Keyword(s):  
Potato Scab ◽  
Monitoring And Control ◽  
Potato Scab Disease ◽  
And Control

Differentiation of Streptomyces spp. which cause potato scab disease on the basis of partial rpoB gene sequences

2007 ◽  
Vol 30 (5) ◽  
pp. 401-407 ◽  
Author(s):  
Ho-Suk Mun ◽  
Eun-Ju Oh ◽  
Hyun-Ju Kim ◽  
Keun-Hwa Lee ◽  
Young-Hwan Koh ◽  
...  
Keyword(s):  
Potato Scab ◽  
Rpob Gene ◽  
Gene Sequences ◽  
Streptomyces Spp ◽  
Potato Scab Disease

scholarly journals First Report Regarding Potato Scab Caused by Streptomyces acidiscabies in Uruguay

Plant Disease ◽  
2012 ◽  
Vol 96 (7) ◽  
pp. 1064-1064 ◽  
Author(s):  
M. I. Lapaz ◽  
E. Verdier ◽  
M. J. Pianzzola
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Potato Scab ◽  
Rrna Gene ◽  
Genus Streptomyces ◽  
Pathogenicity Genes ◽  
First Report ◽  
Streptomyces Acidiscabies ◽  
Potato Scab Disease ◽  
The 16S Rrna Gene

Potato scab disease is caused by gram-positive filamentous bacteria in the genus Streptomyces. A great variety of species cause this disease, but Streptomyces scabies is the most ancient of these pathogens and can be found in a worldwide distribution, whereas S. turgidiscabies and S. acidiscabies are newly emerged pathogens (2). During the autumn of 2010, potato (Solanum tuberosum) crops had large economic losses by common scab, corresponding to 29% of the total potato-cultivation area (according to our survey), which was unusual in Uruguay. Specifically, the disease was very aggressive and the tubers showed particularly deep scab lesions. We isolated the Streptomyces species present in these particular scab lesions of tubers collected in July 2010 from one of the three potato cultivation areas (San José). A total of 19 Streptomyces spp. strains were isolated and identified using classical and molecular techniques. Morphological characteristics of colonies and microscopic structure of the mycelium were observed (1). Molecular characterization by conventional PCR was carried out using primers directed to specific regions of the 16S rRNA gene for the genus Streptomyces, Aci1: (5′-TCACTCCTGCCTGCATGGGCG-3′) and Aci2: (5′-CGACAGCTCCCTCCCACAAG-3′). Also, regions of two pathogenicity genes, namely txtAB and nec1, were amplified and confirmed by sequencing (2). Additionally, melanin production and pathogenicity of the isolates was determined by inoculation of potato discs (1). Six of the 19 strains succeeded in PCR amplification with primers specific to Streptomyces acidiscabies, which has white, aerial hypha and flexuous spore chains. These strains did not produce melanin on tyrosine agar media. The amplified fragments for 16S rRNA and pathogenicity genes from one representative strain 61 were sequenced. BLASTn analysis of the 16S rRNA gene sequence obtained of the strain 61 (Accession No. JN206667) showed the highest similarity (100%) with S. acidiscabies type strain 84-01-182 (GenBank Accession No. FJ007427.1). Pathogenicity of the isolate was tested on tuber slices. The isolate was grown on YME for 5 to 7 days at 28°C and agar plugs from the sporulating colonies were inverted onto excised tuber tissue. Disks were incubated at 28°C in the dark and the presence of necrosis was evaluated after 5 days (1). All tuber slice assays were repeated three times. The noninoculated control tuber slices did not show any necrosis, while those inoculated with the strain did. To our knowledge, this is the first report of S. acidiscabies causing potato scab disease in Uruguay. References: (1) D. H. Park et al. Plant Dis. 87:1290, 2003. (2) L. A. Wanner. Phytopathology 96:1361, 2006.

The roles of inoculants’ carbon source use in the biocontrol of potato scab disease

2015 ◽  
Vol 61 (4) ◽  
pp. 257-262 ◽  
Author(s):  
Pingping Sun ◽  
Xinbei Zhao ◽  
Nini Shangguan ◽  
Dongwei Chang ◽  
Qing Ma
Keyword(s):  
Carbon Source ◽  
Plant Disease ◽  
Potato Scab ◽  
Plant Diseases ◽  
Positive Effects ◽  
Potato Scab Disease ◽  
Multiple Strains ◽  
Source Use ◽  
Shed Light

Despite the application of multiple strains in the biocontrol of plant diseases, multistrain inoculation is still constrained by its inconsistency in the field. Nutrients, especially carbons, play an important role in the biocontrol processes. However, little work has been done on the systematic estimation of inoculants’ carbon source use on biocontrol efficacies in vivo. In the present study, 7 nonpathogenic Streptomyces strains alone and in different combinations were inoculated as biocontrol agents against the potato scab disease, under field conditions and greenhouse treatments. The influence of the inoculants’ carbon source use properties on biocontrol efficacies was investigated. The results showed that increasing the number of inoculated strains did not necessarily result in greater biocontrol efficacy in vivo. However, single strains with higher growth rates or multiple strains with less carbon source competition had positive effects on the biocontrol efficacies. These findings may shed light on optimizing the consistent biocontrol of plant disease with the consideration of inoculants’ carbon source use properties.

Ultrastructural Changes of the Symbiotic Chlorella-Like Alga Isolated from Hydra Viridis

1982 ◽  
Vol 40 ◽  
pp. 320-321
Author(s):  
K.W. Lee ◽  
R.H. Meints ◽  
D. Kuczmarski ◽  
J.L. Van Etten
Keyword(s):  
Time Course ◽  
Reciprocal Cross ◽  
Ultrastructural Level ◽  
Ultrastructural Changes ◽  
Symbiotic Relationship ◽  
Cell Recognition ◽  
Green Hydra ◽  
Different Strains ◽  
Hydra Viridis

The physiological, biochemical, and ultrastructural aspects of the symbiotic relationship between the Chlorella-like algae and the hydra have been intensively investigated. Reciprocal cross-transfer of the Chlorellalike algae between different strains of green hydra provide a system for the study of cell recognition. However, our attempts to culture the algae free of the host hydra of the Florida strain, Hydra viridis, have been consistently unsuccessful. We were, therefore, prompted to examine the isolated algae at the ultrastructural level on a time course.

On-line image readout in CTEM

1994 ◽  
Vol 52 ◽  
pp. 400-401
Author(s):  
K.-H. Herrmann ◽  
W. D. Rau ◽  
R. Sikeler
Keyword(s):  
Primary Electron ◽  
Digital Information ◽  
Electron Hole ◽  
Detection Technology ◽  
Long Time ◽  
On Line ◽  
Electron Image ◽  
Optical Transfer ◽  
Detection Quantum Efficiency ◽  
Technical Solutions

Quantitative recording of electron patterns and their rapid conversion into digital information is an outstanding goal which the photoplate fails to solve satisfactorily. For a long time, LLL-TV cameras have been used for EM adjustment but due to their inferior pixel number they were never a real alternative to the photoplate. This situation has changed with the availability of scientific grade slow-scan charged coupled devices (CCD) with pixel numbers exceeding 106, photometric accuracy and, by Peltier cooling, both excellent storage and noise figures previously inaccessible in image detection technology. Again the electron image is converted into a photon image fed to the CCD by some light optical transfer link. Subsequently, some technical solutions are discussed using the detection quantum efficiency (DQE), resolution, pixel number and exposure range as figures of merit.A key quantity is the number of electron-hole pairs released in the CCD sensor by a single primary electron (PE) which can be estimated from the energy deposit ΔE in the scintillator,

Injuries to the Coronoid Process of the Ulna with Involvement of the Lesser Sigmoid Notch

2020 ◽  
Author(s):  
Valentin Rausch ◽  
Sina Neugebauer ◽  
Tim Leschinger ◽  
Lars Müller ◽  
Kilian Wegmann ◽  
...  
Keyword(s):  
Fragment Size ◽  
Coronoid Process ◽  
Annular Ligament ◽  
Concomitant Injuries ◽  
Proximal Ulna ◽  
Sigmoid Notch ◽  
Coronoid Fractures ◽  
The Mean ◽  
The Stability ◽  
Arthritic Changes

Abstract Introduction This study aimed to describe the involvement of the lesser sigmoid notch in fractures to the coronoid process. We hypothesized that injuries to the lateral aspect of the coronoid process regularly involve the annular ligament insertion at the anterior lesser sigmoid notch. Material and Methods Patients treated for a coronoid process fracture at our institution between 06/2011 and 07/2018 were included. We excluded patients < 18 years, patients with arthritic changes or previous operative treatment to the elbow, and patients with concomitant injuries to the proximal ulna. In patients with involvement of the lesser sigmoid notch, the coronoid height and fragment size (anteroposterior, mediolateral, and craniocaudal) were measured. Results Seventy-two patients (mean age: 47 years ± 17.6) could be included in the study. Twenty-one patients (29.2%) had a fracture involving the lateral sigmoid notch. The mean anteroposterior fragment length was 7 ± 1.6 mm. The fragment affected a mean of 43 ± 10.8% of the coronoid height. The mean mediolateral size of the fragment was 10 ± 5.0 mm, and the mean cranio-caudal size was 7 ± 2.7 mm. Conclusion Coronoid fractures regularly include the lesser sigmoid notch. These injuries possibly affect the anterior annular ligament insertion which is important for the stability of the proximal radioulnar joint and varus stability of the elbow.

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