Radiographic Changes vis-à-vis Tracheo-bronchial Aspirate Cytology Profile in Pneumonic Cattle

Background: The study was conducted to establish the utility of radiography in the diagnosis of lung diseases in cattle and its correlation with different pneumonia diagnosed on the basis of TBA cytology. Methods: Lateral chest radiography and tracheo-bronchial wash was performed in control (n=21) and diseased group (n=55). Diseased group included cattle presented with respiratory signs and diagnosed with pulmonary diseases on the basis of history, physical and clinical examination and tracheo-bronchial wash cytology. Cytologic diagnosis was established as chronic pneumonia (n=24), acute pneumonia (n=18), tuberculosis (n=5) and aspiration pneumonia (n=8). Survivability was also correlated with lung patterns in diseased cattle. Result: Nodular interstitial pattern (27.3%), unstructured interstitial pattern (25.4%), bronchial pattern (20.0%), pleural effusions (12.7%), mixed lung patterns (10.9%) and miliary interstitial pattern (4.54%) was observed in diseased group. Unstructured interstitial pattern and pleural effusions were most evident in acute pneumonia. The radiographic findings in aspiration pneumonia did not correlate well with cytologic findings. Highest survivability was recorded in cows with bronchial pattern (81.8%) and lowest in miliary interstitial pattern (zero per cent).

PROBLEMS OF COMBINING TUBERCULOSIS OF THE RESPIRATORY ORGANS WITH CYSTIC FIBROSIS

Клиническая симптоматика туберкулеза в сочетании с муковисцидозом была обусловлена суммацией проявлений сочетанной патологии и протекала, как тяжелая хроническая пневмония, непрерывно рецидивирующая, с выраженным синдромом обструкции дыхательных путей. Туберкулезное воспаление характеризовалось большей площадью поражения, деструкцией легочной ткани и обнаружением микобактерий туберкулеза в мокроте. Независимо от наличия сопутствующей патологии применялась химиотерапия в стандартном режиме.Цель исследования: оценить течение заболевания и эффективность лечения туберкулезом органов дыхания с учетом наличия сопутствующих заболеваний. Clinical symptoms of tuberculosis in association with cystic fibrosis was determined by summation of the manifestations of comorbidity and proceeded as severe chronic pneumonia, recurrent continuously, with marked airway obstruction syndrome. Tuberculous inflammation was characterized by a larger lesion area, destruction of the lung tissue and the detection of MBT in sputum. Regardless of the presence of concomitant pathology, chemotherapy was used in standard mode.

Strategies of Mycoplasma ovipneumoniae to evade immune clearance by alveolar macrophages

Evidences showed that M. ovipneumoniae might associate with the development and duration of chronic pneumonia. Moreover, sheep infected with M. ovipneumoniae are easily infected by other organisms, suggesting that M. ovipneumoniae may play an immunosuppressive role during infection. However, the mechanism is still poorly understood. The infection occurs in the airway, where resident alveolar macrophages first encounter M. ovipneumoniae. Therefore, primary alveolar macrophages (AMs) were collected from the lungs of healthy adult sheep, and the (iTRAQ) protein assay was used to investigate the immunosuppressive effects of M. ovipneumoniae on sheep AMs. The RAW264.7 cells were used to confirm the findings. The results showed that M. ovipneumoniae promoted higher expression of anti-apoptotic proteins and lower expression of apoptosis-related proteins in the infected AMs. Moreover, the number of infected AMs increased. However, M. ovipneumoniae reduced ATP levels in AMs and impaired late endosome maturation and phagolysosome fusion. Furthermore, M. ovipneumoniae inhibited the autophagy pathway via the Akt-mTOR axis in AMs. These findings indicated that M. ovipneumoniae had distinctive strategies to evade elimination caused by the AMs. The findings might explain the chronic infection and co-infection in sheep infected by M. ovipneumoniae.

Identifikasi Pneumonia Pada Citra Rontgen Paru Menggunakan Metode Power-Law Trans

This paper proposes a system for pneumonia disease classification using X-rays images. This research explores various steps of image processing namely Power-Law Trans, Gabor Wavelet and Boundary. The main aim of this step is to identify infiltrate of human lungs X-Ray images and quantify the infiltration. The result indicates the classification of pneumonia disease into normal, mild pneumonia, and chronic pneumonia.

Rare Case Giant Cystic Mediastinal Tumor (Mature Teratoma) in 57 years old Woman

Background : Incidence of mediastinal teratoma 8,5 - 13,6% of all patients in mediastinal tumor. Teratomas are most common in young adults. Mediastinal teratoma is rarely present clinical manifestation. The clinical manifestation present along with increasing size of mass and also reported with chest pain, back pain, shortness of breath and febrile caused by chronic pneumonia. Rupture of a mediastinal teratoma can cause pleural effusion, empyema and cardiac tamponade. CT is first modality of choice for tumour diagnosis. Mature teratoma is a type of benign tumor, most patients will recovery after complete resection. Case Report : We report one case at Dr. Saiful Anwar hospital, an elderly woman, 57 years old with mediastinal teratoma, whose formerly differential diagnosed with empyema. On the diagnostic test found no evidence of empyema but leads to loculated cloudy pleural effusion. Physical and supporting examination showed anterior mediastinal tumors. Based on the CT-Scan and thorax sonography concluded that is a cystic mass on anterior right hemithorax dextra. The patient performed thoracotomy and eksploration with the final diagnosis of giant cystic mediastinum tumour, with the histopathology is mature teratoma. Conclusion: In this patient, the diagnosis of mediastinal cystic mature teratoma with loculated pleural effusion proven through a multidisciplinary approach covering the pulmonology, thoracic and cardiovascular surgery, radiology and histopathology.

Investigation of Macrolide Resistance Genotypes in Mycoplasma bovis Isolates from Canadian Feedlot Cattle

Mycoplasma bovis is associated with bovine respiratory disease (BRD) and chronic pneumonia and polyarthritis syndrome (CPPS) in feedlot cattle. No efficacious vaccines for M. bovis exist; hence, macrolides are commonly used to control mycoplasmosis. Whole genome sequences of 126 M. bovis isolates, derived from 96 feedlot cattle over 12 production years, were determined. Antimicrobial susceptibility testing (AST) of five macrolides (gamithromycin, tildipirosin, tilmicosin, tulathromycin, tylosin) was conducted using a microbroth dilution method. The AST phenotypes were compared to the genotypes generated for 23S rRNA and the L4 and L22 ribosomal proteins. Mutations in domains II (nucleotide 748; E. coli numbering) and V (nucleotide 2059 and 2060) of the 23S rRNA (rrl) gene alleles were associated with resistance. All isolates with a single mutation at Δ748 were susceptible to tulathromycin, but resistant to tilmicosin and tildipirosin. Isolates with mutations in both domain II and V (Δ748Δ2059 or Δ748Δ2060) were resistant to all five macrolides. However, >99% of isolates were resistant to tildipirosin and tilmicosin, regardless of the number and positions of the mutations. Isolates with a Δ748 mutation in the 23S rRNA gene and mutations in L4 and L22 were resistant to all macrolides except for tulathromycin.

ОZONETHERAPY INFLUENCE ON MORPHOLOGIC CHANGES OF LIVER IN PURULENT INFLAMMATION OF THE LUNGS IN THE EXPERIMENT

Relevance. Ozone increases the oxygen transport function of the blood. The use of ozone therapy for pulmonary pathology is promising. The effect of ozone therapy is associated with the ability of ozone to eliminate hypoxemia and tissue hypoxia, which is always present in patients with pathology of the bronchopulmonary apparatus. Objective: to study morphofunctional changes in liver tissue, in the dynamics of the use of ozonized water in rats in an experimental model of chronic purulent pneumonia.Materials and methods. Three groups were formed from 30 male white rats (180-200 g). In group 1 (n = 10) healthy rats were injected intraperitoneally with 5 ml of ozonated (0.02 mg / L) 0.9% NaCl once a day for 10 minutes. Course 10 days. Animals of the 2 (n = 10) and 3 (n = 10) groups were first modeled for chronic pneumonia. For this, under local novocaine anesthesia, a 1.5-2.0 cm long incision was made on the animal's neck. A nylon thread with a diameter of 0.4 mm and a length of 10-12 cm was inserted into the lumen of the trachea, between its rings, on a thin piercing needle. The distal end of the thread was located in the lumen of the trachea, and its proximal end was fixed on the skin. The wound was sutured tightly in layers. After 45 days, the thread was removed without opening the trachea. Subsequently, animals of group 2 did not receive treatment. And rats of group 3 were injected once a day with 5 ml of ozonized (0.02 mg / l) 0.9% NaCl for 10 minutes. Course 10 days. The animals were removed from the experiment by instant decapitation. The taken pieces of the liver were fixed in formalin. Histological sections were stained with hematoxylin and eosin. Microscopy was performed using an XS-213 light microscope and a Leica microscope. Results. With prolonged irritation of the respiratory tract, structural changes in the liver, characteristic of toxic hepatitis, were revealed. After treatment with ozonated saline, the morphological picture of the liver improved. In healthy rats, ozone therapy did not have a negative effect on the general condition and behavior of the animals. Conclusions. Treatment with ozonated saline improves the morphological picture of the liver of rats with chronic pneumonia.

INTRAREGIONAL PROPAGATION OF COVID-19 CASES IN PARÁ, BRAZIL. ASSESSMENT OF ISOLATION REGIME TO LOCKDOWN

Due to the high incidence of COVID-19 case numbers internationally, the World Health Organization (WHO) declared a Public Health Emergency of global relevance, advising countries to follow protocols to combat pandemic advance through actions that can reduce spread and consequently avoid a collapse in the local health system. On March 18, 2020, Pará notified the first case of COVID-19. After seven weeks, the number of confirmed cases reached 4,756 with 375 deaths. Knowing that infected people may be asymptomatic, the disease symptomatology absence and the population’s neglect of isolation influence the spread, and factors such as chronic pneumonia, high age, obesity, chronic kidney diseases and other comorbidities favor the mortality rate. On the other hand, social isolation, quarantine and lockdown seek to contain the intraregional contagion advance. This study analyzes the dynamics of COVID-19 new cases advance among municipalities in the state of Pará, Brazil. The results show it took 49 days for 81% of the state’s municipalities to register COVID-19 cases. The association between social isolation, quarantine and lockdown as an action to contain the infection was effective in reducing the region’s new cases registration of COVID-19 in the short-term.