scholarly journals Roles of plant growth regulators on flowering of rose (Rosa hybrida L.’Red Rose’)

2021 ◽  
Vol 947 (1) ◽  
pp. 012039
Author(s):  
Linh Tran Minh Hong ◽  
Tu Cam Trinh ◽  
Viet Trang Bui ◽  
Huong Thanh Tran
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Rosa Hybrida ◽  
Floral Meristem ◽  
Flower Bud ◽  
Meristem Cell

Abstract Rose is the most popular ornamental flower all over the world, which is used as garden plants and cut flowers. In the case of Rosa hybrida L. ’Red Rose’, flowering provides the major developmental transition from the vegetative to the reproductive stage, and reproduction is one of the most important phases in an organism’s life cycle. In this study, the morphological and physiological changes during the flower development of rose, which is planted in the garden, and roles of plant growth regulators on the flowering of in vitro vegetative shoots of rose were analyzed. The development of a flower includes three stages: the shoot apical meristem, floral meristem, floral bud. Levels of cytokinin, auxins, and gibberellins increased in the transition of meristem from the shoot apical meristem to the floral meristem stage. Plant growth regulators have important effects on the shoot apical meristem cell division and flowering. The combination of 0.5 mg.L−1 GA3, 0.1 mg.L−1 NAA, 2.5 or 3.0 mg.L−1 BA to Murashige and Skoog (MS) medium induces the floral transition of the in vitro vegetative shoots with the highest percentage (41%) as well as growth and development in comparison to the other treatments after 10 weeks. Then, the in vitro floral meristem continuously developed into a flower bud after 12 weeks.

scholarly journals Roles of plant growth regulators in the in vitro floral organogenesis of rose (Rosa hybrida L.)

2019 ◽  
Vol 2 (6) ◽  
pp. 98-104
Author(s):  
Tran Minh Hong Linh ◽  
Trinh Cam Tu ◽  
Bui Trang Viet ◽  
Tran Thanh Huong
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Rosa Hybrida ◽  
Floral Meristem ◽  
Floral Organogenesis ◽  
Initiation Stage ◽  
Floral Meristems ◽  
Floral Bud

In this study, morphological and physiological changes in flower development of the rose (Rosa hybrida L.) in the garden were analyzed. Role of plant growth regulators on in vitro floral organogenesis of rose from floral meristem was investigated. The flowering of Rosa hybrida L. has three phases: shoot apical meristem, single floral meristem and floral bud with sepals, petals, stamens and gynoecium. Activities of cytokinins and auxins increased in the transition of shoots from vegetative growth to floral initiation stage. Floral meristems having sepals and the first layer of petals on MS medium with 0.5 mg/L GA3, 0.1 mg/L NAA and 0.3 m/L BA were continuously developed in these next layers of petals and became floral buds at the highest percentage after 4 and 8 weeks of culture, respectively.  

scholarly journals Effect of plant growth regulators and explants on callus induction and study of antioxidant potentials and phenolic metabolites in Salvia tebesana Bunge

2020 ◽  
Vol 44 (2) ◽  
pp. 163-173 ◽  
Author(s):  
Niloofar Hemmati ◽  
Monireh Cheniany ◽  
Ali Ganjeali
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Phenolic Acids ◽  
Growth Regulators ◽  
Callus Induction ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Total Phenolic ◽  
Scale Production

This study was undertaken to investigate the effect of different plant growth regulators (PGRs) on callus induction in Salvia tebesana explants grown in vitro and to evaluate the content of secondary phenolic compounds and their antioxidant potential. The explants (shoot apical meristem, leaf and petiole) were dissected from an 8-week-old plant of S. tebesana growing in vitro and cultured on MS media containing different concentrations of 2,4-D (0, 0.5, 1, 1.5 and 2 mg L-1), NAA (0, 0.5 and 1 mg L-1) and BAP (0, 0.5 and 1 mg L-1), either alone or in a blend with each other. Morphological characteristics of the callus (consistency and colour), biomass increase based on fresh and dry weight and the percentage of induction were recorded after 56 days. Levels of total phenols, ortho-diphenols, phenolic acids, flavonoids, proanthocyanidins and flavonols of callus, as well as antioxidant activities, were evaluated in vitro. The maximum callus formation (100%) was obtained from shoot apical meristem on MS medium supplemented with 0.5 and 1.5 mg L-1 2,4-D + 1 mg L-1 BAP and with 1 and 1.5 mg L-1 2,4-D + 0.5 mg L-1 BAP, whereas the highest fresh (15.06 ? 0.88 g) and dry (0.33 ? 0.02 g) weights of call were observed in a medium containing1.5 mg L-1 2,4-D + 0.5 mg L-1 NAA. It was noted that MS media augmented with combined PGRs had the highest accumulation of polyphenols, phenolic acids and flavonoid compounds, with levels of content varying in the following order: 2,4-D + BAP > NAA + BAP > 2,4-D + NAA. Strong linear correlations were established between total phenolic content of callus extracts and results of the DPPH and FRAP assays (r2 = 0.896 and r2 = 0.946, p < 0.01, respectively). The obtained results suggest that the described method could be utilised as a tool for large-scale production of medicinal metabolites of S. tebesana by tissue culture.

scholarly journals Phytohormones Influence on the In-Vitro Regeneration of Saccharum Officinarum L. from Apical Meristem Culture

2014 ◽  
Vol 5 (2) ◽  
pp. 85 ◽  
Author(s):  
Ejiroghene Felix Lawyer ◽  
Z. O. Jamaleddine ◽  
P. T. Lyam ◽  
I. T. Borokini ◽  
A. A. Adedeji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Apical Meristem ◽  
In Vitro Regeneration ◽  
Saccharum Officinarum ◽  
Naphthalene Acetic Acid ◽  
Root Induction ◽  
Indole Butyric Acid

Growth regulators especially auxins and cytokinins are critical for plant in-vitro regeneration. The effect of these plant growth regulators on in-vitro propagation of Saccharum officinarum L (Sugarcane) was investigated. In vitro response of two different varieties of sugarcane (NCS 005 and NCS 008) to Plant Growth Regulators was obtained in this study. Formation of buds was obtained on shoot apical meristem when cultured on MS (Murashige and Skoog) medium supplemented with 0.1mg/l BAP (6-Benzylaminopurine). After two weeks of initiation, regenerated meristem was inoculated into MS (Murashige and Skoog) fortified with different concentrations and combination of cytokinins. Shoot multiplication was optimal on 0.5mg/l BAP + 0.25 mg/l Kin(Kinetin) for NCS 005 variety while for NCS 008 variety, no significant (P≥0.05) difference was observed between 1.5mg/l BAP and 1.5mg/l BAP +0.5mg/l Kin. The best root induction for in vitro derived shoots was obtained on 1.0 mg/l NAA (Naphthalene acetic acid) and 2.0 mg/l IBA( Indole butyric acid) for both varieties of sugarcane within ten days of culture transfer. Successfully established plantlets showed excellent growth response when weaned under regulated green house conditions.

scholarly journals Effects of Plant Growth Regulators on in vitro Propagation of Economically Important Ornamental Plant Rosa hybrida L.

2020 ◽  
Vol 9 (2) ◽  
pp. 227-233
Author(s):  
Diwakar Aggarwal ◽  
Sushil Kumar Upadhyay ◽  
Kuldeep Kumar ◽  
Nirmala Sehrawat ◽  
Hardeep Singh Tuli ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
In Vitro Propagation ◽  
Ornamental Plant ◽  
Rosa Hybrida

scholarly journals STUDY ON FACTORS AFFECTING THE FLOWERING OF FORGET-ME-NOT (Browallia americana L.) IN VITRO CULTURE

2020 ◽  
Vol 1 (40) ◽  
pp. 13-27
Author(s):  
Linh Thi Thuy Le ◽  
Thuong Tieu Linh Tran ◽  
Hung Duc Le ◽  
Dien Huynh Han ◽  
Thy Thi Bich Le ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Great Influence ◽  
Basic Research ◽  
Flower Buds ◽  
Flower Bud ◽  
Factors Affecting ◽  
Flowering Rate

Vegetative to reproductive transition depends on different factors. This study was conducted to examine factors affecting the growth and flowering of Browallia americana L. in vitro such as the age of the sample, mineral content, plant growth regulators, concentration and type of sugar, ventilation culture. The results showed that 40-day-old shoots were a suitable source of in vitro flowering (90.85% after 45 days of culture). Mineralcontent had a great influence on the ability to differentiate flower buds of plants, the highest in Murashige and Skoog medium (87.22% after 45 days of culture). Meanwhile, most of the plant growth regulators which were surveyed in this study inhibited the flowering. In particular, culture medium with glucose showed higher flowering efficiency than saccharose, the highest flowering rate was at 50 mg/L glucoses (90.52%, with 3.50 flower buds/explant after 30 days of culture). In addition, ventilation culture, which used plastic wrapedwith milipore filter had the highest rate of flower bud formation (average 3.58 flower buds/plant). This result is an important foundation for studying the flowering of in vitro plant culture, which will be one of the great paradigm plants for teaching and basic research.

scholarly journals Rolesof plant growth regulators on the shoot development from the shoot apical meristem of Eustoma grandiflorum (RAF.) shinners

2018 ◽  
Vol 1 (6) ◽  
pp. 58-67
Author(s):  
Huyen Thach Quynh Ngo ◽  
Huong Thanh Tran ◽  
Viet Trang Bui
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Shoot Apical Meristem ◽  
Apical Meristem ◽  
Adventitious Shoots ◽  
Shoot Development ◽  
Ms Medium ◽  
Eustoma Grandiflorum ◽  
Shoot Initiation

In this paper, plant growth regulators including 6-benzylaminopurine (BA), kinetin, indole-3-acetic acid (IAA), gibberellic acid (GA3) and ethrel, at different concentrations were used individually or in combination to induce adventitious shoots from the explants, which contain shoot apical meristem and young leaves. Histological and physiological changes during shoot development were analysed. The highest shoot initiation was achieved on Murashige and Skoog (MS) medium supplemented with 0.5 mg/L BA and 1.0 mg/L GA3. Regenerated shoots were rooted on MS medium with 0.25 or 0.5 mg/L IAA. Shoot development from in vitro shoot explants initiated from the axil and cortex of stem. The shoot regeneration from shoot apical explants was effected by the meristem integrity or auxin from shoot apical meristem. Roles of plant growth regulators, especially polar auxin transport, and the ablation on the shoot initiation were discussed.

scholarly journals Alterations in Microrhizome Induction, Shoot Multiplication and Rooting of Ginger (Zingiber officinale Roscoe) var. Bentong with Regards to Sucrose and Plant Growth Regulators Application

Agronomy ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 320
Author(s):  
Nisar Ahmad Zahid ◽  
Hawa Z.E. Jaafar ◽  
Mansor Hakiman
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Zingiber Officinale ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Zingiber Officinale Roscoe ◽  
Planting Materials ◽  
Disease Free

Ginger (Zingiber officinale Roscoe) var. Bentong is a monocotyledon plant that belongs to the Zingiberaceae family. Bentong ginger is the most popular cultivar of ginger in Malaysia, which is conventionally propagated by its rhizome. As its rhizomes are the economic part of the plant, the allocation of a large amount of rhizomes as planting materials increases agricultural input cost. Simultaneously, the rhizomes’ availability as planting materials is restricted due to the high demand for fresh rhizomes in the market. Moreover, ginger propagation using its rhizome is accompanied by several types of soil-borne diseases. Plant tissue culture techniques have been applied to produce disease-free planting materials of ginger to overcome these problems. Hence, the in vitro-induced microrhizomes are considered as alternative disease-free planting materials for ginger cultivation. On the other hand, Bentong ginger has not been studied for its microrhizome induction. Therefore, this study was conducted to optimize sucrose and plant growth regulators (PGRs) for its microrhizome induction. Microrhizomes were successfully induced in Murashige and Skoog (MS) medium supplemented with a high sucrose concentration (>45 g L−1). In addition, zeatin at 5–10 µM was found more effective for microrhizome induction than 6-benzylaminopurine (BAP) at a similar concentration. The addition of 7.5 µM 1-naphthaleneacetic acid (NAA) further enhanced microrhizome formation and reduced sucrose’s required dose that needs to be supplied for efficient microrhizome formation. MS medium supplemented with 60 g L−1 sucrose, 10 µM zeatin and 7.5 µM NAA was the optimum combination for the microrhizome induction of Bentong ginger. The in vitro-induced microrhizomes sprouted indoors in moist sand and all the sprouted microrhizomes were successfully established in field conditions. In conclusion, in vitro microrhizomes can be used as disease-free planting materials for the commercial cultivation of Bentong ginger.

Sign in / Sign up

Export Citation Format

Share Document