Repairing a bone defect with a three-dimensional cellular construct composed of a multi-layered cell sheet on electrospun mesh

AbstractMethods to repair bone defects arising from trauma, resection, or disease, continue to be sought after. Cyclic mechanical loading is well established to influence bone (re)modelling activity, in which bone formation and resorption are correlated to micro-scale strain. Based on this, the application of mechanical stimulation across a bone defect could improve healing. However, if ignoring the mechanical integrity of defected bone, loading regimes have a high potential to either cause damage or be ineffective. This study explores real-time finite element (rtFE) methods that use three-dimensional structural analyses from micro-computed tomography images to estimate effective peak cyclic loads in a subject-specific and time-dependent manner. It demonstrates the concept in a cyclically loaded mouse caudal vertebral bone defect model. Using rtFE analysis combined with adaptive mechanical loading, mouse bone healing was significantly improved over non-loaded controls, with no incidence of vertebral fractures. Such rtFE-driven adaptive loading regimes demonstrated here could be relevant to clinical bone defect healing scenarios, where mechanical loading can become patient-specific and more efficacious. This is achieved by accounting for initial bone defect conditions and spatio-temporal healing, both being factors that are always unique to the patient.

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Effects of Velvet Antler Polypeptide-Collagen/Chitosan Materials on Proliferation of Osteoblast

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.380-384.4295 ◽

2013 ◽

Vol 380-384 ◽

pp. 4295-4298

Author(s):

Wen He Zhu ◽

Jun Jie Xu ◽

Wei Zhang ◽

Yan Li ◽

Xiao Jing Lu ◽

...

Keyword(s):

Drug Treatment ◽

Bone Defect ◽

Three Dimensional ◽

Chitosan Scaffold ◽

Release Drug ◽

Dimensional Network ◽

Velvet Antler ◽

Result Show ◽

Long Time ◽

Velvet Antler Polypeptide

A highly osteogenic hybrid bioabsorbable scaffold was developed for bone reconstruction. Though the use of a bioabsorbable collagen and chitosan scaffold for loading velvet antler polypeptide to repair bone defect and drug treatment. Velvet antler polypeptide and collagen were extracted for developing the compounded material. The SEM results show that the collagen and chitosan scaffold maintain the natural three dimensional network structures. The cell proliferation experiment result show that the can promote the osteoblast proliferation for a long time . These results indicated that this compound scaffold can sustainable to release drug and is a good material in bone defect and drug treatment.

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Functional Analysis of Induced Human Ballooned Hepatocytes in a Cell Sheet-Based Three Dimensional Model

Tissue Engineering and Regenerative Medicine ◽

10.1007/s13770-020-00297-x ◽

2021 ◽

Author(s):

Botao Gao ◽

Katsuhisa Sakaguchi ◽

Tetsuya Ogawa ◽

Yuki Kagawa ◽

Hirotsugu Kubo ◽

...

Keyword(s):

Functional Analysis ◽

Cell Sheet ◽

Three Dimensional ◽

Dimensional Model ◽

Three Dimensional Model ◽

A Cell

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Three-dimensional-printed individualized porous implants: A new “implant-bone” interface fusion concept for large bone defect treatment

Bioactive Materials ◽

10.1016/j.bioactmat.2021.03.030 ◽

2021 ◽

Vol 6 (11) ◽

pp. 3659-3670

Author(s):

Teng Zhang ◽

Qingguang Wei ◽

Hua Zhou ◽

Zehao Jing ◽

Xiaoguang Liu ◽

...

Keyword(s):

Bone Defect ◽

Three Dimensional ◽

Large Bone Defect ◽

Bone Interface ◽

Fusion Concept ◽

Bone Defect Treatment ◽

Large Bone

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Rapid fabrication system for three-dimensional tissues using cell sheet engineering and centrifugation

Journal of Biomedical Materials Research Part A ◽

10.1002/jbm.a.35526 ◽

2015 ◽

Vol 103 (12) ◽

pp. 3825-3833 ◽

Cited By ~ 8

Author(s):

Akiyuki Hasegawa ◽

Yuji Haraguchi ◽

Tatsuya Shimizu ◽

Teruo Okano

Keyword(s):

Cell Sheet ◽

Three Dimensional ◽

Cell Sheet Engineering ◽

Rapid Fabrication

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Abstract 020: Cell-sheet-based Bioengineered Human Cardiac Tissue Using Pluripotent Stem Cells For Heart Repair And Disease Models

Circulation Research ◽

10.1161/res.113.suppl_1.a020 ◽

2013 ◽

Vol 113 (suppl_1) ◽

Author(s):

Katsuhisa Matsuura ◽

Tatsuya Shimizu ◽

Nobuhisa Hagiwara ◽

Teruo Okano

Keyword(s):

Cardiac Tissue ◽

Subcutaneous Tissue ◽

Cell Sheet ◽

Three Dimensional ◽

Embryoid Bodies ◽

Cardiac Differentiation ◽

Cardiac Cell ◽

Cell Sheets ◽

High Efficient ◽

Human Cardiac Tissue

We have developed an original scaffold-free tissue engineering approach, “cell sheet engineering”, and this technology has been already applied to regenerative medicine of various organs including heart. As the bioengineered three-dimensional cardiac tissue is expected to not only function for repairing the broad injured heart but also to be the practicable heart tissue models, we have developed the cell sheet-based perfusable bioengineered three-dimensional cardiac tissue. Recently we have also developed the unique suspension cultivation system for the high-efficient cardiac differentiation of human iPS cells. Fourteen-day culture with the serial treatments of suitable growth factors and a small compound in this stirring system with the suitable dissolved oxygen concentration produced robust embryoid bodies that showed the spontaneous beating and were mainly composed of cardiomyocytes (~80%). When these differentiated cells were cultured on temperature-responsive culture dishes after the enzymatic dissociation, the spontaneous and synchronous beating was observed accompanied with the intracellular calcium influx all over the area even after cell were detached from culture dishes as cell sheets by lowering the culture temperature. The cardiac cell sheets were mainly composed of cardiomyocytes (~80%) and partially mural cells (~20%). Furthermore, extracellular action potential propagation was observed between cell sheets when two cardiac cell sheets were partially overlaid, and this propagation was inhibited by the treatment with some anti-arrhythmic drugs. When the triple layered cardiac tissue was transplanted onto the subcutaneous tissue of nude rats, the spontaneous pulsation was observed over 2 months and engrafted cardiomyocytes were vascularized with the host tissue-derived endothelial cells. These findings suggest that cardiac cell sheets formed by hiPSC-derived cardiomyocytes might have sufficient properties for the creation of thickened cardiac tissue. Now we are developing the vascularized thickened human cardiac tissue by the repeated layering of cardiac cell sheets on the artificial vascular bed in vitro.

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Evaluation of cell sheet application on one wall bone defect in Macaca nemestrina through periostin expression

Journal of Physics Conference Series ◽

10.1088/1742-6596/884/1/012039 ◽

2017 ◽

Vol 884 ◽

pp. 012039

Author(s):

R Y Tamin ◽

Y Soeroso ◽

L Amir ◽

E Idrus

Keyword(s):

Bone Defect ◽

Cell Sheet ◽

Macaca Nemestrina

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In Vivo Reconstruction of the Acetabular Bone Defect by the Individualized Three-Dimensional Printed Porous Augment in a Swine Model

BioMed Research International ◽

10.1155/2020/4542302 ◽

2020 ◽

Vol 2020 ◽

pp. 1-10

Author(s):

Jun Fu ◽

Yi Xiang ◽

Ming Ni ◽

Xiaojuan Qu ◽

Yonggang Zhou ◽

...

Keyword(s):

Compressive Strength ◽

Elastic Modulus ◽

Bone Defect ◽

Bone Ingrowth ◽

Structural Parameters ◽

Three Dimensional ◽

Acetabular Bone ◽

Acetabular Bone Defect ◽

3D Printed ◽

Matching Degree

Background and Purpose. This study established an animal model of the acetabular bone defect in swine and evaluated the bone ingrowth, biomechanics, and matching degree of the individualized three-dimensional (3D) printed porous augment. Methods. As an acetabular bone defect model created in Bama miniswine, an augment individually fabricated by 3D print technique with Ti6Al4V powders was implanted to repair the defect. Nine swine were divided into three groups, including the immediate biomechanics group, 12-week biomechanics group, and 12-week histological group. The inner structural parameters of the 3D printed porous augment were measured by scanning electron microscopy (SEM), including porosity, pore size, and trabecular diameter. The matching degree between the postoperative augment and the designed augment was assessed by CT scanning and 3D reconstruction. In addition, biomechanical properties, such as stiffness, compressive strength, and the elastic modulus of the 3D printed porous augment, were measured by means of a mechanical testing machine. Moreover, bone ingrowth and implant osseointegration were histomorphometrically assessed. Results. In terms of the inner structural parameters of the 3D printed porous augment, the porosity was 55.48 ± 0.61 % , pore size 319.23 ± 25.05 μ m , and trabecular diameter 240.10 ± 23.50 μ m . Biomechanically, the stiffness was 21464.60 ± 1091.69 N / mm , compressive strength 231.10 ± 11.77 MPa , and elastic modulus 5.35 ± 0.23 GPa , respectively. Furthermore, the matching extent between the postoperative augment and the designed one was up to 91.40 ± 2.83 % . Besides, the maximal shear strength of the 3D printed augment was 929.46 ± 295.99 N immediately after implantation, whereas the strength was 1521.93 ± 98.38 N 12 weeks after surgery ( p = 0.0302 ). The bone mineral apposition rate (μm per day) 12 weeks post operation was 3.77 ± 0.93 μ m / d . The percentage bone volume of new bone was 22.30 ± 4.51 % 12 weeks after surgery. Conclusion. The 3D printed porous Ti6Al4V augment designed in this study was well biocompatible with bone tissue, possessed proper biomechanical features, and was anatomically well matched with the defect bone. Therefore, the 3D printed porous Ti6Al4V augment possesses great potential as an alternative for individualized treatment of severe acetabular bone defects.

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