Multiple Injections of Autologous Adipose-Derived Stem Cells Accelerate the Burn Wound Healing Process and Promote Blood Vessel Regeneration in a Rat Model

Bee pollen is a valuable apitherapeutic product greatly appreciated by the natural medicine because of its potential medical and nutritional applications. It demonstrates a series of actions such as antifungal, antimicrobial, antiviral, anti-inflammatory, hepatoprotective, anticancer immunostimulating, and local analgesic. Its radical scavenging potential has also been reported. Beneficial properties of bee pollen and the validity for their therapeutic use in various pathological condition have been discussed in this study and with the currently known mechanisms, by which bee pollen modulates burn wound healing process.

Download Full-text

Autologous and not allogeneic adipose-derived stem cells improve acute burn wound healing

PLoS ONE ◽

10.1371/journal.pone.0197744 ◽

2018 ◽

Vol 13 (5) ◽

pp. e0197744 ◽

Cited By ~ 14

Author(s):

Yu-Wei Chang ◽

Yi-Chia Wu ◽

Shu-Hung Huang ◽

Hui-Min David Wang ◽

Yur-Ren Kuo ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Adipose Derived Stem Cells ◽

Burn Wound ◽

Burn Wound Healing

Download Full-text

Propolis Modulates Fibronectin Expression in the Matrix of Thermal Injury

BioMed Research International ◽

10.1155/2014/748101 ◽

2014 ◽

Vol 2014 ◽

pp. 1-10 ◽

Cited By ~ 14

Author(s):

Pawel Olczyk ◽

Katarzyna Komosinska-Vassev ◽

Grzegorz Wisowski ◽

Lukasz Mencner ◽

Jerzy Stojko ◽

...

Keyword(s):

Wound Healing ◽

Healing Process ◽

Silver Sulfadiazine ◽

Wound Healing Process ◽

Burn Wound ◽

Burn Treatment ◽

Burn Wound Healing ◽

Fibronectin Expression ◽

The Matrix ◽

Healing Wounds

The aim of the study was to assess the propolis effect on fibronectin metabolism in the course of burn wounds healing process. A model of burn wound healing of pig skin was applied. The amount of the released glycoprotein was assessed by a surface plasmon resonance. The profile of extracted fibronectin components was also assessed by an electrophoresis in polyacrylamide gel, with a subsequent immunodetection by Western Blotting. Propolis burn treatment decreased the release of fibronectin components from healing wounds in relation to damages treated with silver sulfadiazine. The main reason of decreased extraction of fibronectin components from wounds treated with propolis was a substantial decrease of degradation product release of the mentioned glycoprotein, which was observed particularly from the 3rd to 5th day of the repair. Wounds treatment with propolis demonstrated, especially in relation to damages treated with silver sulfadiazine, the decreased release of synthesized fibronectin molecules. The obtained results suggest that propolis modifies fibronectin metabolism in the course of wound healing process. The influence of propolis is reflected in prevention of fibronectin biosynthesis as well as its degradation in the wound area. The above-mentioned metabolic changes may decrease the risk of complications in the repair wounds process.

Download Full-text

Adipose-derived stem cells improve grafted burn wound healing by promoting wound bed blood flow

Burns & Trauma ◽

10.1093/burnst/tkaa009 ◽

2020 ◽

Vol 8 ◽

Cited By ~ 1

Author(s):

Osamu Fujiwara ◽

Anesh Prasai ◽

Dannelys Perez-Bello ◽

Amina El Ayadi ◽

Irene Y Petrov ◽

...

Keyword(s):

Stem Cells ◽

Wound Healing ◽

Blood Flow ◽

Topical Application ◽

Enzyme Linked Immunosorbent Assay ◽

Adipose Derived Stem Cells ◽

Burn Wound ◽

Burn Wound Healing ◽

Cell Based Therapy ◽

Donor Skin

Abstract Background Researchers have explored the use of adipose-derived stem cells (ASCs) as a cell-based therapy to cover wounds in burn patients; however, underlying mechanistic aspects are not completely understood. We hypothesized that ASCs would improve post-burn wound healing after eschar excision and grafting by increasing wound blood flow via induction of angiogenesis-related pathways. Methods To test the hypothesis, we used an ovine burn model. A 5 cm2 full thickness burn wound was induced on each side of the dorsum. After 24 hours, the burned skin was excised and a 2 cm2 patch of autologous donor skin was grafted. The wound sites were randomly allocated to either topical application of 7 million allogeneic ASCs or placebo treatment (phosphate-buffered saline [PBS]). Effects of ASCs culture media was also compared to those of PBS. Wound healing was assessed at one and two weeks following the application of ASCs. Allogeneic ASCs were isolated, cultured and characterized from non-injured healthy sheep. The identity of the ASCs was confirmed by flow cytometry analysis, differentiation into multiple lineages and gene expression via real-time polymerase chain reaction. Wound blood flow, epithelialization, graft size and take and the expression of vascular endothelial growth factor (VEGF) were determined via enzyme-linked immunosorbent assay and Western blot. Results Treatment with ASCs accelerated the patch graft growth compared to the control (p < 0.05). Topical application of ASCs significantly increased wound blood flow (p < 0.05). Expression of VEGF was significantly higher in the wounds treated with ASCs compared to control (p < 0.05). Conclusions ASCs accelerated grafted skin growth possibly by increasing the blood flow via angiogenesis induced by a VEGF-dependent pathway.

Download Full-text

An Overview of the Most Important Medicinal Plants Native to Iran by Property of Burn Wound Healing: A Systematic Review

International Journal of Current Pharmaceutical Review and Research ◽

10.25258/ijcprr.v8i02.9192 ◽

2017 ◽

Vol 8 (02) ◽

Author(s):

Mehrdad Karimi ◽

Mahnaz Mardani ◽

Leila Mahmoodnia

Keyword(s):

Wound Healing ◽

Medicinal Plants ◽

Cinnamic Acid ◽

Healing Process ◽

Review Article ◽

The Body ◽

Wound Healing Process ◽

Burn Wound ◽

Burn Wounds ◽

Burn Wound Healing

Burn is an injury where the skin is destroyedby various factors such as heat, cold, electricity, etc.When skin injuryoccurs, pathogens can invade the body and burn wounds get infected shortly after the damage. The wound healing process is a process done by the coordination of tissues, cells and various factors. In the acute phase, inflammatory mechanism of burns has negative effects due to capillary leak. On the other hand, in the later stages of burn,inflammation is necessary for wound healing. Silver nitrate, silver sulfadiazine, and Mafenide acetate are also used for wound care. These compounds cause delayed wound healing and have cytotoxic effects. Iran is among the countries that have rich traditional medicine and herbal treatment of burn wounds in history. Therefore, in this review article, we tried to report the medicinal plants native to Iran which are effective for burn wounds. In this review article, by searching databases such as Scopus, Google Scholar, Spy DVD, mag Iran and… the articles were searched by searching keywords including burn wounds, herbs, extracts, essences and Iran. After finally searching, it was determined that medicinal plants below are the most important medicinal plants native to Iran that affect burn wounds. 11 Medicinal herbs 11 Abukhalsa plant, chamomile, oak, hungry for love, green tea, purslane, cinnamon, Hypericum, and Commiphora are the most important Medicinal Plants which are effective for burn wound healing in Iran. Alkanin, Naphthoquinone and Shikonin, tannins, pectin, alkaloids, saponins and tannins fixed, resin and vitamin C, flavonoids rutin, aucubin and catalpolCatechin, Epicatechin, EpicatechinGallate, Epigallocatechin and Epigallocatechin-gallate, oxalic acid, Cinnamic acid, caffeic acid , maleic acid, Citric acid, coumarin, flavonoids, alanine, tannins, alpha-linolenic acid, cinnamic acid, phenolic compounds such as eugenol, and safrolephellandrene, terpene compounds such as limonene and linalool, trans-cinnamaldehyde (MDA), tannins, coumarin, resin, Cinema-hydroxy phenyl propane compounds such as formaldehyde and mannitol, curcumin, terpenoids, steroids, Flavonoids, lignans are active compounds of plant bioactive substances that are effective on burn wound healing.

Download Full-text

Role of topical Sucralfate in healing of burn wounds

International Surgery Journal ◽

10.18203/2349-2902.isj20183409 ◽

2018 ◽

Vol 5 (9) ◽

pp. 2995

Author(s):

Mahim Koshariya ◽

Abhishek Shitole ◽

Vibhore Agarwal ◽

S. Dave

Keyword(s):

Wound Healing ◽

Secondary Infection ◽

Healing Process ◽

Gastrointestinal Diseases ◽

Wound Healing Process ◽

Burn Patients ◽

Burn Wound ◽

Burn Wound Healing ◽

Group 2 ◽

Group 1

Background: Sucralfate is a basic aluminum salt of sucrose octasulphate which was orally taken for prevention and treatment of several gastrointestinal diseases. This study primarily aims to analyze whether sucralfate accelerates wound healing process in burn patients. The incidence of infection & relieve in pain in burn patients was also compared.Methods: This is an observational study carried out in the Department of General Surgery, Hamidia Hospital Bhopal on 50 patients divided into group 1 (sucralfate)and group 2 [a-sucralfate; b-silver sulfadiazine (SSD)]. Demographics, history, physical, and systemic examinations of the patients were recorded.Results: It was observed that sucralfate augments the formation of granulation tissue (in 6-17 days) as compared to SSD (14-22 days). It was noticed that faster healing by re-epithelialization was present in sucralfate group (11-22 days) as compared with SSD group (15-30 days). By the end of 3rd week 50-75% of wound was healed in sucralfate group as compared with 35-50% in SSD group. Incidence of secondary infection was less when topical sucralfate was used (group 1 = 25%; group 2a = 16.6%; group 2b = 66.66%). There was a marked relief in pain and discomfort after sucralfate application as compared to SSD.Conclusions: Using topical sucralfate expedite the burn wound healing process, significantly decreases pain with no local or systemic adverse reactions to the topical application therefore it can be used as an adjunctive or alternative agent in the future. However, multicentric trials with larger sample size are needed to insure the concept.

Download Full-text