Isolation and Characterization of Four Developmentally Regulated Cathepsin B-Like Cysteine Protease Genes from the Nematode Caenorhabditis elegans

1996 ◽  
Vol 15 (1) ◽  
pp. 75-82 ◽  
Author(s):  
CHRISTOPHER G.C. LARMINIE ◽  
IAIN L. JOHNSTONE
Keyword(s):  
Caenorhabditis Elegans ◽  
Cysteine Protease ◽  
Cathepsin B ◽  
Nematode Caenorhabditis Elegans ◽  
Developmentally Regulated ◽  
Isolation And Characterization

Isolation and characterization of a sperm-specific gene family in the nematode Caenorhabditis elegans

1984 ◽  
Vol 4 (3) ◽  
pp. 529-537
Author(s):  
M R Klass ◽  
S Kinsley ◽  
L C Lopez
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Specific Gene ◽  
Cdna Clones ◽  
Major Sperm Protein ◽  
Adult Males ◽  
Flanking Sequence ◽  
Nematode Caenorhabditis Elegans ◽  
Developmentally Regulated ◽  
Isolation And Characterization

The major sperm protein (MSP) of the nematode Caenorhabditis elegans is a low-molecular-weight (15,000) basic protein implicated in the pseudopodial movement of mature spermatozoa. Its synthesis occurs in a specific region of the gonad and is regulated at the level of transcription (M. Klass and D. Hirsh, Dev. Biol. 84:299-312, 1981; S. Ward and M. Klass, Dev. Biol. 92:203-208, 1982; Klass et al., Dev. Biol. 93:152-164, 1982). A developmentally regulated gene family has been identified that codes for this MSP. Whole genomic blots, as well as analysis of genomic clone banks, indicate that there are between 15 and 25 copies of the MSP gene in the nematode genome. Southern blot analysis also indicates that there is no rearrangement or amplification within the MSP gene family during development. No evidence was found of methylation at various restriction sites surrounding the MSP gene family, and similarly, no correlation between methylation and expression was observed. Three distinct members of this MSP gene family have been cloned, and their nucleotide sequences have been determined. Differential screening of a cDNA clone bank made from polyadenylated mRNA from adult males yielded 45 male-specific clones, 32 of which were clones of MSP genes. One of these cDNA clones was found to contain the entire nucleotide sequence for the MSP, including part of the 5' leader and all of the 3' trailing sequence. Genomic clones bearing copies of the MSP genes have been isolated. At least one of the members of this gene family is a pseudogene. Another member of the MSP gene family that has been cloned from genomic DNA contains the entire uninterrupted structural sequence for the MSP in addition to a 5' flanking sequence containing a promoter-like region with the classic TATA box, a sequence resembling the CAAT box, and a putative ribosome-binding sequence. The 3' trailing sequences of the genomic and the cDNA clones contain an AATAAA polyadenylation site.

scholarly journals Isolation and characterization of a sperm-specific gene family in the nematode Caenorhabditis elegans.

1984 ◽  
Vol 4 (3) ◽  
pp. 529-537 ◽  
Author(s):  
M R Klass ◽  
S Kinsley ◽  
L C Lopez
Keyword(s):  
Caenorhabditis Elegans ◽  
Gene Family ◽  
Specific Gene ◽  
Cdna Clones ◽  
Major Sperm Protein ◽  
Adult Males ◽  
Flanking Sequence ◽  
Nematode Caenorhabditis Elegans ◽  
Developmentally Regulated ◽  
Isolation And Characterization

The major sperm protein (MSP) of the nematode Caenorhabditis elegans is a low-molecular-weight (15,000) basic protein implicated in the pseudopodial movement of mature spermatozoa. Its synthesis occurs in a specific region of the gonad and is regulated at the level of transcription (M. Klass and D. Hirsh, Dev. Biol. 84:299-312, 1981; S. Ward and M. Klass, Dev. Biol. 92:203-208, 1982; Klass et al., Dev. Biol. 93:152-164, 1982). A developmentally regulated gene family has been identified that codes for this MSP. Whole genomic blots, as well as analysis of genomic clone banks, indicate that there are between 15 and 25 copies of the MSP gene in the nematode genome. Southern blot analysis also indicates that there is no rearrangement or amplification within the MSP gene family during development. No evidence was found of methylation at various restriction sites surrounding the MSP gene family, and similarly, no correlation between methylation and expression was observed. Three distinct members of this MSP gene family have been cloned, and their nucleotide sequences have been determined. Differential screening of a cDNA clone bank made from polyadenylated mRNA from adult males yielded 45 male-specific clones, 32 of which were clones of MSP genes. One of these cDNA clones was found to contain the entire nucleotide sequence for the MSP, including part of the 5' leader and all of the 3' trailing sequence. Genomic clones bearing copies of the MSP genes have been isolated. At least one of the members of this gene family is a pseudogene. Another member of the MSP gene family that has been cloned from genomic DNA contains the entire uninterrupted structural sequence for the MSP in addition to a 5' flanking sequence containing a promoter-like region with the classic TATA box, a sequence resembling the CAAT box, and a putative ribosome-binding sequence. The 3' trailing sequences of the genomic and the cDNA clones contain an AATAAA polyadenylation site.

scholarly journals fog-2, a germ-line-specific sex determination gene required for hermaphrodite spermatogenesis in Caenorhabditis elegans.

Genetics ◽  
1988 ◽  
Vol 119 (1) ◽  
pp. 43-61 ◽  
Author(s):  
T Schedl ◽  
J Kimble
Keyword(s):  
Caenorhabditis Elegans ◽  
Sex Determination ◽  
Germ Line ◽  
Negative Regulator ◽  
Loss Of Function ◽  
Wild Type ◽  
Negative Regulators ◽  
Nematode Caenorhabditis Elegans ◽  
Isolation And Characterization

Abstract This paper describes the isolation and characterization of 16 mutations in the germ-line sex determination gene fog-2 (fog for feminization of the germ line). In the nematode Caenorhabditis elegans there are normally two sexes, self-fertilizing hermaphrodites (XX) and males (XO). Wild-type XX animals are hermaphrodite in the germ line (spermatogenesis followed by oogenesis), and female in the soma. fog-2 loss-of-function mutations transform XX animals into females while XO animals are unaffected. Thus, wild-type fog-2 is necessary for spermatogenesis in hermaphrodites but not males. The fem genes and fog-1 are each essential for specification of spermatogenesis in both XX and XO animals. fog-2 acts as a positive regulator of the fem genes and fog-1. The tra-2 and tra-3 genes act as negative regulators of the fem genes and fog-1 to allow oogenesis. Two models are discussed for how fog-2 might positively regulate the fem genes and fog-1 to permit spermatogenesis; fog-2 may act as a negative regulator of tra-2 and tra-3, or fog-2 may act positively on the fem genes and fog-1 rendering them insensitive to the negative action of tra-2 and tra-3.

Isolation and Characterization of a Rice Cysteine Protease Gene, OsCP1, Using T-DNA Gene-Trap System

2004 ◽  
Vol 54 (5) ◽  
pp. 755-765 ◽  
Author(s):  
Sanghyun Lee ◽  
Ki-Hong Jung ◽  
Gynheung An ◽  
Yong-Yoon Chung
Keyword(s):  
Cysteine Protease ◽  
Gene Trap ◽  
Protease Gene ◽  
Isolation And Characterization ◽  
Trap System ◽  
Cysteine Protease Gene

Fragile skeletal muscle attachments in dystrophic mutants of Caenorhabditis elegans: isolation and characterization of the mua genes

Development ◽  
2000 ◽  
Vol 127 (6) ◽  
pp. 1197-1207 ◽  
Author(s):  
J.D. Plenefisch ◽  
X. Zhu ◽  
E.M. Hedgecock
Keyword(s):  
Caenorhabditis Elegans ◽  
Body Wall ◽  
Local Strain ◽  
Muscle Differentiation ◽  
The Body ◽  
Tissue Separation ◽  
New Genes ◽  
Isolation And Characterization ◽  
Skin Fragility

Over 30 Caenorhabditis elegans mutants were identified with normal muscle differentiation and initial locomotion followed by catastrophic detachment of skeletal muscles from the body wall. Reducing the strength of muscle contraction in these mutants with a myosin gene mutation suppresses muscle detachment. These dystrophic mutants identify a novel class of genes required for growth and maintenance of functional muscle attachments, not exceptional alleles of genes required for muscle differentiation and contractility. Nine new genes, named mua, and two previously published loci, unc-23 and vab-10, cause fragile musscle attachments. The primary sites of muscle detachment, including the plane of tissue separation, are characteristic for each gene. We suggest these genes identify feedback mechanisms whereby local strain regulates the extent of myofibril contraction and the placement of new muscle attachments in functioning muscles. Finally, we draw some comparisons to vertebrate skin fragility diseases and muscular dystrophies.

Isolation and characterization of cathepsin B from bovine brain

1986 ◽  
Vol 11 (6) ◽  
pp. 851-867 ◽  
Author(s):  
John D. Bradley ◽  
John N. Whitaker
Keyword(s):  
Cathepsin B ◽  
Bovine Brain ◽  
Isolation And Characterization
Sign in / Sign up

Export Citation Format

Share Document