Processing Bone Marrow with a Semiautomated Cell Processor for Pediatric Transplants: A Comparison of Two Buffy Coat Preparation Methods

1994 ◽  
Vol 3 (1) ◽  
pp. 59-64 ◽  
Author(s):  
MORRIS KLETZEL ◽  
MARIE L. OLSZEWSKI ◽  
GEORGE F. DUNN
Keyword(s):  
Bone Marrow ◽  
Buffy Coat ◽  
Cell Processor ◽  
Preparation Methods

scholarly journals Evaluation of qPCR on blood and skin microbiopsies, peripheral blood buffy coat smear, and urine antigen ELISA for diagnosis and test of cure for visceral leishmaniasis in HIV-coinfected patients in India: a prospective cohort study

BMJ Open ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. e042519
Author(s):  
Sophie I Owen ◽  
Sakib Burza ◽  
Shiril Kumar ◽  
Neena Verma ◽  
Raman Mahajan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Hiv Infection ◽  
Peripheral Blood ◽  
Medical Science ◽  
Bone Marrow Aspiration ◽  
Buffy Coat ◽  
Tropical Medicine ◽  
Urine Antigen ◽  
Antigen Elisa

IntroductionHIV coinfection presents a challenge for diagnosis of visceral leishmaniasis (VL). Invasive splenic or bone marrow aspiration with microscopic visualisation of Leishmania parasites remains the gold standard for diagnosis of VL in HIV-coinfected patients. Furthermore, a test of cure by splenic or bone marrow aspiration is required as patients with VL-HIV infection are at a high risk of treatment failure. However, there remain financial, implementation and safety costs to these invasive techniques which severely limit their use under field conditions.Methods and analysisWe aim to evaluate blood and skin qPCR, peripheral blood buffy coat smear microscopy and urine antigen ELISA as non-invasive or minimally invasive alternatives for diagnosis and post-treatment test of cure for VL in HIV-coinfected patients in India, using a sample of 91 patients with parasitologically confirmed symptomatic VL-HIV infection.Ethics and disseminationEthical approval for this study has been granted by The Liverpool School of Tropical Medicine, The Institute of Tropical Medicine in Antwerp, the University of Antwerp and the Rajendra Memorial Research Institute of Medical Science in Patna. Any future publications will be published in open access journals.Trial registration numberCTRI/2019/03/017908.

scholarly journals Enhanced recovery of gut-associated lymphoid tissue by infusion of buffy coat cells and gut leukocytes in the murine syngeneic bone marrow transplantation model

Blood ◽  
1986 ◽  
Vol 68 (5) ◽  
pp. 1003-1007
Author(s):  
GS Leventon ◽  
SS Kulkarni ◽  
RA White ◽  
AR Zander
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Marrow Transplantation ◽  
Plasma Cells ◽  
Enhanced Recovery ◽  
Intraepithelial Lymphocytes ◽  
Buffy Coat ◽  
Minimal Dose ◽  
Cell Decline ◽  
Maximal Plasma

Ways of accelerating recovery of the mucous membrane immune system in lethally irradiated mice following syngeneic bone marrow transplantation were studied over a 35-day period by quantification of jejunal intraepithelial lymphocytes (IELs) and lamina propria plasma cells. Recovery after a low bone marrow dose allowing 100% animal survival (LBM) was compared with a high (five times minimal) dose (HBM), or a minimal dose augmented with equal numbers of buffy coat cells (LBM + BC) or small gut mucosal lymphocytes (LBM + GL). The maximal decline and subsequent peak repopulation of IELs were: LBM, days 7 through 14, peaking suboptimally by day 28; HBM, day 14, peaking suboptimally but higher than LBM by day 35; LBM + BC, days 11 through 14, peaking at control levels by day 35; and LBM + GL, day 7, peaking at control levels by day 28. The IEL decline was most severe with LBM and HBM treatment and least with LBM + GL. All transplant groups experienced maximal plasma cell decline by day 7. LBM had the most severe depletion, and LBM + GL had the least. Recovery to control levels for the LBM, HBM, LBM + BC and LBM + GL groups occurred by days 28, 21, 21, and 14, respectively. In all instances, greater than 95% of the plasma cells were IgA positive.

scholarly journals Donor leukocyte transfusions for treatment of recurrent chronic myelogenous leukemia in marrow transplant patients

Blood ◽  
1990 ◽  
Vol 76 (12) ◽  
pp. 2462-2465 ◽  
Author(s):  
HJ Kolb ◽  
J Mittermuller ◽  
C Clemm ◽  
E Holler ◽  
G Ledderose ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Marrow Transplantation ◽  
Chronic Myelogenous Leukemia ◽  
Adoptive Immunotherapy ◽  
Marrow Transplant ◽  
Buffy Coat ◽  
Myelogenous Leukemia ◽  
Graft Versus Host ◽  
Transplant Patients ◽  
Cytogenetic Remission

Abstract Three patients with hematologic relapse after bone marrow transplantation for chronic myelogenous leukemia were treated with interferon alpha and transfusion of viable donor buffy coat. All had complete hematologic and cytogenetic remission, which persisted 32 to 91 weeks after treatment. In two patients graft-versus-host disease developed and was treated by immunosuppression. These results are an example of adoptive immunotherapy without cytoreductive chemotherapy or radiotherapy in human chimeras.

scholarly journals QBC® for the diagnosis of human and canine american visceral leishmaniasis: preliminary data

2001 ◽  
Vol 34 (6) ◽  
pp. 577-581 ◽  
Author(s):  
Daniel B. Liarte ◽  
Ivete L. Mendonça ◽  
Francisco C.O. Luz ◽  
Elza A.S. de Abreu ◽  
Gustavo W.S. Mello ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Visceral Leishmaniasis ◽  
Preliminary Data ◽  
Peripheral Blood ◽  
Buffy Coat ◽  
Promising Method ◽  
Blood Parasites ◽  
Leishmania Chagasi ◽  
Asymptomatic Carriers ◽  
Fluorescent Method

"Quantitative Buffy Coat" (QBC®) is a direct and fast fluorescent method used for the identification of blood parasites. Since Leishmania chagasi circulates in blood, we decided to test it in American visceral leishmaniasis (AVL). Bone marrow (BM) and peripheral blood (PB) of 49 persons and PB of 31 dogs were analyzed. QBC® was positive in BM of 11/11 patients with AVL and in 1/6 patients with other diseases. Amastigotes were identified in PB of 18/22 patients with AVL and in none without AVL. The test was positive in 30 out of the 31 seropositive dogs and in 28/28 dogs with Leishmania identified in other tissues. QBC® is a promising method for diagnosis of human AVL, and possibly for the exam of PB of patients with AVL/AIDS, for the control of the cure and for the identification of asymptomatic carriers. Because it is fast and easy to collect and execute, QBC® should be evaluated for programs of reservoir control.

scholarly journals Production of Macroglobulins in Vitro and a Study of Their Cellular Origin

Blood ◽  
1962 ◽  
Vol 20 (1) ◽  
pp. 56-64 ◽  
Author(s):  
DOROTHEA ZUCKER-FRANKLIN ◽  
EDWARD C. FRANKLIN ◽  
NORMAN S. COOPER
Keyword(s):  
Bone Marrow ◽  
Tissue Culture ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Plasma Cells ◽  
Buffy Coat ◽  
Cellular Origin

Abstract Lymph nodes of three patients with macroglobulinemia of Waldenström were studied in tissue culture and shown to synthesize 19S γ-globulin in vitro. Lymph node imprints, bone marrow, and buffy coat smears of the same patients consisted almost entirely of lymphocytes. When these were stained with fluorescein-conjugated antiserum to macroglobulin, large and medium-sized lymphocytes and lymphoblasts rather than mature lymphocytes or plasma cells were shown to contain the protein. It is suggested that 19S γ-globulin may also be synthesized by cells belonging to the lymphoid series under normal circumstances.

Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction

2007 ◽  
Vol 36 (3) ◽  
pp. 243-247 ◽  
Author(s):  
Joan Cid ◽  
Montse Claparols ◽  
Asunción Pinacho ◽  
José Manuel Hernández ◽  
Pilar Ortiz ◽  
...  
Keyword(s):  
Whole Blood ◽  
Buffy Coat ◽  
Blood Component ◽  
Preparation Methods

scholarly journals Cyanocobalamin, Ascorbic Acid and Pteroylglutamates in Normal and Megaloblastic Bone Marrow

Blood ◽  
1960 ◽  
Vol 15 (3) ◽  
pp. 376-387 ◽  
Author(s):  
E. V. COX ◽  
D. M. MATTHEWS ◽  
M. J. MEYNELL ◽  
W. T. COOKE ◽  
R. GADDIE
Keyword(s):  
Bone Marrow ◽  
Ascorbic Acid ◽  
Folic Acid ◽  
Pernicious Anemia ◽  
Megaloblastic Anemia ◽  
Normal Subjects ◽  
Causative Factor ◽  
Buffy Coat ◽  
Plasma Ascorbic Acid ◽  
Therapeutic Implications

Abstract The B12 activity as estimated by Lactobacillus leichmannii, the folic-acid-like activity by Streptococcus faecalis (F.A.A.) and the ascorbic acid concentration have been determined in the blood and buffy coat of bone marrow of normal subjects, 10 patients with pernicious anemia in relapse, a group of patients with non-Addisonian megaloblastic anemia and some patients with iron deficiency. A correlation between the serum B12 and the plasma ascorbic acid and their respective levels in bone marrow was observed. The marrow and serum B12 levels in prenicious anemia were abnormally low, but they did not differ from a group of 5 patients with hypochromic normoblastic anemia who had both low serum and marrow levels. The concentration of F.A.A. in the marrow of patients with pernicious anemia was reduced, but it was felt that this was more likely a manifestation of the megaloblastic anemia rather than a causative factor. One of six patients with megaloblastic anemia of pregnancy had no detectable deficiency, while the other five had reduced B12, folic acid and ascorbic acid concentrations. The possible therapeutic implications are discussed. There was a significant reduction in the bone marrow concentration of ascorbic acid in all patients with megaloblastic anemia.

scholarly journals Circulating and bone marrow myeloid cells containingLeishmaniaamastigotes in a case of advanced canine leishmaniosis

2019 ◽  
Vol 31 (5) ◽  
pp. 726-731
Author(s):  
Ioannis L. Oikonomidis ◽  
Theodora K. Tsouloufi ◽  
Mathios E. Mylonakis ◽  
Dimitra Psalla ◽  
Nectarios Soubasis ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Weight Loss ◽  
Blood Smear ◽  
Bone Marrow Aspiration ◽  
Buffy Coat ◽  
Routine Practice ◽  
Canine Leishmaniosis ◽  
Aspiration Cytology ◽  
Blood Smears ◽  
Leishmania Amastigotes

A 5-y-old male Poodle mix was presented with intermittent vomiting, anorexia, and weight loss. Physical examination revealed emaciation, lethargy, dehydration, hypothermia, respiratory distress, and splenomegaly. Based on clinicopathologic, serologic, and parasitologic findings, diagnoses of severe leishmaniosis and dirofilariasis were made. Extracellular, intraneutrophilic, and intramonocytic Leishmania amastigotes were observed on blood smear and buffy coat smear examination. In blood smears, 0.2% of neutrophils were observed to be infected; in buffy coat smears, 0.5% of neutrophils and 0.1% of monocytes were found to be infected. Leishmania amastigotes were also found engulfed by eosinophils and neutrophil precursors in bone marrow aspiration cytology. The detection of Leishmania amastigotes in blood smears is rare, and the clinical significance is uncertain. In circulating blood, Leishmania amastigotes are primarily found phagocytized by neutrophils. Although debatable, there is growing evidence that neutrophils are used as carriers enabling the “silent entry” of the protozoa into macrophages (“Trojan horse” theory). To date, cytologic screening of blood smears for the diagnosis of canine leishmaniosis is not a routine practice. Clinical pathologists and practitioners should be aware that Leishmania amastigotes may be present in neutrophils and less frequently monocytes during blood smear evaluation; neutrophil precursors and eosinophils may also be parasitized in bone marrow specimens.

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