Oil palm frond juice and coconut water as alternative fermentation substrate for bacterial cellulose production

Bacterial Cellulose (BC) was produced by Acetobacterxylinum TISTR 086 use mixing of oil palm shoot juices (OPSJ) and coconut juice (CJ) medium with or without nutrient supplemented or OPSJ was digested with α-amylase. Results showed the highest yield was obtained at OPSJ with CJ at mixing ratios of 50% (4.70±0.11 g/L) and the lowest yield was obtained at OPSJ with CJ at mixing ratios of 0% (3.54±0.11 g/L). OPSJ was digested with 1300 lU/gm at concentration enzyme 650 IU/L at 37 °C for 24 h has highest sugar concentration of 73.93±0.12 g/L. Amylase digested OPSJ mixed with coconut juice’s highest bacterial cellulose production at OPSJ with CJ at mixing ratios of 50% (4.94±0.13 g/L), respectively. Moreover, the BC obtained had molecular weight from 332,122 to 503,233 g/mol. The chemical structure of BC produced was studied using Fourier Transform Infrared Spectroscopy (FT-IR) and X-ray defections. The morphology of BC was investigated by Scanning Electron Microscopy (SEM). Thermal property was further studied using Differential Scanning Calorimetry (DSC) and Thermogravimetic Analysis (TGA). This study obviously showed the ability of OPSJ, a suitable and cheap carbon source, to be used as a substrate in a fermentation medium for production of cellulose by Acetobacterxylinum TISTR 086.

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Influence of drying method on the crystal structure and thermal property of oil palm frond juice-based bacterial cellulose

Journal of Materials Science ◽

10.1007/s10853-021-06685-5 ◽

2022 ◽

Author(s):

Shahril Mohamad ◽

Luqman Chuah Abdullah ◽

Saidatul Shima Jamari ◽

Syeed Saifulazry Osman Al Edrus ◽

Min Min Aung ◽

...

Keyword(s):

Crystal Structure ◽

Thermal Property ◽

Bacterial Cellulose ◽

Oil Palm ◽

Drying Method ◽

Oil Palm Frond ◽

Palm Frond

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Oil Palm Frond Juice as Future Fermentation Substrate: A Feasibility Study

BioMed Research International ◽

10.1155/2014/465270 ◽

2014 ◽

Vol 2014 ◽

pp. 1-8 ◽

Cited By ~ 5

Author(s):

Che Mohd Hakiman Che Maail ◽

Hidayah Ariffin ◽

Mohd Ali Hassan ◽

Umi Kalsom Md Shah ◽

Yoshihito Shirai

Keyword(s):

Oil Palm ◽

Microbial Growth ◽

Storage Stability ◽

Product Formation ◽

Storage Duration ◽

Industrial Fermentation ◽

Oil Palm Frond ◽

And Storage ◽

Palm Frond ◽

Fermentation Substrate

Oil palm frond (OPF) juice is a potential industrial fermentation substrate as it has high sugars content and the OPF are readily available daily. However, maximum sugars yield and storage stability of the OPF juice are yet to be determined. This study was conducted to determine the effect of physical pretreatment and storage duration of OPF petiole on sugars yield. Storage stability of OPF juice at different storing conditions was also investigated. It was found that OPF petiole squeezed by hydraulic pressing machine gave the highest sugars recovery at almost 40 g/kg, accounting for a recovery yield of 88%. Storage of OPF petiole up to 72 hrs prior to squeezing reduced the free sugars by 11 g/kg. Concentrated OPF juice with 95% water removal had the best storage stability at both 4 and30°C, when it was stored for 10 days. Moreover, concentrated OPF syrup prepared by thermal processing did not give any Maillard effect on microbial growth. Based on our results, OPF juice meets all the criteria as a good fermentation substrate as it is renewable, consistently available, and easy to be obtained, it does not inhibit microbial growth and product formation, and it contains no impurities.

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