Specific Detection of Dog Podoplanin Expressed in Renal Glomerulus by a Novel Monoclonal Antibody PMab-38 in Immunohistochemistry

2016 ◽  
Vol 35 (4) ◽  
pp. 212-216 ◽  
Author(s):  
Ryusuke Honma ◽  
Mika K. Kaneko ◽  
Satoshi Ogasawara ◽  
Yuki Fujii ◽  
Satoru Konnai ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Renal Glomerulus ◽  
Specific Detection

Specific detection of Haemophilus influenzae type b lipooligosaccharide by a polymyxin B monoclonal antibody assay

1989 ◽  
Vol 122 (2) ◽  
pp. 219-226 ◽  
Author(s):  
Jussi Mertsola ◽  
Octavio Ramilo ◽  
Xavier Sáez-Llorens ◽  
Mark S. Hanson ◽  
George H. McCracken ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Haemophilus Influenzae ◽  
Polymyxin B ◽  
Haemophilus Influenzae Type ◽  
Haemophilus Influenzae Type B ◽  
Type B ◽  
Specific Detection ◽  
Antibody Assay

scholarly journals Elisa preliminary studies of immobilization and specific detection of bacterial strains

2020 ◽  
Vol 2 (2) ◽  
pp. 64-69
Author(s):  
Madalina Mihalache ◽  
◽  
Alina Banciu ◽  
Lucian Ionescu ◽  
Mihai Nita-Lazar
Keyword(s):  
Monoclonal Antibody ◽  
Fluorescence Intensity ◽  
Polyclonal Antibody ◽  
Specific Binding ◽  
Negative Control ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bacterial Strains ◽  
Specific Detection ◽  
E Coli ◽  
Alexa Fluor

The paper aims to emphasize the specific detection of bacterial strains using enzyme-linked immunosorbent assay. The assay is based on the specific binding of polyclonal antibody anti-E. coli tagged with FITC to E.coli and monoclonal antibody anti-Ps. aeruginosa tagged with Alexa Fluor 647 tagged to Ps. aeruginosa and on subsequent enzymatic immunological demonstration of the conjugated enzyme. In this experiment, the negative control was the Salmonella enterica strain. The two antibodies had no interaction with the negative control, instead, they were specific for E. coli and Ps. aeruginosa strains. When both strains were in the same well, the fluorescence intensity given by the presence of E. coli was 2.3 times higher than that given by Ps. aeruginosa, and the intensity of fluorescence decreased if there are both bacterial strains in the wells.

scholarly journals Biosensor Based on Cysteine Monolayer and Monoclonal Antibody for Specific Detection of Aflatoxin B1in Rice

2015 ◽  
Author(s):  
Estéfani P. Simão ◽  
Gilcelia J. L. S. Barbieri ◽  
Cesar A. S. Andrade ◽  
Maria D. L. Oliveira
Keyword(s):  
Monoclonal Antibody ◽  
Specific Detection

scholarly journals Monoclonal antibody based immunodot for specific detection of proteins of the shrimp Penaeus species

2011 ◽  
Vol 51 (5) ◽  
pp. 964-969
Author(s):  
P. B. Abhiman ◽  
K. M. Shankar ◽  
Rajreddy Patil ◽  
P. P. Suresh Babu ◽  
A. K. Sahoo ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Specific Detection

scholarly journals Antigen Capture Enzyme-Linked Immunosorbent Assay for Specific Detection of Reston Ebola Virus Nucleoprotein

2003 ◽  
Vol 10 (4) ◽  
pp. 552-557 ◽  
Author(s):  
Tetsuro Ikegami ◽  
Masahiro Niikura ◽  
Masayuki Saijo ◽  
Mary E. Miranda ◽  
Alan B. Calaor ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Amino Acid ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Ebola Virus ◽  
Enzyme Linked Immunosorbent Assay ◽  
Amino Acid Residues ◽  
Specific Detection ◽  
Antigen Capture ◽  
Cynomolgus Macaques

ABSTRACT Antigen capture enzyme-linked immunosorbent assay (ELISA) is one of the most useful methods to detect Ebola virus rapidly. We previously developed an antigen capture ELISA using a monoclonal antibody (MAb), 3-3D, which reacted not only to the nucleoprotein (NP) of Zaire Ebola virus (EBO-Z) but also to the NPs of Sudan (EBO-S) and Reston Ebola (EBO-R) viruses. In this study, we developed antigen capture ELISAs using two novel MAbs, Res2-6C8 and Res2-1D8, specific to the NP of EBO-R. Res2-6C8 and Res2-1D8 recognized epitopes consisting of 4 and 8 amino acid residues, respectively, near the C-terminal region of the EBO-R NP. The antigen capture ELISAs using these two MAbs detected the EBO-R NP in the tissues from EBO-R-infected cynomolgus macaques. The antigen capture ELISAs using Res2-6C8 and Res2-1D8 are useful for the rapid detection of the NP in EBO-R-infected cynomolgus macaques.

scholarly journals Immunohistochemical and Pathological Study of Mycoplasma Bovis-Associated Lung Abscesses in Calves

1995 ◽  
Vol 7 (3) ◽  
pp. 333-337 ◽  
Author(s):  
David S. Adegboye ◽  
Patrick G. Halbur ◽  
David L. Cavanaugh ◽  
Ronald E. Werdin ◽  
Christopher C. L. Chase ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Close Association ◽  
Immunoperoxidase Staining ◽  
Mycoplasma Bovis ◽  
Immunoperoxidase Technique ◽  
Mononuclear Cell Infiltration ◽  
Specific Detection ◽  
Epithelial Surface ◽  
Chronic Pneumonia ◽  
Culture Positive

Out of 45 cases of fatal chronic pneumonia in calves examined for Mycoplasma bovis infection from February to July 1994, 11 cases with pulmonary abscesses that were culture positive for M. bovis were encountered. The cases were studied in detail using a recently developed monoclonal antibody-based immunoperoxidase technique. Mycoplasma bovis organisms were detected in specific locations at all stages of abscessation observed. In bronchioles or terminal airways within which abscesses developed, M. bovis was located at the epithelial surface and in close association with infiltrating neutrophils and macrophages. Abscessed airways that had lost the epithelium were encapsulated and were seen as coagulative necrotic foci that stained intensely for M. bovis, especially at the periphery. Some foci stained weakly and such might have been resolving lesions. Mycoplasma bovis was also demonstrated at sites of mild mononuclear cell infiltration in the livers and kidneys of 2 calves. The mycoplasma was detected within bile ducts in the liver and in the tubular epithelium of the kidney. Abscesses not staining for M. bovis, presumably caused by other pathogens, were seen concurrently with M. bovis-associated abscesses in some lungs. Thirteen other M. bovis-positive cases showed no abscesses, possibly indicating heterogeneity among M. bovis strains. Three other cases with abscesses were negative for M. bovis by culture and immunoperoxidase staining. The monoclonal antibody-based immunohistochemical technique is efficient for specific detection of M. bovis in cases of enzootic pneumonia of calves with or without abscessation. Mycoplasma bovis is implicated in the pathogenesis of lung abscesses in some calves.

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